Abstract：
:Human immunodeficiency virus types 1 and 2 (HIV-1, -2), the etiological agents of AIDS, are retroviruses that replicate in CD4+ T lymphocytes, monocytes, and macrophages. Early anti-HIV therapies were directed at the step in the virus life cycle that was considered the most readily blocked: the transcription of viral RNA into its DNA copy by the viral enzyme reverse transcriptase (RT). Unfortunately, to date, patient therapies have been relatively unsuccessful and hampered by toxicological problems. This has promoted further research into inhibitors of HIV replication, which may act at alternative stages in the viral replicative cycle as well as more effective RT inhibitors. In order to facilitate such research, simple and accurate in vitro assays are highly desirable. Here we describe such assays that measure components of the HIV replicative cycle and are suitable for use within antiviral experiments.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Patience C,Moore J,Boyd Mdoi
10.1007/BF02821510subject
Has Abstractpub_date
1994-02-01 00:00:00pages
49-58issue
1eissn
1073-6085issn
1559-0305journal_volume
1pub_type
杂志文章abstract：:Lactic acid bacteria (LAB) are functional starter cultures in food and dairy industry and are also regarded as power houses for bioprocess and fermentation technology. Due to extensive applications in food and medical applications, intensive research and developmental activities are going on throughout the world to un...
journal_title：Molecular biotechnology
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pub_type： 杂志文章
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journal_title：Molecular biotechnology
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journal_title：Molecular biotechnology
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journal_title：Molecular biotechnology
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doi：10.1007/s12033-010-9260-y
更新日期：2010-06-01 00:00:00
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journal_title：Molecular biotechnology
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9756-y
更新日期：2014-08-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02740817
更新日期：1997-06-01 00:00:00
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pub_type： 杂志文章
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doi：10.1385/MB:33:3:199
更新日期：2006-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s12033-012-9546-3
更新日期：2013-05-01 00:00:00
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pub_type： 杂志文章,评审
doi：10.1007/s12033-018-0133-0
更新日期：2019-01-01 00:00:00
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journal_title：Molecular biotechnology
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
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更新日期：2005-07-01 00:00:00
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journal_title：Molecular biotechnology
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更新日期：2005-09-01 00:00:00
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journal_title：Molecular biotechnology
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journal_title：Molecular biotechnology
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9731-7
更新日期：2014-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF02740841
更新日期：1998-12-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-016-9923-4
更新日期：2016-04-01 00:00:00
abstract：:A detailed method is presented for the creation of head-to-tail multimers of short blunt restriction fragments, ligated into a plasmid vector in a single-tube reaction. Random priming of the concatemer insert readily yields hybridization probes of high specificity, unattainable from the short monomer fragments. ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02789291
更新日期：1994-08-01 00:00:00
