Effects of hyperbaric oxygen on Pseudomonas aeruginosa susceptibility to imipenem and macrophages.

Abstract:

BACKGROUND:The seriousness to treat burn wounds infected with Pseudomonas aeruginosa led us to examine whether the effect of the carbapenem antibiotic imipenem is enhanced by hyperbaric oxygen (HBO). MATERIALS & METHODS:The effects of HBO (100% O2, 3 ATA, 5 h) in combination with imipenen on bacterial counts of six isolates of P. aeruginosa and bacterial ultrastructure were investigated. Infected macrophages were exposed to HBO (100% O2, 3 ATA, 90 min) and the production of reactive oxygen species monitored. RESULTS:HBO enhanced the effects of imipenen. HBO increased superoxide anion production by macrophages and likely kills bacteria by oxidative mechanisms. CONCLUSION:HBO in combination with imipenem can be used to kill P. aeruginosa in vitro and such treatment may be beneficial for the patients with injuries containing the P. aeruginosa.

journal_name

Future Microbiol

journal_title

Future microbiology

authors

Lima FL,Joazeiro PP,Lancellotti M,de Hollanda LM,de Araújo Lima B,Linares E,Augusto O,Brocchi M,Giorgio S

doi

10.2217/fmb.14.111

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

179-89

issue

2

eissn

1746-0913

issn

1746-0921

journal_volume

10

pub_type

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