Abstract:
:The endo-levanase BT1760 of a human gut commensal Bacteroides thetaiotaomicron randomly cuts a β-2,6-linked fructan, levan, into fructo-oligosaccharides providing a prebiotic substrate for gut microbiota. Here we introduce the crystal structure of BT1760 at resolution of 1.65 Å. The fold of the enzyme is typical for GH32 family proteins: a catalytic N-terminal five-bladed β-propeller connected with a C-terminal β-sandwich domain. The levantetraose-bound structure of catalytically inactive mutant E221A at 1.90-Å resolution reveals differences in substrate binding between the endo-acting fructanases. A shallow substrate-binding pocket of the endo-inulinase INU2 of Aspergillus ficuum binds at least three fructose residues at its flat bottom. In the levantetraose-soaked crystal of the endo-levanase E221A mutant the ligand was bent into the pond-like substrate pocket with its fructose residues making contacts at -3, -2, -1 and + 1 subsites residing at several pocket depths. Binding of levantetraose to the β-sandwich domain was not detected. The N- and C-terminal modules of BT1760 did not bind levan if expressed separately, the catalytic domain lost its activity and both modules tended to precipitate. We gather that endo-levanase BT1760 requires both domains for correct folding, solubility and stability of the protein.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Ernits K,Eek P,Lukk T,Visnapuu T,Alamäe Tdoi
10.1038/s41598-019-44785-0subject
Has Abstractpub_date
2019-06-11 00:00:00pages
8443issue
1issn
2045-2322pii
10.1038/s41598-019-44785-0journal_volume
9pub_type
杂志文章abstract::The exact extent, by which the hydrologic cycle in the Neotropics was affected by external forcing during the last deglaciation, remains poorly understood. Here we present a new paleo-rainfall reconstruction based on high-resolution speleothem δ18O records from the core region of the South American Monsoon System (SAM...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44267
更新日期:2017-03-10 00:00:00
abstract::High-throughput technologies have identified significant changes in patterns of mRNA expression over cancer development but the functional significance of these changes often rests upon the assumption that observed changes in levels of mRNA accurately reflect changes in levels of their encoded proteins. We systematica...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08502-z
更新日期:2017-08-15 00:00:00
abstract::Colorectal cancer is one of the main causes of cancer death worldwide, and novel biomarkers are urgently needed for its early diagnosis and treatment. The utilization of metabolomics to identify and quantify metabolites in body fluids may allow the detection of changes in their concentrations that could serve as diagn...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55952-8
更新日期:2019-12-27 00:00:00
abstract::The association between perioperative blood transfusion (PBT) with adverse oncological outcomes have been previously reported in multiple malignancies including RCC. Nevertheless, the importance of transfusion timing is still unclear. The primary purpose of this study is to appraise whether the receipt of intraoperati...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37691-4
更新日期:2019-02-04 00:00:00
abstract::The current study aimed to evaluate the efficacy of linked color imaging (LCI) in improving the visibility of superficial non-ampullary duodenal epithelial tumors (SNADETs). We prospectively evaluated 44 consecutive patients diagnosed with SNADETs. Three trainees and three experts assessed the visibility scores of whi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77726-3
更新日期:2020-11-26 00:00:00
abstract::The crystallization characteristics in supercooled liquid Zn during isothermal relaxation were investigated using molecular dynamics simulations by adopting the cluster-type index method (CTIM) and the tracing method. Results showed that the crystallization process undergo three different stages. The size of the criti...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep31653
更新日期:2016-08-16 00:00:00
abstract::Externally manipulating memories by presenting conditioned stimuli (CS) during sleep is a new approach to investigating memory processing during sleep. However, whether presenting a CS during REM or NREM sleep enhances or extinguishes fear memory has not been clearly delineated. In this study, mice underwent trace fea...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46247
更新日期:2017-04-12 00:00:00
abstract::Increasing evidence suggest that the glucose-lowering drug metformin exerts a valuable anti-senescence role. The ability of metformin to affect the biogenesis of selected microRNAs (miRNAs) was recently suggested. MicroRNA isoforms (isomiRs) are distinct variations of miRNA sequences, harboring addition or deletion of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-78871-5
更新日期:2020-12-11 00:00:00
abstract::Gram-negative bacteria belonging to the genus Klebsiella are important nosocomial pathogens, readily acquiring resistance to all known antibiotics. Bacteriocins, non-antibiotic antibacterial proteins, have been earlier proposed as potential therapeutic agents for control of other Gram-negative species such as Escheric...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51969-1
更新日期:2019-10-28 00:00:00
abstract::The vast majority of antibody-drug conjugates (ADC) are prepared through amine-to-thiol conjugation. To date, N-Succinimidyl-4-(maleimidomethyl) cyclohexanecarboxylate (SMCC) has been one of the most frequently applied reagents for the preparation of ADC and other functional conjugates. However, SMCC-based conjugates ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30835
更新日期:2016-08-09 00:00:00
abstract::A subset of the family of fluorescent proteins are the non-fluorescent chromoproteins which are promising probe molecules for use in photoacoustic imaging and as acceptor chromophores in Förster resonance energy transfer (FRET)-based biosensors. Typical approaches for fluorescent protein optimization by screening of l...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep22129
更新日期:2016-03-01 00:00:00
abstract::A variety of environmental factors contribute to progressive development of osteoarthritis (OA). Environmental factors that upset circadian rhythms have been linked to various diseases. Our recent work establishes chronic environmental circadian disruption - analogous to rotating shiftwork-associated disruption of cir...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16896
更新日期:2015-11-20 00:00:00
abstract::The ability for magnetite to act as a recyclable electron donor and acceptor for Fe-metabolizing bacteria has recently been shown. However, it remains poorly understood whether microbe-mineral interfacial electron transfer processes are limited by the redox capacity of the magnetite surface or that of whole particles....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30969
更新日期:2016-08-05 00:00:00
abstract::Propionibacterium acnes (P. acnes) is a major skin-associated bacterium that was long considered commensal, until several studies revealed it to be an opportunistic pathogen. We investigated the ability of P. acnes surface proteins to recognize ECM proteins and showed that a 58 kDa P. acnes surface protein was specifi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06940-3
更新日期:2017-07-25 00:00:00
abstract::The development of electrochemical biosensors for the simultaneous detection of ascorbic acid (AA), dopamine (DA), uric acid (UA), tryptophan (Trp), and nitrite ([Formula: see text]) in human serum is reported in this work. Free-standing graphene nanosheets were fabricated on Ta wire using the chemical vapor depositio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07646-2
更新日期:2017-08-01 00:00:00
abstract::The development of the optical bio-chemical sensing technology is an extremely important scientific and technological issue for diagnosis and monitoring of diseases, control of industrial processes, environmental detection of air and water pollutants. Owing to their distinctive features, chalcogenide amorphous thin fi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-03678-w
更新日期:2017-06-14 00:00:00
abstract::Overexpression of hST3Gal1 leads to hypersialylation of cell-surface glycoconjugates, a cancer-associated condition that promotes cell growth, migration and invasion. Upregulation of this enzyme in ovarian cancer is linked to cancer progression and metastasis, contributing also to chemotherapy resistance. Strategies f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-54384-8
更新日期:2019-11-29 00:00:00
abstract::Transcriptional enhancers are non-coding segments of DNA that play a central role in the spatiotemporal regulation of gene expression programs. However, systematically and precisely predicting enhancers remain a major challenge. Although existing methods have achieved some success in enhancer prediction, they still su...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28517
更新日期:2016-06-22 00:00:00
abstract::Though cognitive function is proven to be an independent predictor of survival in patients with intrinsic brain tumors, cognitive functions are still rarely considered. Aim of this study was to assess neurocognitive function and to identify risk factors for neurocognitive deficits. 103 patients with primary neuroepith...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17833-w
更新日期:2017-12-19 00:00:00
abstract::If the components of a battery, including electrodes, separator, electrolyte and the current collectors can be designed as paints and applied sequentially to build a complete battery, on any arbitrary surface, it would have significant impact on the design, implementation and integration of energy storage devices. Her...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00481
更新日期:2012-01-01 00:00:00
abstract::The ATP-binding cassette transporter TAPL translocates polypeptides from the cytosol into the lysosomal lumen. TAPL can be divided into two functional units: coreTAPL, active in ATP-dependent peptide translocation, and the N-terminal membrane spanning domain, TMD0, responsible for cellular localization and interaction...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33841-w
更新日期:2018-10-23 00:00:00
abstract::The hepatic asialoglycoprotein receptor (ASGP-R) is posttranslationally modified in the Golgi en route to the plasma membrane, where it mediates clearance of desialylated serum glycoproteins. It is known that content of plasma membrane-associated ASGP-R is decreased after ethanol exposure, although the mechanisms rema...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17127
更新日期:2015-11-26 00:00:00
abstract::Adenoviruses are very efficient vectors for delivering therapeutic genes in preclinical and clinical trials. However, randomized controlled human trials have often been lacking clear clinically relevant results. We hypothesized that high lipid levels and specific lipoproteins could significantly decrease adenoviral tr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00376-5
更新日期:2017-03-24 00:00:00
abstract::People with normal hearing thresholds can nonetheless have difficulty with understanding speech in noisy backgrounds. The origins of such supra-threshold hearing deficits remain largely unclear. Previously we showed that the auditory brainstem response to running speech is modulated by selective attention, evidencing ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50773-1
更新日期:2019-10-01 00:00:00
abstract::The primary hurdle for diagnosis of some diseases is the long incubation required to culture and confirm the presence of bacteria. The concept of using microbial VOCs as "signature markers" could provide a faster and noninvasive diagnosis. Finding biomarkers is challenging due to the specificity required in complex ma...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47907-w
更新日期:2019-08-20 00:00:00
abstract::Vaccine platforms that can be flexibly loaded with antigens can contribute to decrease response time to emerging infections. For many pathogens and chronic infections, induction of a robust cytotoxic T lymphocytes-mediated response is desirable to control infection. Antigen delivery into the cytoplasm of antigen prese...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08787-0
更新日期:2017-08-29 00:00:00
abstract::Orthographic regularity is important for processing Chinese characters. However, the issues how orthographic regularity influences the visual recognition of real Chinese characters and whether common processes related to the potential effect exist between successive (SUCC) and concurrent (CONC) conditions with asynchr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23275
更新日期:2016-03-23 00:00:00
abstract::The spread of multidrug resistance via mobile genetic elements is a major clinical and veterinary concern. Pathogenic Escherichia coli harbour antibiotic resistance and virulence genes mainly on plasmids, but also bacteriophages and hybrid phage-like plasmids. In this study, the genomes of three E. coli phage-like pla...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-54895-4
更新日期:2019-12-11 00:00:00
abstract::Sleep, in addition to its brain restorative processes, plays an important role in memory transfer from its temporary store in the hippocampus to the more permanent storage in the neocortex. Alzheimer's disease (AD) affects memory and sleep. The aim of this study was to explore disturbances in global and local synchron...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41851-5
更新日期:2019-03-29 00:00:00
abstract::Bovine lactoferrin is a biglobular multifunctional iron binding glycoprotein that plays an important role in innate immunity against infections. We have previously demonstrated that selected peptides from bovine lactoferrin C-lobe are able to prevent both Influenza virus hemagglutination and cell infection. To deeper ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10492-x
更新日期:2017-09-06 00:00:00