Abstract:
:Addition of taxol into suspension cultures of Taxus cuspidata induced cell apoptosis, which was confirmed by gel electrophoresis of the DNA ladders indicating the progressive delineation of fragmented nuclear DNA (nDNA) into distinct bodies. The additional taxol not only changed the microtubule assembly of cells, but also affected the gene expression. Fourteen cDNA fragments, named as TIGT9-22, were isolated after addition of taxol and their GenBank accession numbers were given as BF704560-BF704573, respectively. Among them, TIGT13 and TIGT21 were apparently homogeneous with apbE and carbamoylphosphate synthetase, respectively. Other cDNA fragments showed no significant analogy with the known sequences in GenBank.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Yuan YJ,Ma ZY,Wu JCdoi
10.1385/MB:20:2:137keywords:
subject
Has Abstractpub_date
2002-02-01 00:00:00pages
137-43issue
2eissn
1073-6085issn
1559-0305pii
MB:20:2:137journal_volume
20pub_type
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