Abstract:
AIM:To quantitatively assess Staphylococcus aureus biofilms. MATERIALS & METHODS:In addition to the qualitative Congo red agar (CRA) method, we used the bioluminescence (BLM), safranine (SAF), crystal violet (CRV) and resazurin (RES) high-throughput microtiter plate-based quantitative assays. RESULTS:60.47% (26/43) of S. aureus clinical isolates were weak biofilm producers. The CRA method detected positive-slime phenotypes (13.95%), but was unable to distinguish weak from negative producers. BLM assays demonstrated significant correlations with RES (highest), CRV and SAF (lowest). Lower coefficient of variation values indicate precision. BLM scored highest precision (coefficient of variation = 0.013) followed by RES, SAF and CRV. CONCLUSION:BLM and RES detect live biomass in S. aureus biofilms (for physiological studies). SAF and CRV detect live/dead bacteria plus biofilm matrix (for monitoring overall biofilm architecture, not only its cell viability). Reliable assays are essential for effective biofilm therapy.
journal_name
Future Microbioljournal_title
Future microbiologyauthors
Elkhatib WF,Khairalla AS,Ashour HMdoi
10.2217/fmb.14.33subject
Has Abstractpub_date
2014-01-01 00:00:00pages
725-35issue
6eissn
1746-0913issn
1746-0921journal_volume
9pub_type
杂志文章abstract::Over the last decade Raman spectroscopy has become established as a physicochemical technique for the rapid identification of microbes. This powerful analytical method generates a spectroscopic fingerprint from the microbial sample, which provides quantitative and qualitative information that can be used to characteri...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb.11.89
更新日期:2011-09-01 00:00:00
abstract::Chronic hepatitis B virus infection is a global health concern as it affects over 240 million people worldwide and an estimated 686,000 people die annually as a result of complications of the disease. With the development of newer antiviral drugs, viral suppression of HBV is achievable, however elimination of HBV from...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb-2016-0128
更新日期:2016-12-01 00:00:00
abstract::In the past few months, there has been public discussion relating to a new perspective on blood safety and specifically upon measures to prevent or discourage donation by individuals with a diagnosis of myalgic encephalopathy-chronic fatigue syndrome. This reflects an intriguing interplay between science, public healt...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb.11.24
更新日期:2011-04-01 00:00:00
abstract::Evaluation of: Kang S-M, Yoo D-G, Lipatov AS et al.: Induction of long-term protective immune responses by influenza H5N1 virus-like particles. PLoS ONE 4, e4667 (2009). This article describes a long-term protective effect of a vaccine candidate against avian influenza H5N1 that is based on A/Viet Nam1203/04 proteins ...
journal_title:Future microbiology
pub_type: 评论,杂志文章
doi:10.2217/fmb.09.33
更新日期:2009-06-01 00:00:00
abstract:AIMS:Nosocomial infections cause significant morbidity and mortality in cancer patients. As a result of their debilitated immune system, cancer patients are likely candidates for colonization with anaerobes. We sought to compare the distribution of nosocomial infections in neutropenic and non-neutropenic cancer patient...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb.12.125
更新日期:2012-12-01 00:00:00
abstract::Aim: To identify intestinal microbiota compositions in elderly functional constipation (FC) patients. Materials & methods: Fecal samples from 61 FC patients and 48 healthy age-matched volunteers were analyzed through 16S rRNA gene sequencing. Results: The intestinal microbiota compositions of FC patients were signific...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2019-0283
更新日期:2020-02-01 00:00:00
abstract::Salmonella enterica serovars Typhi and Paratyphi are the causative agents of human typhoid fever. Current typhoid vaccines are ineffective and are not widely used in endemic areas. Greater understanding of host-pathogen interactions during Salmonella infection should facilitate the development of improved vaccines to ...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.14.98
更新日期:2015-01-01 00:00:00
abstract::Using the murine CMV animal model and the well-established model of Cre-lox-P-mediated green-fluorescence tagging of endothelial cell (EC)-derived mouse CMV to quantify the role of infected ECs in transplantation-associated CMV dissemination (in mice expressing Cre recombinase under the control of either the Tie2 or t...
journal_title:Future microbiology
pub_type: 评论,杂志文章
doi:10.2217/fmb.12.12
更新日期:2012-04-01 00:00:00
abstract::Evaluation of: Mowat E, Butcher J, Lang S, Williams C, Ramage G: Development of a simple model for studying the effects of antifungal agents on multicellular communities of Aspergillus fumigatus. J. Med. Microbiol. 56, 1205-1212 (2007). Many microorganisms possess the innate ability for adhering to biotic and abiotic ...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/17460913.3.1.19
更新日期:2008-02-01 00:00:00
abstract::Influenza B viruses (IBVs) circulate annually along with influenza A (IAV) strains during seasonal epidemics. IBV can dominate influenza seasons and cause severe disease, particularly in children and adolescents. Research has revealed interesting aspects of IBV and highlighted the importance of these viruses in clinic...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.15.120
更新日期:2016-01-01 00:00:00
abstract:AIMS:This study aimed to evaluate the antimicrobial activity of two different formulations of bioglass BAG-S53P4 against multiresistant microorganisms involved in bone infections, and the capability of bioglass to select for resistance. METHODS:Antibacterial activity was evaluated by means of killing curves. The abili...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/FMB.15.57
更新日期:2015-01-01 00:00:00
abstract::Bacillus anthracis is a Gram-positive, spore-forming bacterium representing the etiological cause of anthrax, a rare lethal disease of animals and humans. Development of anthrax countermeasures has gained increasing attention owing to the potential use of B. anthracis spores as a bioterror weapon. The various forms of...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.09.73
更新日期:2009-10-01 00:00:00
abstract:AIM:To investigate the role of Cat1 overproduction in Candida albicans. MATERIALS & METHODS:Strains overproducing the CAT1 gene were constructed. RESULTS:Cells overproducing CAT1 were found to be more resistant to some oxidants and mammalian phagocytic cells. They also showed reduced intracellular reactive oxygen spe...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2016-0067
更新日期:2016-10-01 00:00:00
abstract::Background: Sporotrichosis occurs through contact with contaminated soil and plant. However, the incidence of sporotrichosis as a zoonotic epidemic has increased, particularly in Rio de Janeiro. Aim: In this work, we decided to evaluate some T-cell phenotypes involved in the immune response. Materials & methods: We us...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2020-0046
更新日期:2020-09-01 00:00:00
abstract::Amoebiasis is a serious infectious disease that is caused by the unicellular parasite, Entamoeba histolytica. This parasite is mainly found in developing countries, and are named owing to its ability to destroy tissues. The molecular mechanisms that regulate the virulence of this parasite are not well understood. In r...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.10.140
更新日期:2010-12-01 00:00:00
abstract::Report from the 28th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID 2018), Madrid, Spain, 21-24 April 2018 Gram-negative bacteria such as Klebsiella, Acinetobacter and Pseudomonas cause some of the most serious infections and are increasingly resistant to multiple drugs and in some cases, t...
journal_title:Future microbiology
pub_type:
doi:10.2217/fmb-2018-0202
更新日期:2018-10-01 00:00:00
abstract:AIM:To evaluate and characterize the etiopathogenesis of the fusarial onychomycosis in an ex vivo study through fragments of sterile human nail, without the addition of any nutritional source. MATERIALS & METHODS:The infection and invasion of Fusarium oxysporum in the nail were evaluated by scanning electron microscop...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2018-0245
更新日期:2018-12-01 00:00:00
abstract::Aim:Helicobacter pylori is usually detected based on hematoxylin-eosin (H-E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performed and evaluated H-E, IHC...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2019-0280
更新日期:2020-08-01 00:00:00
abstract::Capabilities in mass spectrometry are evolving rapidly, with recent improvements in sensitivity, data analysis and, most important from the standpoint of this review, much higher throughput, allowing analysis of many samples in a single day. This short review describes how these improvements in mass spectrometry can b...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/17460913.3.6.625
更新日期:2008-12-01 00:00:00
abstract::Recognition of bacterial infection is the first key step to the initiation of an inflammatory response and host defense. Transmembrane proteins of the Toll-like receptor family have long been recognized as key detectors of the extracellular presence of pathogens. Recently, much research has identified a variety of int...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/17460913.4.1.65
更新日期:2009-02-01 00:00:00
abstract::Atomic force microscopy (AFM) has recently opened a variety of novel possibilities for imaging and manipulating microbial surfaces in their native environment. While AFM imaging offers a means to visualize surface structures at high resolution and in physiological conditions, AFM force spectroscopy enables researchers...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/17460913.1.4.387
更新日期:2006-12-01 00:00:00
abstract::The history of vaccines for respiratory syncytial virus (RSV) illustrates the complex immunity and immunopathology to this ubiquitous virus, starting from the failed formalin-inactivated vaccine trials performed in the 1960s. An attractive alternative to traditional live or killed virus vaccines is a defined vaccine c...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.10.22
更新日期:2010-04-01 00:00:00
abstract::Anaerobes represent the dominating population in the human gut microbiota and play a key role in gut homeostasis. In addition, several anaerobes are now considered as probiotics and they remain essential to several processes in the field of biotechnology. With the implementation of MALDI-TOF MS in routine laboratories...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb-2017-0170
更新日期:2018-03-01 00:00:00
abstract:AIM:To assess different concentrations and formulations of topical ozenoxacin using a mouse model of Staphylococcus aureus dermal infection for identification of the best formulation for treating patients with impetigo. MATERIALS & METHODS:The efficacy of ozenoxacin formulations was compared with vehicle control, mupi...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2017-0290
更新日期:2018-05-01 00:00:00
abstract::Aim: To investigate the action mechanism of 1-benzyl-1,4-diazepane (1-BD) as efflux pump inhibitor (EPI) in Escherichia coli mutants: ΔacrAB or overexpressing AcrAB and AcrEF efflux pumps. Materials & methods: Effect of 1-BD on: antibiotic potentiation, by microdilution method; membrane functionality, by fluorimetric ...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2019-0296
更新日期:2020-07-01 00:00:00
abstract::Background: There is increasing evidence of the association between microbiome dysfunction and Parkinson's disease (PD). Moreover, some PD patients suffer from unintentional weight loss (WL) which may precede the motor manifestations of the disease. Materials & methods: Gut microbiota profiling by 16S rRNA gene sequen...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2019-0336
更新日期:2020-09-01 00:00:00
abstract:AIMS:To investigate the antitumor effects of probiotics Clostridium butyricum and Bacillus subtilis on colorectal cancer (CRC) progression. MATERIALS & METHODS:The effects of C. butyricum and B. subtilis on CRC cells were studied. Male C57BL/6 mice with 1,2-dimethylhydrazine dihydrochloride (DMH)-induced CRC were inte...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb.15.66
更新日期:2015-01-01 00:00:00
abstract:AIM:We evaluated a Staphylococcus argenteus-specific diagnostic profile of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer for accurate identification of this novel bacterium. MATERIALS & METHODS:Staphylococcus argenteus was identified based on negative crtM gene and presence of specific s...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2018-0015
更新日期:2018-07-01 00:00:00
abstract::Currently, there is growing interest in the identification and purification of microbial lectins due to their involvement in the pathogenicity mechanisms of pathogens, such as Entamoeba histolytica and free-living amoebae. The Gal/GalNAc lectin from E. histolytica participates in adhesion, cytotoxicity and regulation ...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2019-0275
更新日期:2020-07-01 00:00:00
abstract::Evaluation of: Belogurov GA, Vassylyeva MN, Sevostyanova A et al.: Transcription inactivation through local refolding of the RNA polymerase structure. Nature 457, 332-335 (2008) and, Mukhopadhyay J, Das K, Ismail S et al.: The RNA polymerase 'switch region' is a target for inhibitors. Cell 135, 295-307 (2008). Bacteri...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/17460913.4.2.145
更新日期:2009-03-01 00:00:00