Abstract:
:Ribosome display is a powerful engineering research tool for the high-throughput selection of peptides or proteins, which results in the generation of high-performance binders against nearly any antigen of interest. As a cell-free display system, ribosome display has been well developed with many outstanding achievements for over 20 years. Compared with other related display techniques, ribosome display shows unique advantages and development prospects. This tool has been successfully exploited for the selection of functional and specific binders in vitro. This review provides a comprehensive survey of the applications of ribosome display in screening or evolving functional proteins as well as in diagnostics and therapeutics. Previous papers on ribosome display failed to comprehensively review evolutionary strategies for proteins. In the present paper, we review all existing evolutionary strategies that have been combined with ribosome display. We also discuss shortcomings, improvement strategies, and research tendency.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Li R,Kang G,Hu M,Huang Hdoi
10.1007/s12033-018-0133-0subject
Has Abstractpub_date
2019-01-01 00:00:00pages
60-71issue
1eissn
1073-6085issn
1559-0305pii
10.1007/s12033-018-0133-0journal_volume
61pub_type
杂志文章,评审abstract::A detailed method is presented for the creation of head-to-tail multimers of short blunt restriction fragments, ligated into a plasmid vector in a single-tube reaction. Random priming of the concatemer insert readily yields hybridization probes of high specificity, unattainable from the short monomer fragments. ...
journal_title:Molecular biotechnology
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abstract::Considering the fact that Prays oleae is one of the most pathogenic insects to the olive tree in the Mediterranean basin, particularly in Tunisia, the mode of action of Cry insecticidal toxins of Bacillus thuringiensis kurstaki in Prays oleae midgut was investigated. The proteolysis of Bacillus thuringiensis delta-end...
journal_title:Molecular biotechnology
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abstract::The macrofungus Cordyceps militaris contains many kinds of bioactive ingredients that are regulated by functional genes, but the functions of many genes in C. militaris are still unknown. In this study, to improve the frequency of homologous integration, a genetic transformation system based on a split-marker approach...
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journal_title:Molecular biotechnology
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journal_title:Molecular biotechnology
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doi:10.1007/s12033-012-9605-9
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journal_title:Molecular biotechnology
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doi:10.1007/s12033-018-0094-3
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journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
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journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF02788682
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journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9476-5
更新日期:2012-09-01 00:00:00
abstract::In this study, host-specific forms of the blast pathogen Magnaporthe oryzae in sub-Saharan Africa (SSA) were characterised from distinct cropping locations using a combination of molecular and biological assays. Finger millet blast populations in East Africa revealed a continuous genetic variation pattern and lack of ...
journal_title:Molecular biotechnology
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doi:10.1007/s12033-011-9429-z
更新日期:2012-02-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1007/BF02752254
更新日期:1997-10-01 00:00:00
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journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0104-5
更新日期:2018-09-01 00:00:00
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journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-017-0015-x
更新日期:2017-07-01 00:00:00
abstract::In the present study, four full-length Dof (DNA-binding with one finger) genes from Sorghum bicolor namely SbDof1, SbDof19, SbDof23, and SbDof24 were PCR amplified, gel eluted, cloned, and sequenced (accession number HQ540084, HQ540085, HQ540086, and HQ540087, respectively). These sequences were further characterized ...
journal_title:Molecular biotechnology
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doi:10.1007/s12033-012-9536-5
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journal_title:Molecular biotechnology
pub_type: 杂志文章
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abstract::We investigated the efficiency of a single plasmid transfection along the longitudinal axis of the regenerating soleus of young rats. This also reflected transfection efficiency along the fibers because the soleus is a nearly fusiform muscle in young animals. The complete regeneration was induced by notexin and the tr...
journal_title:Molecular biotechnology
pub_type: 杂志文章
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journal_title:Molecular biotechnology
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journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
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abstract::Lactococcus lactis is industrially important microorganism used in many dairy fermentations. Numerous genes and gene expression signals from this organism have now been identified and characterized. Recently, several naturally occurring, inducible gene-expression systems have also been described in L. lactis. The main...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
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journal_title:Molecular biotechnology
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journal_title:Molecular biotechnology
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abstract::N-Succinyl-amino acid racemase (NSAAR), long referred to as N-acyl- or N-acetyl-amino acid racemase, is an enolase superfamily member whose biotechnological potential was discovered decades ago, due to its use in the industrial dynamic kinetic resolution methodology first known as "Acylase Process". In previous works,...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-015-9839-4
更新日期:2015-05-01 00:00:00