Comparative evaluation of GeneXpert MTB/RIF and multiplex PCR targeting mpb64 and IS6110 for the diagnosis of pleural TB.

Abstract:

AIM:Diagnosis of pleural TB poses serious challenges due to paucibacillary nature of specimens and there is an urgent need to devise a reliable diagnostic test. METHODS:We compared GeneXpert Mycobacterium tuberculosis/rifampin assay and the multiplex PCR (M-PCR) targeting mpb64 (Rv1980c) and IS6110 in pleural fluids (n = 78) of pleural TB patients and non-TB controls. RESULTS:The sensitivities of 89.6 and 33.3%, and specificities of 96.7 and 100%, were observed with M-PCR and Xpert assay, respectively. CONCLUSION:M-PCR showed superiority over Xpert assay and may facilitate an efficient diagnosis of pleural TB.

journal_name

Future Microbiol

journal_title

Future microbiology

authors

Sharma S,Dahiya B,Sreenivas V,Singh N,Raj A,Sheoran A,Yadav A,Gupta KB,Mehta PK

doi

10.2217/fmb-2017-0147

subject

Has Abstract

pub_date

2018-03-01 00:00:00

pages

407-413

eissn

1746-0913

issn

1746-0921

journal_volume

13

pub_type

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