Abstract:
:The enzyme avian myeloblastosis virus reverse transcriptase (AMV-RT) is routinely used for cDNA synthesis, which is generally carried out at temperatures between 37 degrees C and 42 degrees C. We show that this enzyme can support cDNA synthesis, at temperatures as high as 70 degrees C. We have utilized this property of the AMV-RT to improve the specificity of polymerase chain reaction (PCR). Furthermore, this apparently thermophilic property of the enzyme, which is an important constituent of a mesophilic organism, raises intriguing questions regarding evolution of the enzyme structure.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Fuchs B,Zhang K,Rock MG,Bolander ME,Sarkar Gdoi
10.1385/MB:12:3:237keywords:
subject
Has Abstractpub_date
1999-10-01 00:00:00pages
237-40issue
3eissn
1073-6085issn
1559-0305pii
MB:12:3:237journal_volume
12pub_type
杂志文章abstract::Costimulatory signal(s) in addition to engagement of T cell receptor is important for optimal activation of T lymphocytes. During last decade several dozens of new costimulatory molecules, including 4-1BB, CD40, OX40, CD27, and ICOS, have been identified. It is likely that in Post-Genome Era more molecules with potent...
journal_title:Molecular biotechnology
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doi:10.1385/MB:21:3:259
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doi:10.1007/s12033-020-00281-9
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abstract::Recombinant adenovirus vectors have proven to be useful tools in facilitating gene transfer. Construction of such vectors requires a knowledge of the adenovirus genome structure and its life cycle. A commonly used recombinant adenovirus involves deletion of the E1 region; such a recombinant is traditionally produced b...
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doi:10.1385/MB:25:3:275
更新日期:2003-11-01 00:00:00
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journal_title:Molecular biotechnology
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abstract::Megaprimer-based methodology has been widely applied in site-directed mutagenesis, but rarely used in gene splicing. In this article, we describe a modification of the megaprimer PCR method, which can efficiently create and amplify a specific ligated chimeric gene segment in a PCR reaction and under a common PCR progr...
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abstract::The concept of photoprotection by dietary means is gaining momentum. Plant constituents such as carotenoids and flavonoids are involved in protection against excess light in plants and contribute to the prevention of UV damage in humans. As micronutrients, they are ingested with the diet and are distributed into light...
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journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-016-9932-3
更新日期:2016-05-01 00:00:00
abstract::The extracellular signal-regulated kinase (ERK) cascade is a central intracellular signaling pathway that is activated by a variety of extracellular stimuli, and thereby regulates cellular processes such as proliferation, differentiation, and oncogenic transformation. To execute these functions, the signals of those s...
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journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02760858
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abstract::Cardiomyocytes are terminally differentiated cells that to date have been characterized as poor targets for nonviral gene transfer. This study was therefore designed to determine the optimal nonviral gene transfer parameters in cell cultures of neonatal rat cardiomyocytes and to compare them with the efficiency of gen...
journal_title:Molecular biotechnology
pub_type: 杂志文章
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更新日期:2004-09-01 00:00:00
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journal_title:Molecular biotechnology
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journal_title:Molecular biotechnology
pub_type: 杂志文章
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更新日期:2007-10-01 00:00:00
abstract::The induction of several secondary metabolites in plants is one of the most commonly observed effects after the external addition of methyl jasmonate (MeJA). After the elicitation of Catharanthus roseus hairy roots with different concentrations of MeJA, changes in the accumulation of alkaloids such as ajmalicine, serp...
journal_title:Molecular biotechnology
pub_type: 杂志文章
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更新日期:2009-03-01 00:00:00
abstract::Production of monoclonal antibodies and pharmaceutical proteins in transgenic plants has been the focus of many research efforts for close to 30 years. Use of plants as bioreactors reduces large-scale production costs and minimizes risk for human pathogens contamination. Stable nuclear transformation of the plant geno...
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pub_type: 杂志文章
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journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-010-9303-4
更新日期:2010-11-01 00:00:00
abstract::Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals reflecting their diverse applications in research and the clinic. The correct glycosylation of mAbs is required to elicit effector functions such as complement-dependent and antibody-dependent cell-mediated cytotoxicity, although these ma...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9531-x
更新日期:2013-03-01 00:00:00
abstract::Virus crystallography can provide atomic resolution structures for intact isometric virus particles and components thereof. The methodology is illustrated by reference to a particularly complex example, the core of the bluetongue virus (700 A). ...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:12:1:13
更新日期:1999-08-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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更新日期:2007-01-01 00:00:00
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journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02740771
更新日期:1996-10-01 00:00:00
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journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-008-9135-7
更新日期:2009-05-01 00:00:00
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journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
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更新日期:2011-10-01 00:00:00
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journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:17:2:119
更新日期:2001-02-01 00:00:00
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journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-013-9698-9
更新日期:2014-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s12033-011-9393-7
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journal_title:Molecular biotechnology
pub_type: 杂志文章
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更新日期:1994-08-01 00:00:00
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journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02686109
更新日期:2007-02-01 00:00:00