Secretory expression and purification of recombinant Escherichia coli heat-labile enterotoxin B subunit and its applications on intranasal vaccination of hantavirus.

abstract：:High-quality RNA preparations are critical for further applications such as reverse transcriptase-polymerase chain reaction (RT-PCR) transcript amplifications, and elaboration of cDNA and expressed sequence tag libraries. Melanins are phenolic compounds present in many fungi and apparently play key roles in fungi path...

journal_title：Molecular biotechnology

authors： Islas-Flores I,Peraza-Echeverría L,Canto-Canché B,Rodríguez-García CM

更新日期：2006-09-01 00:00:00

abstract：:Compounds based on a flavonoid (di-phenolic) ring structure are emerging as a potentially important new class of pharmaceutical compounds with a broad range of biological activities, most prominent of which are their potential role as anticancer agents. These compounds exert a wide range of upregulating and downregula...

journal_title：Molecular biotechnology

authors： Brown DM,Kelly GE,Husband AJ

更新日期：2005-07-01 00:00:00

abstract：:In this article, four types of standards were assessed in a SYBR Green-based real-time PCR procedure for the quantification of Staphylococcus aureus (S. aureus) in DNA samples. The standards were purified S. aureus genomic DNA (type A), circular plasmid DNA containing a thermonuclease (nuc) gene fragment (type B), DNA...

journal_title：Molecular biotechnology

authors： Martinon A,Cronin UP,Wilkinson MG

更新日期：2012-01-01 00:00:00

abstract：:Positional cloning of rare disease genes depends on the availability of highly polymorphic markers near the disease loci. The most abundant class of polymorphic markers in the human genome is CA-repeats. We have developed a strategy for the rapid isolation of highly polymorphic CA-repeats from YAC clones. Total DNA of...

journal_title：Molecular biotechnology

authors： Rotman G,Vanagaite L,Collins FS,Shiloh Y

更新日期：1995-04-01 00:00:00

abstract：:In the present study, four full-length Dof (DNA-binding with one finger) genes from Sorghum bicolor namely SbDof1, SbDof19, SbDof23, and SbDof24 were PCR amplified, gel eluted, cloned, and sequenced (accession number HQ540084, HQ540085, HQ540086, and HQ540087, respectively). These sequences were further characterized ...

journal_title：Molecular biotechnology

authors： Kushwaha H,Gupta S,Singh VK,Bisht NC,Sarangi BK,Yadav D

更新日期：2013-05-01 00:00:00

abstract：:We report on a unified rapid betaine-based-PCR protocol for amplification of the (CAG)n region in Huntington disease (HD) and the (CGG)n region in Fragile X syndrome (FXS), followed by an electrophoretic separation on automated sequencer for precise determination of the triplet numbers. The high betaine concentration ...

journal_title：Molecular biotechnology

authors： Todorov T,Todorova A,Georgieva B,Mitev V

更新日期：2010-06-01 00:00:00

abstract：:Artificial microRNA (amiRNA) has become a powerful tool for gene silencing in plants. A new method for easy and rapid construction of rice artificial miRNA vector is described. The procedure involved modification of the pCAMBIA1300-UR vector by insertion of a 'vector modification fragment'. This was prepared from the ...

journal_title：Molecular biotechnology

authors： Wang X,Yang Y,Yu C,Zhou J,Cheng Y,Yan C,Chen J

更新日期：2010-11-01 00:00:00

abstract：:Dye-based protein determination assays are widely used to estimate protein concentration, however various reports suggest that the response is dependent on the composition and sequence of the protein, limiting confidence in the resulting concentration estimates. In this study a diverse set of model proteins representi...

journal_title：Molecular biotechnology

authors： Noble JE,Knight AE,Reason AJ,Di Matola A,Bailey MJ

更新日期：2007-10-01 00:00:00

abstract：:Technological achievements in the last 5 to 10 yr and their application to sequencing and polymorphism discovery in the human genome have fostered a renewed interest in the genetic basis of drug response. Consequently, the field of pharmacogenetics/pharmacogenomics has been gaining momentum, fueled not only on technol...

journal_title：Molecular biotechnology

authors： McCarthy JJ

更新日期：2003-11-01 00:00:00

abstract：:Low molecular weight RNA (LMW RNA) is generally obtained either from the total RNA or from total nucleic acids solution. Many steps and chemical reagents are involved in traditional methods for LMW RNA isolation where degradation of LMW RNA often occurs, especially for plant materials with high levels of secondary cat...

journal_title：Molecular biotechnology

authors： Wang XW,Xiong AS,Yao QH,Zhang Z,Qiao YS

更新日期：2010-01-01 00:00:00

abstract：:This article reviews some of the issues involved in evaluating vaccines in humans. Vaccine trials are required for licensure and are essential for demonstrating a vaccine's safety and protective efficacy. The formal framework of phase I, II, and III trials is described, with particular emphasis on the choice of hypoth...

journal_title：Molecular biotechnology

authors： Farrington CP,Miller E

更新日期：2001-01-01 00:00:00

abstract：:Modern sugarcane cultivars have complex genetic characteristics and low fertility that render their genetic improvement through traditional breeding difficult. Genetic engineering methodology to introduce foreign genes provides new opportunities for the genetic improvement of sugarcane cultivars. One of prerequisites ...

journal_title：Molecular biotechnology

authors： Santosa DA,Hendroko R,Farouk A,Greiner R

更新日期：2004-10-01 00:00:00

abstract：:Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals reflecting their diverse applications in research and the clinic. The correct glycosylation of mAbs is required to elicit effector functions such as complement-dependent and antibody-dependent cell-mediated cytotoxicity, although these ma...

journal_title：Molecular biotechnology

authors： Hristodorov D,Fischer R,Joerissen H,Müller-Tiemann B,Apeler H,Linden L

更新日期：2013-03-01 00:00:00

abstract：:The polyamines spermine, spermidine, and putrescine are small organic molecules one or more of which are present in all living organisms. Many natural products contain polyamine residues. Polyamines are synthesized by a highly regulated pathway from arginine or ornithine and also can be transported in and out of cells...

journal_title：Molecular biotechnology

authors： Morgan DM

更新日期：1999-06-01 00:00:00

abstract：:Characterization of novel plant gene promoters underpins the development of transgenic crop plants. Here, we report a novel 5' regulatory sequence (Eriubi D7) of the ubiquitin gene from Erianthus arundinaceus, a wild relative of sugarcane resistant to many biotic and abiotic stresses. A 3.2-kb regulatory sequence of u...

journal_title：Molecular biotechnology

authors： Chakravarthi M,Philip A,Subramonian N

更新日期：2015-09-01 00:00:00

abstract：:Ribosome display is a powerful engineering research tool for the high-throughput selection of peptides or proteins, which results in the generation of high-performance binders against nearly any antigen of interest. As a cell-free display system, ribosome display has been well developed with many outstanding achieveme...

journal_title：Molecular biotechnology

authors： Li R,Kang G,Hu M,Huang H

更新日期：2019-01-01 00:00:00

abstract：:Laccases are polyphenol oxidoreductases used in a number of industrial applications. Due to the increasing demand for these "green catalysis" enzymes, the identification and biochemical characterisation of their novel properties is essential. In our study, cloned Madurella mycetomatis laccase (mmlac) genes were hetero...

journal_title：Molecular biotechnology

authors： Tülek A,Karataş E,Çakar MM,Aydın D,Yılmazcan Ö,Binay B

更新日期：2021-01-01 00:00:00

abstract：:Adenoviral dodecahedron (Dd) is a virus-like particle composed of twelve pentameric penton base (Pb) proteins, responsible for adenovirus cell penetration. It is generated spontaneously in the baculovirus system upon expression of the Pb gene of adenovirus serotype 3. This particle shows remarkable cell penetration ab...

journal_title：Molecular biotechnology

authors： Szurgot I,Jedynak M,Podsiadla-Bialoskorska M,Piwowarski J,Szolajska E,Chroboczek J

更新日期：2015-06-01 00:00:00

abstract：:Previous studies have shown that mRNA-electroporated dendritic cells (DCs) are able to process and present tumor-associated antigens, leading to the activation of tumor-specific T cells in vitro and in vivo. However, the optimal maturation state of antigen loading and half-life of the mRNA-translated protein product a...

journal_title：Molecular biotechnology

authors： Met O,Eriksen J,Svane IM

更新日期：2008-10-01 00:00:00

abstract：:Surface plasmon resonance (SPR) biosensor is a promising technology for its various advantages including the real-time measurement of biomolecular interactions without labeling. A method of hybridizing RNAs on the surface of the streptavidin-coated (SA) sensor chip to study RNA-protein interactions was described in th...

journal_title：Molecular biotechnology

authors： Yang Y,Wang Q,Guo D

更新日期：2008-09-01 00:00:00

abstract：:Translation initiation of Coxsackievirus B3 (CVB3) RNA is directed by an internal ribosome entry site (IRES) within the 5' untranslated region. Host cell factors involved in this process include some canonical translation factors and additional RNA-binding proteins. We have, previously, described that the Sabin3-like ...

journal_title：Molecular biotechnology

authors： Souii A,M'hadheb-Gharbi MB,Aouni M,Gharbi J

更新日期：2013-06-01 00:00:00

abstract：:The sorbents with immobilized histidine as a pseudo affinity ligand with a wide specificity is described. The possibilities and relevant chemistries to use both particulate and flat or hollow fiber membranes as support matrices are discussed. The usefulness of such adsorbents for the purification of a wide variety of ...

journal_title：Molecular biotechnology

authors： Vijayalakshmi MA

更新日期：1996-12-01 00:00:00

abstract：:Megaprimer-based methodology has been widely applied in site-directed mutagenesis, but rarely used in gene splicing. In this article, we describe a modification of the megaprimer PCR method, which can efficiently create and amplify a specific ligated chimeric gene segment in a PCR reaction and under a common PCR progr...

journal_title：Molecular biotechnology

authors： Chen JR,Lü JJ,Wang HF

更新日期：2008-11-01 00:00:00

abstract：:Heat shock proteins (HSPs) are upregulated in response to stress and play a protective function in refolding of cellular proteins. In hypertension, the heart is a vital organ that requires examination and investigation, and primary induction of HSPs is predominantly effected. Hypertension results from osmotic imbalanc...

journal_title：Molecular biotechnology

authors： Mala JG,Takeuchi S

更新日期：2009-06-01 00:00:00

abstract：:Expression of certain transgenes from an adenovirus vector can be deleterious to its own replication. This can result in the inhibition of virus rescue, reduced viral yields, or, in the worst case, make it impossible to construct a vector expressing the inhibiting transgene product. A gene regulation system based on t...

journal_title：Molecular biotechnology

authors： Gall JG,Lizonova A,EttyReddy D,McVey D,Zuber M,Kovesdi I,Aughtman B,King CR,Brough DE

更新日期：2007-03-01 00:00:00

abstract：:Coptotermes curvignathus is a termite that, owing to its ability to digest living trees, serves as a gold mine for robust industrial enzymes. This unique characteristic reflects the presence of very efficient hydrolytic enzyme systems including cellulases. Transcriptomic analyses of the gut of C. curvignathus revealed...

journal_title：Molecular biotechnology

authors： Woon JS,King PJH,Mackeen MM,Mahadi NM,Wan Seman WMK,Broughton WJ,Abdul Murad AM,Abu Bakar FD

更新日期：2017-07-01 00:00:00

abstract：:IMGT, the International ImMunoGeneTics information system ( http://imgt.cines.fr ), was created in 1989 by the Laboratoire d'ImmunoGénétique Moléculaire (LIGM) (Université Montpellier 2 and CNRS) at Montpellier, France, in order to standardize and manage the complexity of immunogenetics data. IMGT is recognized as the...

journal_title：Molecular biotechnology

authors： Lefranc MP

更新日期：2008-09-01 00:00:00

abstract：:The concept of photoprotection by dietary means is gaining momentum. Plant constituents such as carotenoids and flavonoids are involved in protection against excess light in plants and contribute to the prevention of UV damage in humans. As micronutrients, they are ingested with the diet and are distributed into light...

journal_title：Molecular biotechnology

authors： Stahl W,Sies H

更新日期：2007-09-01 00:00:00

abstract：:D-Glucosamine is a commonly used dietary supplement that promotes cartilage health in humans. Metabolic flux analysis showed that D-glucosamine production could be increased by blocking three pathways involved in the consumption of glucosamine-6-phosphate and acetylglucosamine-6-phosphate. By homologous single-exchang...

journal_title：Molecular biotechnology

authors： Li P,Li X,Wen Z,Dong R,Li K,Fan H,Wang R,Wang J,Lv M

更新日期：2020-08-01 00:00:00

abstract：:Optimization of gene coding-sequence, including preferred codon usage and removal of cryptic splice sites and mRNA-destabilizing motifs, has been shown to improve recombinant protein production of different proteins. Here, we present data to show that gene optimization can also be used to improve the production of a c...

journal_title：Molecular biotechnology

authors： Kalwy S,Rance J,Young R

更新日期：2006-10-01 00:00:00