Secreted luciferase for in vivo evaluation of systemic protein delivery in mice.

abstract：:An endochitinase gene from the Serratia marcescens Nima strain (chiA Nima) was cloned, sequenced, and expressed in Escherichia coli DH5alphaF', and the recombinant protein (ChiA Nima) was purified by hydrophobic interaction chromatography. chiA Nima contains an open reading frame (ORF) that encodes an endochitinase wi...

journal_title：Molecular biotechnology

authors： Ruiz-Sanchez A,Cruz-Camarillo R,Salcedo-Hernandez R,Ibarra JE,Barboza-Corona JE

更新日期：2005-10-01 00:00:00

abstract：:A detailed method is presented for the creation of head-to-tail multimers of short blunt restriction fragments, ligated into a plasmid vector in a single-tube reaction. Random priming of the concatemer insert readily yields hybridization probes of high specificity, unattainable from the short monomer fragments. ...

journal_title：Molecular biotechnology

authors： Kapros T,Robertson AJ,Waterborg JH

更新日期：1994-08-01 00:00:00

abstract：:DNA damage is one of the most impactful events in living organisms, leading to DNA sequence changes (mutation) and disruption of biological processes. A study has identified a protein called Damage Suppressor Protein (Dsup) in the tardigrade Ramazzotius varieornatus that has shown to reduce the effects of radiation da...

journal_title：Molecular biotechnology

authors： Kirke J,Jin XL,Zhang XH

更新日期：2020-12-01 00:00:00

abstract：:The filamentous fungus Sclerotinia sclerotiorum produces a complete set of cellulolytic enzymes needed for efficient solubilization of native cellulose, the major component of plants. In this work, we reported the molecular characterization of an important glycosyl-hydrolase enzyme classified as endo-β-1,4-glucanase. ...

journal_title：Molecular biotechnology

authors： Chahed H,Ezzine A,Mlouka AB,Hardouin J,Jouenne T,Marzouki MN

更新日期：2014-04-01 00:00:00

abstract：:Classic toxicology studies often utilize in vivo animal models. Newer approaches employing in vitro organ-specific cellular models have been developed in recent years to help accelerate the speed and reduce the cost of traditional toxicology testing. Toward the goal of supporting in vitro cellular model research with ...

journal_title：Molecular biotechnology

authors： Mossoba ME,Vohra SN,Bigley E 3rd,Sprando J,Wiesenfeld PL

更新日期：2020-04-01 00:00:00

abstract：:The aim of this study was to evaluate the usefulness of replicating but non disseminating adenovirus vectors (AdVs) as vaccine vector using human rotavirus (HRV) as a model pathogen. HRV VP7, VP4, or VP4Δ (N-terminal 336 amino acids of VP4) structural proteins as well as the VP4Δ::VP7 chimeric fusion protein were expr...

journal_title：Molecular biotechnology

authors： Girard A,Roques E,St-Louis MC,Massie B,Archambault D

更新日期：2013-07-01 00:00:00

abstract：:Nitrilases have attracted tremendous attention for the preparation of optically pure carboxylic acids. This article aims to address the production and utilization of a highly enantioselective nitrilase from Pseudomonas putida MTCC 5110 for the hydrolysis of racemic mandelonitrile to (R)-mandelic acid. The nitrilase ge...

journal_title：Molecular biotechnology

authors： Banerjee A,Dubey S,Kaul P,Barse B,Piotrowski M,Banerjee UC

更新日期：2009-01-01 00:00:00

abstract：:The REDOX-RESPONSIVE TRANSCRIPTION FACTOR 1 (RRTF1) gene encodes a member of the ERF/AP2 transcription factor family involved in redox homeostasis. The RRTF1 gene shows tissue-specific responsiveness to various abiotic stress treatments including a response to salt stress in roots. An interesting feature of this respo...

journal_title：Molecular biotechnology

authors： Soliman ERS,Meyer P

更新日期：2019-04-01 00:00:00

abstract：:Group B Streptococcus (GBS) is the leading cause of neonatal meningitis and a common pathogen in livestock and aquaculture industries around the world. Conjugate polysaccharide and protein-based vaccines are under development. The surface immunogenic protein (SIP) is a conserved protein in all GBS serotypes and has be...

journal_title：Molecular biotechnology

authors： Díaz-Dinamarca DA,Jerias JI,Soto DA,Soto JA,Díaz NV,Leyton YY,Villegas RA,Kalergis AM,Vásquez AE

更新日期：2018-03-01 00:00:00

abstract：:Isolation of RNA from recalcitrant tree tissues has been problematic due to large amounts of secondary metabolites and interfering compounds in their cells. We have developed an efficient RNA extraction method, which yielded high-quality RNA preparations from tissues of the lychee tree. The method reported here utiliz...

journal_title：Molecular biotechnology

authors： Wang X,Tian W,Li Y

更新日期：2008-01-01 00:00:00

abstract：:Fibrillation of α-synuclein proceeds through distinct stages, with oligomers combining to form the seed or the nucleus, followed by exponential and saturation phases. Osmolytes are considered to act as protein stabilizers by virtue of their ability to inhibit protein aggregation. Trehalose, a non-reducing disaccharide...

journal_title：Molecular biotechnology

authors： Naik V,Kardani J,Roy I

更新日期：2016-04-01 00:00:00

abstract：:Brucellosis, one of the most important re-emerging zoonoses in many countries, is caused by bacteria belonging to the genus Brucella. Furthermore these bacteria represent potential biological warfare agents and the identification of species and biovars of field strains may be crucial for tracing back source of infecti...

journal_title：Molecular biotechnology

authors： De Santis R,Ancora M,De Massis F,Ciammaruconi A,Zilli K,Di Giannatale E,Pittiglio V,Fillo S,Lista F

更新日期：2013-10-01 00:00:00

abstract：:The gram-negative anaerobic oral bacterium Porphyromonas gingivalis initiates periodontal disease through fimbrial attachment to saliva-coated oral surfaces. To study the effects of immunomodulation on enhancement of subunit vaccination, the expression in E. coli and immunogenicity of P. gingivalis fimbrial protein (F...

journal_title：Molecular biotechnology

authors： Kim TG,Huy NX,Kim MY,Jeong DK,Jang YS,Yang MS,Langridge WH,Lee JY

更新日期：2009-02-01 00:00:00

abstract：:The clinical management of immunocompromised patients depends on the rapid identification of infectious agents such as fungal pathogens. The procedure described here for accomplishing this uses a sensitive polymerase chain reaction method, previously reported, combined with restriction-enzyme digestion to distinguish ...

journal_title：Molecular biotechnology

authors： Isik N,White L,Barnes R,Poynton CJ,Mills KI

更新日期：2003-07-01 00:00:00

abstract：:Cardiomyocytes are terminally differentiated cells that to date have been characterized as poor targets for nonviral gene transfer. This study was therefore designed to determine the optimal nonviral gene transfer parameters in cell cultures of neonatal rat cardiomyocytes and to compare them with the efficiency of gen...

journal_title：Molecular biotechnology

authors： Djurovic S,Iversen N,Jeansson S,Hoover F,Christensen G

更新日期：2004-09-01 00:00:00

abstract：:This review outlines the various methods available for submitting sequence data to the EMBL Nucleotide Sequence Database. Depending on the type of sequence data and the facilities available to the submitter, one method may be more suitable than another. Recent developments have been the World Wide Web submission tool,...

journal_title：Molecular biotechnology

authors： Tuli MA,Flores TP,Cameron GN

更新日期：1996-08-01 00:00:00

abstract：:The two asparagine-linked glycosylation sites of recombinant coagulation factor VIIa have been characterized by glycosidase digestions, size-exclusion chromatography (SEC), and mass spectrometry (MS). Nine structures were characterized as core fucosylated bi-and triantennary structures with 0-3 sialic-acid residues wh...

journal_title：Molecular biotechnology

authors： Klausen NK,Bayne S,Palm L

更新日期：1998-06-01 00:00:00

abstract：:While conducting our research on mutations in the human blood platelet glycoprotein Ib-alpha (GPIbalpha) gene, we detected an unusual deletion of 84 bp. This deletion took place in vitro, during PCR and between two direct repeats. It was observed that the deletion could be detected either by the direct sequencing of t...

journal_title：Molecular biotechnology

authors： Hadj Kacem B,Gargouri J,Gargouri A

更新日期：2008-09-01 00:00:00

abstract：:Pyrococcus woesei (DSM 3773) beta-galactosidase gene amplified by polymerase chain reaction was cloned into KpnI and HindIII binding sites of pET-30LIC expression plasmid. The obtained pGal2 (6785 bp) transcription vector was then transferred to Escherichia coli B121 (DE3) cells. High identity (99.9%) of DNA sequences...

journal_title：Molecular biotechnology

authors： Dabrowski S,Maciuńska J,Synowiecki J

更新日期：1998-12-01 00:00:00

abstract：:CD151 is an abundantly expressed eukaryotic transmembrane protein on the cell surface. It is involved in cell adhesion, angiogenesis and signal transduction as well in disease conditions such as cancer and viral infections. However, the molecular mechanism of CD151 activation is poorly understood due to the lack of st...

journal_title：Molecular biotechnology

authors： Purushothaman G,Thiruvenkatam V

更新日期：2019-12-01 00:00:00

abstract：:Compounds based on a flavonoid (di-phenolic) ring structure are emerging as a potentially important new class of pharmaceutical compounds with a broad range of biological activities, most prominent of which are their potential role as anticancer agents. These compounds exert a wide range of upregulating and downregula...

journal_title：Molecular biotechnology

authors： Brown DM,Kelly GE,Husband AJ

更新日期：2005-07-01 00:00:00

abstract：:A gene encoding maltogenic amylase from acidic Bacillus sp. US149 (maUS149) was cloned, sequenced and over-expressed in Escherichia coli. The nucleotide sequence analysis revealed an open reading frame (ORF) of 1749 bp encoding a protein of 582 residues. The alignment of deduced amino acid sequence revealed a relative...

journal_title：Molecular biotechnology

authors： Mabrouk SB,Messaoud EB,Ayadi D,Jemli S,Roy A,Mezghani M,Bejar S

更新日期：2008-03-01 00:00:00

abstract：:Due to the limited number of molecular studies focused on European gene pool investigation, it is necessary to perform plant material recognition. Eighteen accessions of three Miscanthus species, namely, M. × giganteus, M. sinensis, M. sacchariflorus were evaluated with the use of molecular marker systems such as: int...

journal_title：Molecular biotechnology

authors： Cichorz S,Gośka M,Litwiniec A

更新日期：2014-10-01 00:00:00

abstract：:The synthesis of complete genes is becoming a more and more popular approach in heterologous gene expression. Reasons for this are the decreasing prices and the numerous advantages in comparison to classic molecular cloning methods. Two of these advantages are the possibility to adapt the codon usage to the host organ...

journal_title：Molecular biotechnology

authors： Gatter M,Gatter T,Matthäus F

更新日期：2012-10-01 00:00:00

abstract：:Dye-based protein determination assays are widely used to estimate protein concentration, however various reports suggest that the response is dependent on the composition and sequence of the protein, limiting confidence in the resulting concentration estimates. In this study a diverse set of model proteins representi...

journal_title：Molecular biotechnology

authors： Noble JE,Knight AE,Reason AJ,Di Matola A,Bailey MJ

更新日期：2007-10-01 00:00:00

abstract：:Despite the known importance of the composition of culture media and culture conditions on Bacillus thuringiensis growth and toxicity, very few reviews are concerned with this subject. This article reviews some aspects of the microbiology of Bacillus thuringiensis, and how toxicity is affected by the composition of gr...

journal_title：Molecular biotechnology

authors： Avignone-Rossa C,Mignone CF

更新日期：1995-08-01 00:00:00

abstract：:HOX genes are highly conserved transcription factors that determine the identity of cells and tissues along the anterior-posterior body axis in developing embryos. Aberrations in HOX gene expression have been shown in various tumors. However, the correlation of HOX gene expression patterns with tumorigenesis and cance...

journal_title：Molecular biotechnology

authors： Hur H,Lee JY,Yun HJ,Park BW,Kim MH

更新日期：2014-01-01 00:00:00

abstract：:Monoclonal antibodies (Mabs) against human chorionic gonadotropin hormone (hCG) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. Sixty-five percent of the total culture wells exhibited hybrid growth and 8% of the total wells (13 culture wells) contained anti-hCG secreting hybrids. A pos...

journal_title：Molecular biotechnology

authors： Novo C,Domingos A,Karmali A

更新日期：2001-02-01 00:00:00

abstract：:Amplification of DNA or RNA sequences using the polymerase chain reaction (PCR) or reverse transcriptase PCR (RT-PCR) requires primers of an appropriate length to be designed. Two hexamer restriction primers, denoted as E101 and H301, which correspond to sequences of EcoRI and HindIII recognition sites, respectively, ...

journal_title：Molecular biotechnology

authors： Ryu KH,Choi SH,Lee JS

更新日期：2000-01-01 00:00:00

abstract：:Low molecular weight RNA (LMW RNA) is generally obtained either from the total RNA or from total nucleic acids solution. Many steps and chemical reagents are involved in traditional methods for LMW RNA isolation where degradation of LMW RNA often occurs, especially for plant materials with high levels of secondary cat...

journal_title：Molecular biotechnology

authors： Wang XW,Xiong AS,Yao QH,Zhang Z,Qiao YS

更新日期：2010-01-01 00:00:00