Abstract:
:Jet-injection has become an applicable technology among other established nonviral delivery systems, such as particle bombardment or in vivo electroporation. The low-volume jet injector employed in this study uses compressed air to inject solutions of 1.5-10 microL containing naked DNA into the desired tissue. The novel design of this prototype makes multiple jet-injections possible. Therefore, repeated jet-injections into one target tissue can be performed easily. This jet-injector hand-held system was used for the direct in vivo gene transfer of plasmid DNA into tumors to achieve efficient expression of reporter genes (beta-galactosidase, green fluorescent protein [GFP]) and of therapeutic genes (TNF-alpha) in different tumor models. The study presented here revealed the key parameters of efficient in vivo jet-injection (jet-injection volume, pressure, jet penetration, DNA stability) to define the optimal conditions for a jet-injection-aided nonviral gene therapy.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Walther W,Stein U,Fichtner I,Voss C,Schmidt T,Schleef M,Nellessen T,Schlag PMdoi
10.1385/MB:21:2:105keywords:
subject
Has Abstractpub_date
2002-06-01 00:00:00pages
105-15issue
2eissn
1073-6085issn
1559-0305pii
MB:21:2:105journal_volume
21pub_type
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