Abstract：
:Since it was first introduced in late 1990s Wave bioreactor has been used for protein production by mammalian and insect cell lines. However, using Wave bioreactor to produce human monoclonal antibody by stable Drosophila Schneider 2 (S2) cell transfectants has not been reported before. In this study, S2 cells were co-transfected with an inducible vector expressing human monoclonal antibody heavy and light chains, respectively, specific for hemagglutinin (HA) of H5N1 influenza virus. Stable S2 transfectant clone was selected by limiting dilution assay. Stable S2 transfectant clone that produce the highest amount of human monoclonal antibody was inoculated into two 2-l disposable cellbags, where cell growth and antibody production were compared between batch and perfusion cultures using Wave bioreactor. Here, we report that maximum viable cell density reached 1.06 × 10(7) cells/ml in batch culture; whereas 1.04 × 10(8) cells/ml was achieved in perfusion culture. The maximum volumetric antibody productivity in batch culture was 52 mg/l/day; while perfusion culture yielded 1,437 mg/l/day. As a result, the total antibody production was 201 mg in batch culture and 8,212 mg in perfusion culture. The antibody produced by both cultures displays full neutralizing activity. Thus, our results provide strong support for using Wave bioreactor in perfusion culture for a large-scale production of human monoclonal antibody by stable S2 cell transfectants.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Wang L,Hu H,Yang J,Wang F,Kaisermayer C,Zhou Pdoi
10.1007/s12033-011-9484-5subject
Has Abstractpub_date
2012-10-01 00:00:00pages
170-9issue
2eissn
1073-6085issn
1559-0305journal_volume
52pub_type
杂志文章abstract：:IMGT, the International ImMunoGeneTics information system ( http://imgt.cines.fr ), was created in 1989 by the Laboratoire d'ImmunoGénétique Moléculaire (LIGM) (Université Montpellier 2 and CNRS) at Montpellier, France, in order to standardize and manage the complexity of immunogenetics data. IMGT is recognized as the...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-008-9062-7
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-007-9007-6
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02760814
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journal_title：Molecular biotechnology
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journal_title：Molecular biotechnology
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doi：10.1007/BF02789291
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更新日期：2014-11-01 00:00:00
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更新日期：2012-10-01 00:00:00
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更新日期：2017-07-01 00:00:00
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pub_type： 杂志文章
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更新日期：2014-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s12033-011-9476-5
更新日期：2012-09-01 00:00:00
abstract：:D-Glucosamine is a commonly used dietary supplement that promotes cartilage health in humans. Metabolic flux analysis showed that D-glucosamine production could be increased by blocking three pathways involved in the consumption of glucosamine-6-phosphate and acetylglucosamine-6-phosphate. By homologous single-exchang...
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journal_title：Molecular biotechnology
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journal_title：Molecular biotechnology
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journal_title：Molecular biotechnology
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s12033-011-9402-x
更新日期：2011-11-01 00:00:00
