Evaluation of different microtiter plate-based methods for the quantitative assessment of Staphylococcus aureus biofilms.

Abstract:

AIM:To quantitatively assess Staphylococcus aureus biofilms. MATERIALS & METHODS:In addition to the qualitative Congo red agar (CRA) method, we used the bioluminescence (BLM), safranine (SAF), crystal violet (CRV) and resazurin (RES) high-throughput microtiter plate-based quantitative assays. RESULTS:60.47% (26/43) of S. aureus clinical isolates were weak biofilm producers. The CRA method detected positive-slime phenotypes (13.95%), but was unable to distinguish weak from negative producers. BLM assays demonstrated significant correlations with RES (highest), CRV and SAF (lowest). Lower coefficient of variation values indicate precision. BLM scored highest precision (coefficient of variation = 0.013) followed by RES, SAF and CRV. CONCLUSION:BLM and RES detect live biomass in S. aureus biofilms (for physiological studies). SAF and CRV detect live/dead bacteria plus biofilm matrix (for monitoring overall biofilm architecture, not only its cell viability). Reliable assays are essential for effective biofilm therapy.

journal_name

Future Microbiol

journal_title

Future microbiology

authors

Elkhatib WF,Khairalla AS,Ashour HM

doi

10.2217/fmb.14.33

subject

Has Abstract

pub_date

2014-01-01 00:00:00

pages

725-35

issue

6

eissn

1746-0913

issn

1746-0921

journal_volume

9

pub_type

杂志文章
  • Raman spectroscopy: lighting up the future of microbial identification.

    abstract::Over the last decade Raman spectroscopy has become established as a physicochemical technique for the rapid identification of microbes. This powerful analytical method generates a spectroscopic fingerprint from the microbial sample, which provides quantitative and qualitative information that can be used to characteri...

    journal_title:Future microbiology

    pub_type: 杂志文章

    doi:10.2217/fmb.11.89

    authors: Ashton L,Lau K,Winder CL,Goodacre R

    更新日期:2011-09-01 00:00:00

  • Treatment of hepatitis B virus: an update.

    abstract::Chronic hepatitis B virus infection is a global health concern as it affects over 240 million people worldwide and an estimated 686,000 people die annually as a result of complications of the disease. With the development of newer antiviral drugs, viral suppression of HBV is achievable, however elimination of HBV from...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审

    doi:10.2217/fmb-2016-0128

    authors: Ward H,Tang L,Poonia B,Kottilil S

    更新日期:2016-12-01 00:00:00

  • Chronic fatigue syndrome, XMRV and blood safety.

    abstract::In the past few months, there has been public discussion relating to a new perspective on blood safety and specifically upon measures to prevent or discourage donation by individuals with a diagnosis of myalgic encephalopathy-chronic fatigue syndrome. This reflects an intriguing interplay between science, public healt...

    journal_title:Future microbiology

    pub_type: 杂志文章

    doi:10.2217/fmb.11.24

    authors: Dodd RY

    更新日期:2011-04-01 00:00:00

  • Preclinical efficacy of a virus-like particle-based vaccine against avian influenza H5N1.

    abstract::Evaluation of: Kang S-M, Yoo D-G, Lipatov AS et al.: Induction of long-term protective immune responses by influenza H5N1 virus-like particles. PLoS ONE 4, e4667 (2009). This article describes a long-term protective effect of a vaccine candidate against avian influenza H5N1 that is based on A/Viet Nam1203/04 proteins ...

    journal_title:Future microbiology

    pub_type: 评论,杂志文章

    doi:10.2217/fmb.09.33

    authors: Arnon R,Ben-Yedidia T

    更新日期:2009-06-01 00:00:00

  • Nosocomial infections in leukemic and solid-tumor cancer patients: distribution, outcome and microbial spectrum of anaerobes.

    abstract:AIMS:Nosocomial infections cause significant morbidity and mortality in cancer patients. As a result of their debilitated immune system, cancer patients are likely candidates for colonization with anaerobes. We sought to compare the distribution of nosocomial infections in neutropenic and non-neutropenic cancer patient...

    journal_title:Future microbiology

    pub_type: 杂志文章

    doi:10.2217/fmb.12.125

    authors: El-Sharif A,Elkhatib WF,Ashour HM

    更新日期:2012-12-01 00:00:00

  • The composition of intestinal microbiota and its association with functional constipation of the elderly patients.

    abstract::Aim: To identify intestinal microbiota compositions in elderly functional constipation (FC) patients. Materials & methods: Fecal samples from 61 FC patients and 48 healthy age-matched volunteers were analyzed through 16S rRNA gene sequencing. Results: The intestinal microbiota compositions of FC patients were signific...

    journal_title:Future microbiology

    pub_type: 杂志文章

    doi:10.2217/fmb-2019-0283

    authors: Guo M,Yao J,Yang F,Liu W,Bai H,Ma J,Ma X,Zhang J,Fang Y,Miao Y,Sun J,Zhang Y,Zhao H

    更新日期:2020-02-01 00:00:00

  • Protective host immune responses to Salmonella infection.

    abstract::Salmonella enterica serovars Typhi and Paratyphi are the causative agents of human typhoid fever. Current typhoid vaccines are ineffective and are not widely used in endemic areas. Greater understanding of host-pathogen interactions during Salmonella infection should facilitate the development of improved vaccines to ...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审

    doi:10.2217/fmb.14.98

    authors: Pham OH,McSorley SJ

    更新日期:2015-01-01 00:00:00

  • Endothelial cells and CMV dissemination.

    abstract::Using the murine CMV animal model and the well-established model of Cre-lox-P-mediated green-fluorescence tagging of endothelial cell (EC)-derived mouse CMV to quantify the role of infected ECs in transplantation-associated CMV dissemination (in mice expressing Cre recombinase under the control of either the Tie2 or t...

    journal_title:Future microbiology

    pub_type: 评论,杂志文章

    doi:10.2217/fmb.12.12

    authors: Gerna G

    更新日期:2012-04-01 00:00:00

  • Do Aspergillus species produce biofilm?

    abstract::Evaluation of: Mowat E, Butcher J, Lang S, Williams C, Ramage G: Development of a simple model for studying the effects of antifungal agents on multicellular communities of Aspergillus fumigatus. J. Med. Microbiol. 56, 1205-1212 (2007). Many microorganisms possess the innate ability for adhering to biotic and abiotic ...

    journal_title:Future microbiology

    pub_type: 杂志文章

    doi:10.2217/17460913.3.1.19

    authors: Chandrasekar PH,Manavathu EK

    更新日期:2008-02-01 00:00:00

  • Knowns and unknowns of influenza B viruses.

    abstract::Influenza B viruses (IBVs) circulate annually along with influenza A (IAV) strains during seasonal epidemics. IBV can dominate influenza seasons and cause severe disease, particularly in children and adolescents. Research has revealed interesting aspects of IBV and highlighted the importance of these viruses in clinic...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审

    doi:10.2217/fmb.15.120

    authors: Koutsakos M,Nguyen TH,Barclay WS,Kedzierska K

    更新日期:2016-01-01 00:00:00

  • Antimicrobial activity and resistance selection of different bioglass S53P4 formulations against multidrug resistant strains.

    abstract:AIMS:This study aimed to evaluate the antimicrobial activity of two different formulations of bioglass BAG-S53P4 against multiresistant microorganisms involved in bone infections, and the capability of bioglass to select for resistance. METHODS:Antibacterial activity was evaluated by means of killing curves. The abili...

    journal_title:Future microbiology

    pub_type: 杂志文章

    doi:10.2217/FMB.15.57

    authors: Drago L,De Vecchi E,Bortolin M,Toscano M,Mattina R,Romanò CL

    更新日期:2015-01-01 00:00:00

  • Proteomic studies of Bacillus anthracis.

    abstract::Bacillus anthracis is a Gram-positive, spore-forming bacterium representing the etiological cause of anthrax, a rare lethal disease of animals and humans. Development of anthrax countermeasures has gained increasing attention owing to the potential use of B. anthracis spores as a bioterror weapon. The various forms of...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审

    doi:10.2217/fmb.09.73

    authors: Chitlaru T,Shafferman A

    更新日期:2009-10-01 00:00:00

  • Role of catalase overproduction in drug resistance and virulence in Candida albicans.

    abstract:AIM:To investigate the role of Cat1 overproduction in Candida albicans. MATERIALS & METHODS:Strains overproducing the CAT1 gene were constructed. RESULTS:Cells overproducing CAT1 were found to be more resistant to some oxidants and mammalian phagocytic cells. They also showed reduced intracellular reactive oxygen spe...

    journal_title:Future microbiology

    pub_type: 杂志文章

    doi:10.2217/fmb-2016-0067

    authors: Román E,Prieto D,Martin R,Correia I,Mesa Arango AC,Alonso-Monge R,Zaragoza O,Pla J

    更新日期:2016-10-01 00:00:00

  • Tregs in the immune response of BALB/c mice experimentally infected with species of the Sporothrix genus.

    abstract::Background: Sporotrichosis occurs through contact with contaminated soil and plant. However, the incidence of sporotrichosis as a zoonotic epidemic has increased, particularly in Rio de Janeiro. Aim: In this work, we decided to evaluate some T-cell phenotypes involved in the immune response. Materials & methods: We us...

    journal_title:Future microbiology

    pub_type: 杂志文章

    doi:10.2217/fmb-2020-0046

    authors: Corrêa-Moreira D,De Luca PM,Romeo O,C Menezes R,Paes RA,Oliveira RZ,de Moraes AM,de L Neto RG,Moraes Borba C,E de Oliveira MM

    更新日期:2020-09-01 00:00:00

  • Epigenetics in the unicellular parasite Entamoeba histolytica.

    abstract::Amoebiasis is a serious infectious disease that is caused by the unicellular parasite, Entamoeba histolytica. This parasite is mainly found in developing countries, and are named owing to its ability to destroy tissues. The molecular mechanisms that regulate the virulence of this parasite are not well understood. In r...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审

    doi:10.2217/fmb.10.140

    authors: Tovy A,Ankri S

    更新日期:2010-12-01 00:00:00

  • Know your enemy: managing resistant Gram-negative infections.

    abstract::Report from the 28th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID 2018), Madrid, Spain, 21-24 April 2018 Gram-negative bacteria such as Klebsiella, Acinetobacter and Pseudomonas cause some of the most serious infections and are increasingly resistant to multiple drugs and in some cases, t...

    journal_title:Future microbiology

    pub_type:

    doi:10.2217/fmb-2018-0202

    authors: Eckmann C,Rojas LJ,Lyon S

    更新日期:2018-10-01 00:00:00

  • Fusarium oxysporum is an onychomycosis etiopathogenic agent.

    abstract:AIM:To evaluate and characterize the etiopathogenesis of the fusarial onychomycosis in an ex vivo study through fragments of sterile human nail, without the addition of any nutritional source. MATERIALS & METHODS:The infection and invasion of Fusarium oxysporum in the nail were evaluated by scanning electron microscop...

    journal_title:Future microbiology

    pub_type: 杂志文章

    doi:10.2217/fmb-2018-0245

    authors: Veiga FF,de Castro-Hoshino LV,Sato F,Bombassaro A,Vicente VA,Mendes V,Baesso ML,Negri M,Svidzinski TI

    更新日期:2018-12-01 00:00:00

  • Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR.

    abstract::Aim:Helicobacter pylori is usually detected based on hematoxylin-eosin (H-E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performed and evaluated H-E, IHC...

    journal_title:Future microbiology

    pub_type: 杂志文章

    doi:10.2217/fmb-2019-0280

    authors: A Castaneda C,Castillo M,Sanchez J,Casavilca S,Sanchez J,A Bernabe L,Suarez N,Chavez I,Ruiz E,Tello K,R Villa M,Zevallos R,Montenegro P,Dias-Neto E,Landa-Baella M,Taxa L

    更新日期:2020-08-01 00:00:00

  • Use of high-throughput mass spectrometry to elucidate host-pathogen interactions in Salmonella.

    abstract::Capabilities in mass spectrometry are evolving rapidly, with recent improvements in sensitivity, data analysis and, most important from the standpoint of this review, much higher throughput, allowing analysis of many samples in a single day. This short review describes how these improvements in mass spectrometry can b...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审

    doi:10.2217/17460913.3.6.625

    authors: Rodland KD,Adkins JN,Ansong C,Chowdhury S,Manes NP,Shi L,Yoon H,Smith RD,Heffron F

    更新日期:2008-12-01 00:00:00

  • Bacterial triggering of inflammation by intracellular sensors.

    abstract::Recognition of bacterial infection is the first key step to the initiation of an inflammatory response and host defense. Transmembrane proteins of the Toll-like receptor family have long been recognized as key detectors of the extracellular presence of pathogens. Recently, much research has identified a variety of int...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审

    doi:10.2217/17460913.4.1.65

    authors: Evans TJ

    更新日期:2009-02-01 00:00:00

  • Nanoscale exploration of microbial surfaces using the atomic force microscope.

    abstract::Atomic force microscopy (AFM) has recently opened a variety of novel possibilities for imaging and manipulating microbial surfaces in their native environment. While AFM imaging offers a means to visualize surface structures at high resolution and in physiological conditions, AFM force spectroscopy enables researchers...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审

    doi:10.2217/17460913.1.4.387

    authors: Dufrêne YF

    更新日期:2006-12-01 00:00:00

  • Prospects for defined epitope vaccines for respiratory syncytial virus.

    abstract::The history of vaccines for respiratory syncytial virus (RSV) illustrates the complex immunity and immunopathology to this ubiquitous virus, starting from the failed formalin-inactivated vaccine trials performed in the 1960s. An attractive alternative to traditional live or killed virus vaccines is a defined vaccine c...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审

    doi:10.2217/fmb.10.22

    authors: Anderson R,Huang Y,Langley JM

    更新日期:2010-04-01 00:00:00

  • Methods for culturing anaerobes from human specimen.

    abstract::Anaerobes represent the dominating population in the human gut microbiota and play a key role in gut homeostasis. In addition, several anaerobes are now considered as probiotics and they remain essential to several processes in the field of biotechnology. With the implementation of MALDI-TOF MS in routine laboratories...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审

    doi:10.2217/fmb-2017-0170

    authors: Guilhot E,Khelaifia S,La Scola B,Raoult D,Dubourg G

    更新日期:2018-03-01 00:00:00

  • Therapeutic efficacy of ozenoxacin in animal models of dermal infection with Staphylococcus aureus.

    abstract:AIM:To assess different concentrations and formulations of topical ozenoxacin using a mouse model of Staphylococcus aureus dermal infection for identification of the best formulation for treating patients with impetigo. MATERIALS & METHODS:The efficacy of ozenoxacin formulations was compared with vehicle control, mupi...

    journal_title:Future microbiology

    pub_type: 杂志文章

    doi:10.2217/fmb-2017-0290

    authors: Tarragó C,Esquirol LP,Arañó A,Lachamp L,D'Aniello F,Zsolt I

    更新日期:2018-05-01 00:00:00

  • 1-benzyl-1,4-diazepane reduces the efflux of resistance-nodulation-cell division pumps in Escherichia coli.

    abstract::Aim: To investigate the action mechanism of 1-benzyl-1,4-diazepane (1-BD) as efflux pump inhibitor (EPI) in Escherichia coli mutants: ΔacrAB or overexpressing AcrAB and AcrEF efflux pumps. Materials & methods: Effect of 1-BD on: antibiotic potentiation, by microdilution method; membrane functionality, by fluorimetric ...

    journal_title:Future microbiology

    pub_type: 杂志文章

    doi:10.2217/fmb-2019-0296

    authors: Casalone E,Vignolini T,Braconi L,Gardini L,Capitanio M,Pavone FS,Dei S,Teodori E

    更新日期:2020-07-01 00:00:00

  • The impact of intestinal microbiota on weight loss in Parkinson's disease patients: a pilot study.

    abstract::Background: There is increasing evidence of the association between microbiome dysfunction and Parkinson's disease (PD). Moreover, some PD patients suffer from unintentional weight loss (WL) which may precede the motor manifestations of the disease. Materials & methods: Gut microbiota profiling by 16S rRNA gene sequen...

    journal_title:Future microbiology

    pub_type: 杂志文章

    doi:10.2217/fmb-2019-0336

    authors: Del Chierico F,Grassini P,Quagliariello A,Torti M,Russo A,Reddel S,Stocchi F

    更新日期:2020-09-01 00:00:00

  • Probiotics Clostridium butyricum and Bacillus subtilis ameliorate intestinal tumorigenesis.

    abstract:AIMS:To investigate the antitumor effects of probiotics Clostridium butyricum and Bacillus subtilis on colorectal cancer (CRC) progression. MATERIALS & METHODS:The effects of C. butyricum and B. subtilis on CRC cells were studied. Male C57BL/6 mice with 1,2-dimethylhydrazine dihydrochloride (DMH)-induced CRC were inte...

    journal_title:Future microbiology

    pub_type: 杂志文章

    doi:10.2217/fmb.15.66

    authors: Chen ZF,Ai LY,Wang JL,Ren LL,Yu YN,Xu J,Chen HY,Yu J,Li M,Qin WX,Ma X,Shen N,Chen YX,Hong J,Fang JY

    更新日期:2015-01-01 00:00:00

  • Accurate differentiation of novel Staphylococcus argenteus from Staphylococcus aureus using MALDI-TOF MS.

    abstract:AIM:We evaluated a Staphylococcus argenteus-specific diagnostic profile of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer for accurate identification of this novel bacterium. MATERIALS & METHODS:Staphylococcus argenteus was identified based on negative crtM gene and presence of specific s...

    journal_title:Future microbiology

    pub_type: 杂志文章

    doi:10.2217/fmb-2018-0015

    authors: Chen SY,Lee H,Teng SH,Wang XM,Lee TF,Huang YC,Liao CH,Teng LJ,Hsueh PR

    更新日期:2018-07-01 00:00:00

  • Lectins as virulence factors in Entamoeba histolytica and free-living amoebae.

    abstract::Currently, there is growing interest in the identification and purification of microbial lectins due to their involvement in the pathogenicity mechanisms of pathogens, such as Entamoeba histolytica and free-living amoebae. The Gal/GalNAc lectin from E. histolytica participates in adhesion, cytotoxicity and regulation ...

    journal_title:Future microbiology

    pub_type: 杂志文章

    doi:10.2217/fmb-2019-0275

    authors: Guzmán-Téllez P,Martínez-Castillo M,Flores-Huerta N,Rosales-Morgan G,Pacheco-Yépez J,la Garza M,Serrano-Luna J,Shibayama M

    更新日期:2020-07-01 00:00:00

  • Myxopyronin: a punch in the jaws of bacterial RNA polymerase.

    abstract::Evaluation of: Belogurov GA, Vassylyeva MN, Sevostyanova A et al.: Transcription inactivation through local refolding of the RNA polymerase structure. Nature 457, 332-335 (2008) and, Mukhopadhyay J, Das K, Ismail S et al.: The RNA polymerase 'switch region' is a target for inhibitors. Cell 135, 295-307 (2008). Bacteri...

    journal_title:Future microbiology

    pub_type: 杂志文章

    doi:10.2217/17460913.4.2.145

    authors: Tupin A,Gualtieri M,Brodolin K,Leonetti JP

    更新日期:2009-03-01 00:00:00