Abstract：
:Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals reflecting their diverse applications in research and the clinic. The correct glycosylation of mAbs is required to elicit effector functions such as complement-dependent and antibody-dependent cell-mediated cytotoxicity, although these may be undesirable for the treatment of certain chronic diseases. To gain insight into the properties of glycan-deficient mAbs, we generated and characterized six different aglycosylated human IgG1 mAbs (carrying the N297A mutation) and compared them to their glycosylated counterparts. We found no differences in solubility or heterogeneity, and all mAbs the remained stable in stress tests at 4 and 37 °C. Surface plasmon resonance spectroscopy showed no differences in binding affinity, and the in vivo terminal serum half-life and plasma clearance were similar in rats. However, differential scanning calorimetry revealed that the aglycosylated mAbs contained a less stable C(H)2 domain and they were also significantly more susceptible to pH-induced aggregation. We conclude that aglycosylated mAbs are functionally equivalent to their glycosylated counterparts and could be particularly suitable for certain therapeutic applications, such as the treatment of chronic diseases.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Hristodorov D,Fischer R,Joerissen H,Müller-Tiemann B,Apeler H,Linden Ldoi
10.1007/s12033-012-9531-xsubject
Has Abstractpub_date
2013-03-01 00:00:00pages
326-35issue
3eissn
1073-6085issn
1559-0305journal_volume
53pub_type
杂志文章abstract：:The major online single nucleotide polymorphism (SNP) databases freely available as research tools for genetic analysis are explained, reviewed, and compared. An outline is given of the search strategies that can be used with the most extensive current SNP databases: National Centre for Biotechnology Information (NCBI...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-009-9150-3
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journal_title：Molecular biotechnology
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doi：10.1385/MB:31:3:203
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doi：10.1007/s12033-012-9592-x
更新日期：2013-06-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:21:2:105
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-011-9486-3
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9770-0
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abstract：:Human recombinant vascular endothelial growth factor-A121 (hrVEGF-A121) has applications in pharmaceutical industry especially in regenerative medicine. Here, we report the expression, purification, and characterization of hrVEGF-A121 in Escherichia coli expression system using human small ubiquitin-related modifier-3...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-018-0094-3
更新日期：2018-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s12033-016-9923-4
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更新日期：2009-10-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
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更新日期：2001-01-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02907471
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9739-z
更新日期：2014-07-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-015-9875-0
更新日期：2015-09-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-020-00290-8
更新日期：2021-02-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-010-9365-3
更新日期：2011-07-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-018-0104-5
更新日期：2018-09-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/mb:26:1:17
更新日期：2004-01-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
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journal_title：Molecular biotechnology
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journal_title：Molecular biotechnology
pub_type： 杂志文章
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02789331
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
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journal_title：Molecular biotechnology
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02921609
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-013-9714-0
更新日期：2014-04-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-018-0140-1
更新日期：2019-02-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-013-9659-3
更新日期：2013-10-01 00:00:00
abstract：:In this paper, we report cloning of a pectate lyase gene from Bacillus amyloliquefaciens S6 (pelS6), and biochemical characterization of the recombinant pectate lyase. PelS6 was found to be identical with B. subtilis 168 pel enzyme with 100% amino acid sequence homology. Although these two are genetically very close, ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-019-00194-2
更新日期：2019-09-01 00:00:00
