Molecular Cloning of an Amino Acid Permease Gene and Structural Characterization of the Protein in Common Bean (Phaseolus vulgaris L.).

abstract：:D-Glucosamine is a commonly used dietary supplement that promotes cartilage health in humans. Metabolic flux analysis showed that D-glucosamine production could be increased by blocking three pathways involved in the consumption of glucosamine-6-phosphate and acetylglucosamine-6-phosphate. By homologous single-exchang...

journal_title：Molecular biotechnology

authors： Li P,Li X,Wen Z,Dong R,Li K,Fan H,Wang R,Wang J,Lv M

更新日期：2020-08-01 00:00:00

abstract：:Papaya ringspot virus (PRSV) causes severe economic losses in both cucurbits and papaya throughout the tropics and subtropics. Development of PRSV-resistant transgenic plants faces a major hurdle in achieving resistance against geographically distinct isolates. One of the major reasons of failing to achieve the broad-...

journal_title：Molecular biotechnology

authors： Mangrauthia SK,Singh P,Praveen S

更新日期：2010-01-01 00:00:00

abstract：:ATP-binding cassette transporter G2 (ABCG2) gene encodes a protein that has a wide variety of substrates and is responsible for the active secretion of clinically and toxicologically important molecules into milk. Although known in many species, this marks the first time this gene product has been reported in goats. I...

journal_title：Molecular biotechnology

authors： Wu HJ,Luo J,Wu N,Matand K,Zhang LJ,Han XF,Yang BJ

更新日期：2008-05-01 00:00:00

abstract：:Classic toxicology studies often utilize in vivo animal models. Newer approaches employing in vitro organ-specific cellular models have been developed in recent years to help accelerate the speed and reduce the cost of traditional toxicology testing. Toward the goal of supporting in vitro cellular model research with ...

journal_title：Molecular biotechnology

authors： Mossoba ME,Vohra SN,Bigley E 3rd,Sprando J,Wiesenfeld PL

更新日期：2020-04-01 00:00:00

abstract：:Monoclonal antibodies (Mabs) against human chorionic gonadotropin hormone (hCG) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. Sixty-five percent of the total culture wells exhibited hybrid growth and 8% of the total wells (13 culture wells) contained anti-hCG secreting hybrids. A pos...

journal_title：Molecular biotechnology

authors： Novo C,Domingos A,Karmali A

更新日期：2001-02-01 00:00:00

abstract：:Podophyllotoxin (PPT) is a plant natural product that serves as a precursor for the synthesis of many well-known chemotherapeutic drugs. The limited availability and high demand for the source plants of PPT have led to the exploration of alternative sources for this compound. In this study, we utilized the endophytic ...

journal_title：Molecular biotechnology

authors： Arneaud SL,Porter JR

更新日期：2015-12-01 00:00:00

abstract：:Efficient display of antibody on filamentous phage M13 coat is crucial for successful biopanning selections. We applied a directed evolution strategy to improve the oligovalent display of a poorly behaving Fab fragment fused to phage gene-3 for minor coat protein (g3p). The Fab displaying clones were enriched from a r...

journal_title：Molecular biotechnology

authors： Huovinen T,Sanmark H,Ylä-Pelto J,Vehniäinen M,Lamminmäki U

更新日期：2010-03-01 00:00:00

abstract：:Malaria is a serious but preventable and treatable infectious disease that is found in over 100 countries around the world. Correct and rapid diagnosis of malaria infection can rescue the patient of getting sicker and reduces the risk of disease spreading among humans. Chlamydomonas reinhardtii chloroplast is an attra...

journal_title：Molecular biotechnology

authors： Shamriz S,Ofoghi H

更新日期：2019-02-01 00:00:00

abstract：:Enzymes isolated from extremophiles often exhibit superior performance and potential industrial applications. There are several advantages performing biocatalysis at elevated temperatures, including enhanced reaction rates, increased substrate solubility and decreased risks of contamination. Furthermore, thermophilic ...

journal_title：Molecular biotechnology

authors： Mandelli F,Gonçalves TA,Gandin CA,Oliveira AC,Oliveira Neto M,Squina FM

更新日期：2016-11-01 00:00:00

abstract：:The ability to identify disease-associated genes using microarray technology is dependent on isolating high-quality total RNA from the diseased tissue under study. However, it is not always possible to obtain large amounts of affected tissue from patients and therefore the quality and yield of RNA may be compromised. ...

journal_title：Molecular biotechnology

authors： Murray EJ

更新日期：2003-02-01 00:00:00

abstract：:Cardiomyocytes are terminally differentiated cells that to date have been characterized as poor targets for nonviral gene transfer. This study was therefore designed to determine the optimal nonviral gene transfer parameters in cell cultures of neonatal rat cardiomyocytes and to compare them with the efficiency of gen...

journal_title：Molecular biotechnology

authors： Djurovic S,Iversen N,Jeansson S,Hoover F,Christensen G

更新日期：2004-09-01 00:00:00

abstract：:In this study, human alpha-1,4-N-acetylglucosaminyltransferase (alpha4GnT) fused with GFP(uv) (GFP(uv)-alpha4GnT) was expressed using both a transformed cell system and silkworm larvae. A Tn-pXgp-GFP(uv)-alpha4GnT cell line, isolated after expression vector transfection, produced 106 mU/ml of alpha4GnT activity in sus...

journal_title：Molecular biotechnology

authors： Nakajima M,Kato T,Kanamasa S,Park EY

更新日期：2009-09-01 00:00:00

abstract：:The filamentous fungus Sclerotinia sclerotiorum produces a complete set of cellulolytic enzymes needed for efficient solubilization of native cellulose, the major component of plants. In this work, we reported the molecular characterization of an important glycosyl-hydrolase enzyme classified as endo-β-1,4-glucanase. ...

journal_title：Molecular biotechnology

authors： Chahed H,Ezzine A,Mlouka AB,Hardouin J,Jouenne T,Marzouki MN

更新日期：2014-04-01 00:00:00

abstract：:The Delta12 desaturase represents a diverse gene family in plants and is responsible for conversion of oleic acid (18:1) to linoleic acid (18:2). Several members of this family are known from plants like Arabidopsis and Soybean. Using primers from conserved C- and N-terminal regions, we have cloned a novel Delta12 des...

journal_title：Molecular biotechnology

authors： Khadake RM,Ranjekar PK,Harsulkar AM

更新日期：2009-06-01 00:00:00

abstract：:DNA beta is an approx 1350 nucleotide, single-stranded DNA molecule which has been shown to be associated with some monopartite geminiviruses of the genus Begomovirus. This component requires the helper begomovirus for replication in the cells of host plants and for insect transmission, possibly by trans-encapsidation...

journal_title：Molecular biotechnology

authors： Briddon RW,Bull SE,Mansoor S,Amin I,Markham PG

更新日期：2002-03-01 00:00:00

abstract：:The electron microscopic study of DNA-protein complexes can yield valuable information that is often not easily available by other methods. In this article we give a number of examples that were chosen to illustrate the utility of electron microscopy. Along with the strategy used are protocols that allow such experime...

journal_title：Molecular biotechnology

authors： Schnos M,Inman RB

更新日期：2000-09-01 00:00:00

abstract：:The proteasome is a multi-subunit protein complex that serves as a major pathway for intracellular protein degradation, playing important functions in various biological processes. The C-terminus of the β7 (PSMB4) proteasome subunit was tagged with EGFP and with a composite element for affinity purification and TEV cl...

journal_title：Molecular biotechnology

authors： Kulichkova VA,Artamonova TO,Zaykova JJ,Ermolaeva JB,Khodorkovskii MA,Barlev NA,Tomilin AN,Tsimokha AS

更新日期：2015-01-01 00:00:00

abstract：:A shuttle vector pZL1 which can replicate both in Gluconobacter oxydans and Escherichia coli was constructed based on G. oxydans DSM2003 cryptic plasmid pGOX3, a homology of G. oxydans 621H pGOX3, and E. coli cloning vector pUC18. It was found to be stably maintained in G. oxydans during the serial subcultures in the ...

journal_title：Molecular biotechnology

authors： Zhang L,Lin J,Ma Y,Wei D,Sun M

更新日期：2010-11-01 00:00:00

abstract：:Translation initiation of Coxsackievirus B3 (CVB3) RNA is directed by an internal ribosome entry site (IRES) within the 5' untranslated region. Host cell factors involved in this process include some canonical translation factors and additional RNA-binding proteins. We have, previously, described that the Sabin3-like ...

journal_title：Molecular biotechnology

authors： Souii A,M'hadheb-Gharbi MB,Aouni M,Gharbi J

更新日期：2013-06-01 00:00:00

abstract：:Adeno-associated virus (AAV) is a small, non-enveloped virus used as vector in gene therapy, mainly produced in human cells and in baculovirus systems. Intense studies on these platforms led to the production of vectors with titers between 103 and 105 viral genomes (vg) per cells. In spite of this, vector yields need ...

journal_title：Molecular biotechnology

authors： Galli A,Iaia I,Milella MS,Cipriani F,Della Latta V,Giacca M,Zentilin L,Cervelli T

更新日期：2021-02-01 00:00:00

abstract：:The synthesis of complete genes is becoming a more and more popular approach in heterologous gene expression. Reasons for this are the decreasing prices and the numerous advantages in comparison to classic molecular cloning methods. Two of these advantages are the possibility to adapt the codon usage to the host organ...

journal_title：Molecular biotechnology

authors： Gatter M,Gatter T,Matthäus F

更新日期：2012-10-01 00:00:00

abstract：:The extracellular signal-regulated kinase (ERK) cascade is a central intracellular signaling pathway that is activated by a variety of extracellular stimuli, and thereby regulates cellular processes such as proliferation, differentiation, and oncogenic transformation. To execute these functions, the signals of those s...

journal_title：Molecular biotechnology

authors： Chuderland D,Seger R

更新日期：2005-01-01 00:00:00

abstract：:We report a rapid and simple method for isolating the 5'-end of plant genes from genomic DNA by polymerase chain reaction (PCR) with TATA-box-based degenerate primers (TDPs). The TDPs were specially designed according to the TATA box, which is conserved in the promoter region of most plant genes. The unknown 5'-ends o...

journal_title：Molecular biotechnology

authors： Guo Y,Ma L,Ji Y,Pu G,Liu B,Du Z,Li G,Ye H,Wang H

更新日期：2011-02-01 00:00:00

abstract：:PREP1/p160 is a protein complex with relevant physiopathological roles in vivo. p160 regulates PREP1 transcriptional activity by preventing the formation of other PREP1-containing complexes, whereas PREP1 regulates p160 activity by increasing its stability. This induces the repression of the insulin-regulated glucose ...

journal_title：Molecular biotechnology

authors： Lorenzo V,Mascanzoni F,Vitagliano L,Ruvo M,Doti N

更新日期：2016-05-01 00:00:00

abstract：:Lactic acid bacteria (LAB) are functional starter cultures in food and dairy industry and are also regarded as power houses for bioprocess and fermentation technology. Due to extensive applications in food and medical applications, intensive research and developmental activities are going on throughout the world to un...

journal_title：Molecular biotechnology

authors： Vivek N,Hazeena SH,Rajesh RO,Godan TK,Anjali KB,Nair LM,Mohan B,Nair SC,Sindhu R,Pandey A,Binod P

更新日期：2019-08-01 00:00:00

abstract：:In this paper, we report cloning of a pectate lyase gene from Bacillus amyloliquefaciens S6 (pelS6), and biochemical characterization of the recombinant pectate lyase. PelS6 was found to be identical with B. subtilis 168 pel enzyme with 100% amino acid sequence homology. Although these two are genetically very close, ...

journal_title：Molecular biotechnology

authors： Bekli S,Aktas B,Gencer D,Aslim B

更新日期：2019-09-01 00:00:00

abstract：:NK cells have been seen as potential agents in adoptive immunotherapy for cancer. The main challenge for the success of this approach is to obtain a great quantity of activated NK cells for adoptive transfer. The present study had aimed to evaluate the effect of a feeder layer of irradiated MSCs in the in vitro expans...

journal_title：Molecular biotechnology

authors： Pedroso JF,de Souza Valim V,Pezzi A,Furlan JM,Lenhart G,Sehn F,Zambonato B,Gonçalves AD,Wilke I,Amorin B,da Silva MA,Pedrazzani FS,da Rocha Silla LM

更新日期：2020-05-01 00:00:00

abstract：:A synthetic gene encoding a single chain Fv fragment of an antibody directed against the nuclear inclusion a (NIa) protein of potato virus Y (PVY) was used to transform two commercial potato cultivars (Claustar and BF15). The NIa protease forms the nuclear inclusion body A and acts as the major protease in the cleavag...

journal_title：Molecular biotechnology

authors： Gargouri-Bouzid R,Jaoua L,Rouis S,Saïdi MN,Bouaziz D,Ellouz R

更新日期：2006-06-01 00:00:00

abstract：:Lentivirus-mediated RNA interference (RNAi) is a potent experimental tool for investigating gene functions in vitro and in vivo. It has advantages that transgenic technology lacks. However, in vivo applications are difficult to apply in the central nervous system of non-model organisms due to the lack of a standard br...

journal_title：Molecular biotechnology

authors： Chen Q,Zhu T,Jones G,Zhang J,Sun Y

更新日期：2013-06-01 00:00:00

abstract：:The aim of this study was to evaluate the usefulness of replicating but non disseminating adenovirus vectors (AdVs) as vaccine vector using human rotavirus (HRV) as a model pathogen. HRV VP7, VP4, or VP4Δ (N-terminal 336 amino acids of VP4) structural proteins as well as the VP4Δ::VP7 chimeric fusion protein were expr...

journal_title：Molecular biotechnology

authors： Girard A,Roques E,St-Louis MC,Massie B,Archambault D

更新日期：2013-07-01 00:00:00