Rheological properties of cryptococcal polysaccharide change with fiber size, antibody binding and temperature.

Abstract:

:Aim:Cryptococcus neoformans is the major agent of cryptococcosis. The main virulence factor is the polysaccharide (PS) capsule. Changes in cryptococcal PS properties have been poorly elucidated. Materials & methods: We analyzed the mechanical properties of secreted PS and intact capsules, using dynamic light scattering and optical tweezers. Results: Storage and loss moduli showed that secreted PS behaves as a viscoelastic liquid, while capsular PS behaves as a viscoelastic solid. The secreted PS remains as a viscoelastic fluid at different temperatures with thermal hysteresis after 85°C. Antibody binding altered the viscoelastic behavior of both secreted and capsular PS. Conclusion: Deciphering the mechanical aspects of these structures could reveal features that may have consequences in novel therapies against cryptococcosis.

journal_name

Future Microbiol

journal_title

Future microbiology

authors

de S Araújo GR,Viana NB,Pontes B,Frases S

doi

10.2217/fmb-2018-0320

subject

Has Abstract

pub_date

2019-07-01 00:00:00

pages

867-884

eissn

1746-0913

issn

1746-0921

journal_volume

14

pub_type

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