Abstract：
:Tea, a beverage crop, is a rich source of polyphenols and polysaccharides which greatly attribute to its importance. However, oxidation and precipitation of these compounds during nucleic acids extraction is a limitation to molecular biology and genomic studies. On isolation of total RNA from root tissue using established protocols, difficulties were encountered in terms of purity and quantity of isolated RNA or some of the methods were time-consuming and also yields were low. The present communication combines a phenol-based RNA isolation protocol with a cetyltrimethylammonium bromide-based procedure with appropriate modifications. This protocol successfully isolated RNA from tap root tissue in 2-3 h as compared with 16 h reported by the previous method. Also, RNA yield was higher by more than fourfold. The RNA isolated by this protocol was successfully used for downstream applications such as RT-PCR and the construction of suppression subtractive hybridization library. The developed protocol worked well with other plant tissue with high polyphenols and polysaccharides contents.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Muoki RC,Paul A,Kumari A,Singh K,Kumar Sdoi
10.1007/s12033-011-9476-5subject
Has Abstractpub_date
2012-09-01 00:00:00pages
82-8issue
1eissn
1073-6085issn
1559-0305journal_volume
52pub_type
杂志文章abstract：:The MYB proteins constitute one of the largest transcription factor families in plants. Much research has been performed to determine their structures, functions, and evolution, especially in the model plants, Arabidopsis, and rice. However, this transcription factor family has been much less studied in wheat (Triticu...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-011-9486-3
更新日期：2012-10-01 00:00:00
abstract：:PREP1/p160 is a protein complex with relevant physiopathological roles in vivo. p160 regulates PREP1 transcriptional activity by preventing the formation of other PREP1-containing complexes, whereas PREP1 regulates p160 activity by increasing its stability. This induces the repression of the insulin-regulated glucose ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-016-9932-3
更新日期：2016-05-01 00:00:00
abstract：:Brn-3a, a member of the POU gene family (so-called because of the similarity with the group of transcription factors Pit, Oct, and Unc), was found in neuronal cells engaged in the transcription activity of the p1 and p2 promoters of the most powerful antiapoptotic gene, namely, Bcl-2. The alternative splicing of Brn-3...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:22:2:123
更新日期：2002-10-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02921558
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9786-5
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-015-9888-8
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journal_title：Molecular biotechnology
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:19:1:045
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-020-00245-z
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02686109
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-012-9519-6
更新日期：2013-01-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-018-0092-5
更新日期：2018-08-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-016-9923-4
更新日期：2016-04-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s12033-013-9674-4
更新日期：2013-10-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:15:3:243
更新日期：2000-07-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:29:3:225
更新日期：2005-03-01 00:00:00
abstract：:Human recombinant vascular endothelial growth factor-A121 (hrVEGF-A121) has applications in pharmaceutical industry especially in regenerative medicine. Here, we report the expression, purification, and characterization of hrVEGF-A121 in Escherichia coli expression system using human small ubiquitin-related modifier-3...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-018-0094-3
更新日期：2018-08-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02752699
更新日期：1998-02-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:29:1:57
更新日期：2005-01-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:32:2:093
更新日期：2006-02-01 00:00:00
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journal_title：Molecular biotechnology
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更新日期：2003-11-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-008-9111-2
更新日期：2009-03-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:14:1:01
更新日期：2000-01-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
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更新日期：2001-03-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-007-9024-5
更新日期：2008-05-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9823-4
更新日期：2015-04-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02921609
更新日期：1995-10-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-019-00154-w
更新日期：2019-03-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02907471
更新日期：1995-08-01 00:00:00
abstract：:The REDOX-RESPONSIVE TRANSCRIPTION FACTOR 1 (RRTF1) gene encodes a member of the ERF/AP2 transcription factor family involved in redox homeostasis. The RRTF1 gene shows tissue-specific responsiveness to various abiotic stress treatments including a response to salt stress in roots. An interesting feature of this respo...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-019-00155-9
更新日期：2019-04-01 00:00:00
