Abstract:
:Yeast artificial chromosome (YAC) cloning systems enable the cloning of DNA stretches of 50 to well over 2000 kb. This makes it possible to study large intact regions of DNA in detail, by restriction mapping the YAC to produce a physical map and by examining the YAC for coding sequences or genes. YACs are important for their ability to clone the complete sequences of large genes or gene complexes that exceed the size limit for cloning in conventional bacterial cloning vectors like plasmids (up to 10 kb), bacteriophage (15 kb), and cosmids (50 kb). A major advantage of cloning in yeast, a eukaryote, is that many sequences that are unstable, underrepresented, or absent when cloned into prokaryotic systems, remain stable and intact in YAC clones. It is possible to reintroduce YACs intact into mammalian cells where the introduced mammalian genes are expressed and used to study the functions of genes in the context of flanking sequences. The correct protein processing mechanisms are present in the mammalian cells to ensure that a viable protein product is produced.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Ramsay Mdoi
10.1007/BF02921558subject
Has Abstractpub_date
1994-04-01 00:00:00pages
181-201issue
2eissn
1073-6085issn
1559-0305journal_volume
1pub_type
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journal_title:Molecular biotechnology
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pub_type: 杂志文章,收录出版
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:Molecular biotechnology
pub_type: 杂志文章
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更新日期:2003-06-01 00:00:00
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journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
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journal_title:Molecular biotechnology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1995-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-02-01 00:00:00