Abstract：
:Adenoviruses are very efficient vectors for delivering therapeutic genes in preclinical and clinical trials. However, randomized controlled human trials have often been lacking clear clinically relevant results. We hypothesized that high lipid levels and specific lipoproteins could significantly decrease adenoviral transduction efficiency in vivo. Here we demonstrate that mice on a high fat diet have lower transgene expression compared to mice on a regular chow. In addition, on a high fat diet, ApoE-/- mice have much higher plasma transgene levels compared to LDLR-deficient mice. We also found that specific lipoprotein receptors play an important role in adenoviral transduction. These findings suggest that high plasma lipid levels, especially apoE-containing lipoproteins, reduce efficacy of adenoviral transduction in mice, which implies that high cholesterol levels in humans could be protective against viral infections and also lead to insufficient transgene expression in clinical trials using adenoviral vectors.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Kivelä AM,Huusko J,Gurzeler E,Laine A,Dijkstra MH,Dragneva G,Andersen CB,Moestrup SK,Ylä-Herttuala Sdoi
10.1038/s41598-017-00376-5subject
Has Abstractpub_date
2017-03-24 00:00:00pages
386issue
1issn
2045-2322pii
10.1038/s41598-017-00376-5journal_volume
7pub_type
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