Yeast artificial chromosome cloning.

Abstract:

:Yeast artificial chromosome (YAC) cloning systems enable the cloning of DNA stretches of 50 to well over 2000 kb. This makes it possible to study large intact regions of DNA in detail, by restriction mapping the YAC to produce a physical map and by examining the YAC for coding sequences or genes. YACs are important for their ability to clone the complete sequences of large genes or gene complexes that exceed the size limit for cloning in conventional bacterial cloning vectors like plasmids (up to 10 kb), bacteriophage (15 kb), and cosmids (50 kb). A major advantage of cloning in yeast, a eukaryote, is that many sequences that are unstable, underrepresented, or absent when cloned into prokaryotic systems, remain stable and intact in YAC clones. It is possible to reintroduce YACs intact into mammalian cells where the introduced mammalian genes are expressed and used to study the functions of genes in the context of flanking sequences. The correct protein processing mechanisms are present in the mammalian cells to ensure that a viable protein product is produced.

journal_name

Mol Biotechnol

journal_title

Molecular biotechnology

authors

Ramsay M

doi

10.1007/BF02921558

subject

Has Abstract

pub_date

1994-04-01 00:00:00

pages

181-201

issue

2

eissn

1073-6085

issn

1559-0305

journal_volume

1

pub_type

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