Abstract：
:To develop combined vaccine systems, we have generated Edwardsiella tarda ghosts (ETG) displaying a foreign protein on the outer membrane and also Ed. tarda cadaver (ETC) expressing a heterologous protein in the cytoplasm. Green fluorescent protein (GFP) was used as a model foreign protein. A constitutive promoter (EtPR C28-1) cloned newly from Ed. tarda was used as a promoter for the expression of foreign protein. Comparison of the strength of the new promoter with a commercially available constitutive promoter (P(HCE)) showed higher expression levels of the novel expression system. The N-terminal domain of ice nucleation protein (InaN), an outer membrane protein of Pseudomonas syringae, was used as an anchor motif for surface display of GFP. By transformation of Ed. tarda with the constructed vectors, GFP was successfully expressed on the surface of ETG and in the cytoplasm of ETC. When compared to P(HCE) driven expression, approximately more than 2 times of GFP was expressed on ETG and in ETC by EtPR C28-1 promoter when judged by fluorescent spectrophotometry. Furthermore, significantly higher expression of GFP on the surface of ETG by EtPR C28-1 than by P(HCE) was demonstrated by serum agglutination assay. These results suggest that the newly cloned Ed. tarda constitutive promoter is capable to express foreign proteins not only on the surface of Ed. tarda ghosts but also in the cytoplasm of Ed. tarda cadavers, and can be used as an efficient promoter for the expression of heterologous antigens of the ETG and ETC-based combined vaccines.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Choi SH,Nam YK,Kim KHdoi
10.1007/s12033-010-9277-2subject
Has Abstractpub_date
2010-10-01 00:00:00pages
127-33issue
2eissn
1073-6085issn
1559-0305journal_volume
46pub_type
杂志文章abstract：:PREP1/p160 is a protein complex with relevant physiopathological roles in vivo. p160 regulates PREP1 transcriptional activity by preventing the formation of other PREP1-containing complexes, whereas PREP1 regulates p160 activity by increasing its stability. This induces the repression of the insulin-regulated glucose ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-016-9932-3
更新日期：2016-05-01 00:00:00
abstract：:Heat shock proteins (HSPs) are upregulated in response to stress and play a protective function in refolding of cellular proteins. In hypertension, the heart is a vital organ that requires examination and investigation, and primary induction of HSPs is predominantly effected. Hypertension results from osmotic imbalanc...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-009-9144-1
更新日期：2009-06-01 00:00:00
abstract：:An alternative method to combine mutagenesis PCR with dITP and fragmentation by endonuclease V for directed evolution was developed. In comparison to the routine protocol for directed evolution, dITP was used as mutation reagent in the mutagenesis PCR. Subsequently, the incorporated dITP in the PCR products could repr...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-012-9516-9
更新日期：2013-01-01 00:00:00
abstract：:Pasteurella multocida is the main cause of haemorrhagic septicaemia (HS) outbreak in livestock, such as cattle and buffaloes. Conventional vaccines such as alum-precipitated or oil-adjuvant broth bacterins were injected subcutaneously to provide protection against HS. However, the immunity developed is only for short ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-020-00244-0
更新日期：2020-05-01 00:00:00
abstract：:Several mutations were introduced into the Cry1Ac toxin gene, resulting in four variants with altered sequences that were responsible for low expression of the toxin in transgenic plants. These variants were as follows: V1, with modified three A/T-rich regions, including the first signal of transcription termination; ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/mb:26:1:17
更新日期：2004-01-01 00:00:00
abstract：:Laccases are polyphenol oxidoreductases used in a number of industrial applications. Due to the increasing demand for these "green catalysis" enzymes, the identification and biochemical characterisation of their novel properties is essential. In our study, cloned Madurella mycetomatis laccase (mmlac) genes were hetero...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-020-00281-9
更新日期：2021-01-01 00:00:00
abstract：:Cardiomyocytes are terminally differentiated cells that to date have been characterized as poor targets for nonviral gene transfer. This study was therefore designed to determine the optimal nonviral gene transfer parameters in cell cultures of neonatal rat cardiomyocytes and to compare them with the efficiency of gen...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:28:1:21
更新日期：2004-09-01 00:00:00
abstract：:While conducting our research on mutations in the human blood platelet glycoprotein Ib-alpha (GPIbalpha) gene, we detected an unusual deletion of 84 bp. This deletion took place in vitro, during PCR and between two direct repeats. It was observed that the deletion could be detected either by the direct sequencing of t...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-008-9059-2
更新日期：2008-09-01 00:00:00
abstract：:Hypermethylated in Cancer-1 (HIC-1) is a novel tumor suppressor plays crucial role in tumor formation through loss of function by hypermethylation. HIC-1 is known as transcriptional factor whereas little known about its structure and function. Requirement felt to clone and express full coding protein and reveal variou...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9783-8
更新日期：2014-11-01 00:00:00
abstract：:Baculovirus expression vector system (BEVS) is widely known as a mass-production tool to produce functional recombinant glycoproteins except that it may not be always suitable for medical practice due to the differences in the structure of N-linked glycans between insects and mammalian. Currently, various approaches h...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-017-0003-1
更新日期：2017-05-01 00:00:00
abstract：:The electron microscopic study of DNA-protein complexes can yield valuable information that is often not easily available by other methods. In this article we give a number of examples that were chosen to illustrate the utility of electron microscopy. Along with the strategy used are protocols that allow such experime...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:16:1:77
更新日期：2000-09-01 00:00:00
abstract：:We investigated the efficiency of a single plasmid transfection along the longitudinal axis of the regenerating soleus of young rats. This also reflected transfection efficiency along the fibers because the soleus is a nearly fusiform muscle in young animals. The complete regeneration was induced by notexin and the tr...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-012-9555-2
更新日期：2013-06-01 00:00:00
abstract：:The MYB proteins constitute one of the largest transcription factor families in plants. Much research has been performed to determine their structures, functions, and evolution, especially in the model plants, Arabidopsis, and rice. However, this transcription factor family has been much less studied in wheat (Triticu...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-011-9486-3
更新日期：2012-10-01 00:00:00
abstract：:A computer program (PCBI) was developed to quickly calculate codon bias index (CBI). PCBI can analyze a gene containing introns. The 22 preferred codons defined from Saccharomyces cerevisiae were used in PCBI as the standard to measure the CBI values. However, users can modify the preferred codons to suit each organis...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02760858
更新日期：1998-10-01 00:00:00
abstract：:Enzymes isolated from extremophiles often exhibit superior performance and potential industrial applications. There are several advantages performing biocatalysis at elevated temperatures, including enhanced reaction rates, increased substrate solubility and decreased risks of contamination. Furthermore, thermophilic ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-016-9975-5
更新日期：2016-11-01 00:00:00
abstract：:The monomeric bacteriophage RNA polymerases allow the synthesis of virtually any RNA molecule in unlimited quantity. In this protocol, we describe the preparation of plasmid and PCR-derived templates. A basic transcription protocol is provided with several optional modifications. The use of RNA probes in Northern blot...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02761751
更新日期：1997-04-01 00:00:00
abstract：:The Delta12 desaturase represents a diverse gene family in plants and is responsible for conversion of oleic acid (18:1) to linoleic acid (18:2). Several members of this family are known from plants like Arabidopsis and Soybean. Using primers from conserved C- and N-terminal regions, we have cloned a novel Delta12 des...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-009-9150-3
更新日期：2009-06-01 00:00:00
abstract：:Many phytopathogenic micro-organisms such as bacteria and fungi produce pectin methylesterases (PME) during plant invasion. Plants and insects also produce PME to degrade plant cell wall. In the present study, a thermostable pectin methylesterase (CtPME) from Clostridium thermocellum belonging to family 8 carbohydrate...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-017-9997-7
更新日期：2017-05-01 00:00:00
abstract：:In this study, host-specific forms of the blast pathogen Magnaporthe oryzae in sub-Saharan Africa (SSA) were characterised from distinct cropping locations using a combination of molecular and biological assays. Finger millet blast populations in East Africa revealed a continuous genetic variation pattern and lack of ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-011-9429-z
更新日期：2012-02-01 00:00:00
abstract：:The sorbents with immobilized histidine as a pseudo affinity ligand with a wide specificity is described. The possibilities and relevant chemistries to use both particulate and flat or hollow fiber membranes as support matrices are discussed. The usefulness of such adsorbents for the purification of a wide variety of ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02761713
更新日期：1996-12-01 00:00:00
abstract：:Biobanks, more formally known as biological resource centers (BRCs), form an "unsung" yet critical component of the infrastructures for scientific research, industry and conservation, without which much of the current scientific activity involving microbial cultures and cell-lines would be effectively impossible. BRCs...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s12033-008-9099-7
更新日期：2008-10-01 00:00:00
abstract：:In this article, four types of standards were assessed in a SYBR Green-based real-time PCR procedure for the quantification of Staphylococcus aureus (S. aureus) in DNA samples. The standards were purified S. aureus genomic DNA (type A), circular plasmid DNA containing a thermonuclease (nuc) gene fragment (type B), DNA...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-011-9417-3
更新日期：2012-01-01 00:00:00
abstract：:D-Stereospecific amidohydrolase (DAH) from Streptomyces sp. 82F2 has potential utility for the synthesis of D/L configuration dipeptides by an aminolysis reaction. Structural comparison of DAH with substrate-bound D-amino acid amidase revealed that three residues located in the active site pocket of DAH (Thr145, Ala26...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-018-0104-5
更新日期：2018-09-01 00:00:00
abstract：:Artificial microRNA (amiRNA) has become a powerful tool for gene silencing in plants. A new method for easy and rapid construction of rice artificial miRNA vector is described. The procedure involved modification of the pCAMBIA1300-UR vector by insertion of a 'vector modification fragment'. This was prepared from the ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-010-9291-4
更新日期：2010-11-01 00:00:00
abstract：:Enamel is the covering tissue of teeth, made of regularly arranged hydroxyapatite crystals deposited on an organic matrix composed of 90% amelogenin that is completely degraded at the end of the enamel formation process. Amelogenin has a biomineralizing activity, forming nanoparticles or nanoribbons that guide hydroxy...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-017-0030-y
更新日期：2017-10-01 00:00:00
abstract：:A detailed method is presented for the creation of head-to-tail multimers of short blunt restriction fragments, ligated into a plasmid vector in a single-tube reaction. Random priming of the concatemer insert readily yields hybridization probes of high specificity, unattainable from the short monomer fragments. ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02789291
更新日期：1994-08-01 00:00:00
abstract：:Terminal Restriction Fragment Length Polymorphism (T-RFLP) or Fluorescent Polymerase Chain Reaction/Restriction Fragment Length Polymorphism (FluRFLP) have made a significant impact on the way in which PCR products amplified from mixed community DNA extracts have been assessed. Technically, these approaches are essent...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:16:3:261
更新日期：2000-11-01 00:00:00
abstract：:UV-B radiation can damage biomolecules, such as DNA, RNA, and proteins, halting essential cellular processes; this damage is partly due to ROS generation. Plant secondary metabolites may protect against UV-B. Psychotria brachyceras Müll. Arg. (Rubiaceae), a subtropical shrub, produces brachycerine, a monoterpene indol...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-012-9546-3
更新日期：2013-05-01 00:00:00
abstract：:This article describes the construction, expression, and purification of RNase single-chain antibody fusion proteins. To construct a fusion protein, the gene for each moiety, the RNase and the binding ligand, is modified separately to contain complementary DNA encoding a 13 amino acid spacer that separates the RNase f...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:20:1:063
更新日期：2002-01-01 00:00:00
abstract：:We report on a unified rapid betaine-based-PCR protocol for amplification of the (CAG)n region in Huntington disease (HD) and the (CGG)n region in Fragile X syndrome (FXS), followed by an electrophoretic separation on automated sequencer for precise determination of the triplet numbers. The high betaine concentration ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-010-9260-y
更新日期：2010-06-01 00:00:00
