Abstract：
:Fibrillation of α-synuclein proceeds through distinct stages, with oligomers combining to form the seed or the nucleus, followed by exponential and saturation phases. Osmolytes are considered to act as protein stabilizers by virtue of their ability to inhibit protein aggregation. Trehalose, a non-reducing disaccharide which is conventionally used as a stabilizer, was found to order α-synuclein, a natively disordered protein, into a non-native conformation such that the protein folding pathway is driven towards aggregation. Thus, by ordering the pathway intermediates, the osmolyte trehalose exerts variable effect on an intrinsically disordered protein when compared with its effect on natively folded proteins.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Naik V,Kardani J,Roy Idoi
10.1007/s12033-016-9923-4subject
Has Abstractpub_date
2016-04-01 00:00:00pages
251-5issue
4eissn
1073-6085issn
1559-0305pii
10.1007/s12033-016-9923-4journal_volume
58pub_type
杂志文章abstract：:Any biopharmaceutical product that has involved the use of animal-derived material during the manufacturing process has the potential to be contaminated with animal viruses. To ensure safety of these products, extensive testing is performed on the starting materials, such as the cell banks, and on the raw materials us...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:21:1:057
更新日期：2002-05-01 00:00:00
abstract：:Laccases are polyphenol oxidoreductases used in a number of industrial applications. Due to the increasing demand for these "green catalysis" enzymes, the identification and biochemical characterisation of their novel properties is essential. In our study, cloned Madurella mycetomatis laccase (mmlac) genes were hetero...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-020-00281-9
更新日期：2021-01-01 00:00:00
abstract：:Translation initiation of Coxsackievirus B3 (CVB3) RNA is directed by an internal ribosome entry site (IRES) within the 5' untranslated region. Host cell factors involved in this process include some canonical translation factors and additional RNA-binding proteins. We have, previously, described that the Sabin3-like ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-012-9592-x
更新日期：2013-06-01 00:00:00
abstract：:The significance of actin cytoskeleton on cell growth was historically studied using toxic drugs, such as cytochalasin. However, it is possible that unpredictable effects of these agents may have influenced the reported observations. In our study, we have established a drug-free system using cofilin overexpression to ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:31:1:001
更新日期：2005-09-01 00:00:00
abstract：:Enamel is the covering tissue of teeth, made of regularly arranged hydroxyapatite crystals deposited on an organic matrix composed of 90% amelogenin that is completely degraded at the end of the enamel formation process. Amelogenin has a biomineralizing activity, forming nanoparticles or nanoribbons that guide hydroxy...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-017-0030-y
更新日期：2017-10-01 00:00:00
abstract：:There is a growing pharmaceutical market for protein-based drugs for use in therapy and diagnosis. The rapid developments in molecular and cell biology have resulted in production of expression systems for manufacturing of recombinant proteins and monoclonal antibodies. These proteins are glycosylated when expressed i...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02745880
更新日期：1994-12-01 00:00:00
abstract：:Recombinant adenovirus vectors have proven to be useful tools in facilitating gene transfer. Construction of such vectors requires a knowledge of the adenovirus genome structure and its life cycle. A commonly used recombinant adenovirus involves deletion of the E1 region; such a recombinant is traditionally produced b...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:18:1:63
更新日期：2001-05-01 00:00:00
abstract：:A detailed method is presented for the creation of head-to-tail multimers of short blunt restriction fragments, ligated into a plasmid vector in a single-tube reaction. Random priming of the concatemer insert readily yields hybridization probes of high specificity, unattainable from the short monomer fragments. ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02789291
更新日期：1994-08-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02752254
更新日期：1997-10-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02907471
更新日期：1995-08-01 00:00:00
abstract：:Yeast artificial chromosome (YAC) cloning systems enable the cloning of DNA stretches of 50 to well over 2000 kb. This makes it possible to study large intact regions of DNA in detail, by restriction mapping the YAC to produce a physical map and by examining the YAC for coding sequences or genes. YACs are important fo...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02921558
更新日期：1994-04-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-013-9689-x
更新日期：2014-02-01 00:00:00
abstract：:Dendritic cell-based vaccines are being evaluated in clinical trials to determine their ability to activate clinically relevant tumor antigen-specific immune responses. Although some groups isolate dendritic cells from peripheral blood, most have found it more efficient to generate large numbers from peripheral blood ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:25:1:95
更新日期：2003-09-01 00:00:00
abstract：:Epoxide hydrolases (EHs; 3.3.2.x) catalyze the enantioselective ring opening of racemic epoxides to the corresponding enantiopure vicinal diols and remaining equivalent unreacted epoxides. These epoxides and diols are used for the synthesis of chiral drug intermediates. With an upsurge in the methods for identificatio...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s12033-017-9996-8
更新日期：2017-03-01 00:00:00
abstract：:Bacteriophage lambda has been in use as a cloning vector for over 25 years, and has been used extensively as an expression vector. The efficiency of packaging and infection, and the simplicity of plaque screening are advantages of lambda as a cloning vector. A number of ingenious modifications help overcome the disadv...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:17:3:219
更新日期：2001-03-01 00:00:00
abstract：:Biobanks, more formally known as biological resource centers (BRCs), form an "unsung" yet critical component of the infrastructures for scientific research, industry and conservation, without which much of the current scientific activity involving microbial cultures and cell-lines would be effectively impossible. BRCs...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s12033-008-9099-7
更新日期：2008-10-01 00:00:00
abstract：:Internal ribosome entry site (IRES) elements are highly structured RNA sequences that function to recruit ribosomes for the initiation of translation. In contrast to the canonical cap-binding, the mechanism of IRES-mediated translation initiation is still poorly understood. Translation initiation of the coxsackievirus...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s12033-013-9674-4
更新日期：2013-10-01 00:00:00
abstract：:RNA interference (RNAi) efficiently induces sequence-specific gene silencing in mammalian cells through short interfering RNA (siRNA) of 21-23 nucleotides synthesized in vitro or expressed by DNA-based vector. However, introduction of siRNA into mammalian cells by transfection limits the application of RNAi, especiall...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:28:3:195
更新日期：2004-11-01 00:00:00
abstract：:Monoclonal antibodies (Mabs) against human chorionic gonadotropin hormone (hCG) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. Sixty-five percent of the total culture wells exhibited hybrid growth and 8% of the total wells (13 culture wells) contained anti-hCG secreting hybrids. A pos...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:17:2:119
更新日期：2001-02-01 00:00:00
abstract：:In this review I briefly describe the technique of protein engineering and indicate how the present state of knowledge allows proteins to be mutated to increase or decrease stability. I discuss experiments on both model proteins and those of relevance to the food industry and show how hydrophobic forces are a major dr...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02921609
更新日期：1995-10-01 00:00:00
abstract：:Recent human clinical trials results demonstrated successful treatment for certain genetic forms of cystic fibrosis (CF). To extend treatment opportunities to those afflicted with other genetic forms of CF disease, structural and biophysical characterization of CF transmembrane conductance regulator (CFTR) is urgently...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9830-5
更新日期：2015-05-01 00:00:00
abstract：:Human adipose tissue-derived stromal cells (ADSCs) are being evaluated for cardiovascular repair. We developed an ex vivo method for producing angiogenic ADSCs transduced with a self-inactivating lentiviral vector (LV) expressing the enhanced green fluorescence protein (EGFP) from an internal cytomegalovirus (CMV) pro...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-012-9537-4
更新日期：2013-05-01 00:00:00
abstract：:Deficiency of the lysosomal glucocerebrosidase (GCR) enzyme results in Gaucher's disease, the most common inherited storage disorder. Treatment consists of enzyme replacement therapy by the administration of recombinant GCR produced in Chinese hamster ovary cells. The production of anti-GCR antibodies has already been...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-010-9303-4
更新日期：2010-11-01 00:00:00
abstract：:Water-soluble quinoprotein glucose dehydrogenase (PQQGDH-B) is a dimeric enzyme whose application for glucose sensing is the focus of much attention. We attempted to increase the thermal stability of PQQGDH-B by introducing a disulfide bond at the dimer interface. The Ser residue at position 415 was selected for subst...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:24:2:97
更新日期：2003-06-01 00:00:00
abstract：:In this study, host-specific forms of the blast pathogen Magnaporthe oryzae in sub-Saharan Africa (SSA) were characterised from distinct cropping locations using a combination of molecular and biological assays. Finger millet blast populations in East Africa revealed a continuous genetic variation pattern and lack of ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-011-9429-z
更新日期：2012-02-01 00:00:00
abstract：:Indirubin is a strong inhibitor of several eukaryotic cell signaling pathways and shows promise as a treatment for myelocytic leukemia and Alzheimer's disease. The tmoABCDEF operon, encoding the components of a novel toluene 4-monooxygenase from the paint factory soil isolate, Pseudomonas sp. M4, was cloned and expres...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-015-9863-4
更新日期：2015-08-01 00:00:00
abstract：:Immobilized-metal-ion affinity chromatography (IMAC) is used extensively for phosphopeptide enrichment in phosphoproteomics. However, the effect of nucleic acids in protein samples on phosphopeptide enrichment by IMAC has not yet been well clarified. In this study, we demonstrate that IMAC beads possess a strong adsor...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-009-9176-6
更新日期：2009-09-01 00:00:00
abstract：:The polyamines spermine, spermidine, and putrescine are small organic molecules one or more of which are present in all living organisms. Many natural products contain polyamine residues. Polyamines are synthesized by a highly regulated pathway from arginine or ornithine and also can be transported in and out of cells...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02788682
更新日期：1999-06-01 00:00:00
abstract：:The Delta12 desaturase represents a diverse gene family in plants and is responsible for conversion of oleic acid (18:1) to linoleic acid (18:2). Several members of this family are known from plants like Arabidopsis and Soybean. Using primers from conserved C- and N-terminal regions, we have cloned a novel Delta12 des...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-009-9150-3
更新日期：2009-06-01 00:00:00
abstract：:Isolation of RNA from recalcitrant tree tissues has been problematic due to large amounts of secondary metabolites and interfering compounds in their cells. We have developed an efficient RNA extraction method, which yielded high-quality RNA preparations from tissues of the lychee tree. The method reported here utiliz...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-007-0073-6
更新日期：2008-01-01 00:00:00
