Downregulation of the small GTPase SAR1A: a key event underlying alcohol-induced Golgi fragmentation in hepatocytes.

Abstract:

:The hepatic asialoglycoprotein receptor (ASGP-R) is posttranslationally modified in the Golgi en route to the plasma membrane, where it mediates clearance of desialylated serum glycoproteins. It is known that content of plasma membrane-associated ASGP-R is decreased after ethanol exposure, although the mechanisms remain elusive. Previously, we found that formation of compact Golgi requires dimerization of the largest Golgi matrix protein giantin. We hypothesize that ethanol-impaired giantin function may be related to altered trafficking of ASGP-R. Here we report that in HepG2 cells expressing alcohol dehydrogenase and hepatocytes of ethanol-fed rats, ethanol metabolism results in Golgi disorganization. This process is initiated by dysfunction of SAR1A GTPase followed by altered COPII vesicle formation and impaired Golgi delivery of the protein disulfide isomerase A3 (PDIA3), an enzyme that catalyzes giantin dimerization. Additionally, we show that SAR1A gene silencing in hepatocytes mimics the effect of ethanol: dedimerization of giantin, arresting PDIA3 in the endoplasmic reticulum (ER) and large-scale alterations in Golgi architecture. Ethanol-induced Golgi fission has no effect on ER-to-Golgi transportation of ASGP-R, however, it results in its deposition in cis-medial-, but not trans-Golgi. Thus, alcohol-induced deficiency in COPII vesicle formation predetermines Golgi fragmentation which, in turn, compromises the Golgi-to-plasma membrane transportation of ASGP-R.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Petrosyan A,Cheng PW,Clemens DL,Casey CA

doi

10.1038/srep17127

subject

Has Abstract

pub_date

2015-11-26 00:00:00

pages

17127

issn

2045-2322

pii

srep17127

journal_volume

5

pub_type

杂志文章
  • Spatiotemporal transcriptome provides insights into early fruit development of tomato (Solanum lycopersicum).

    abstract::Early fruit development is crucial for crop production in tomato. After fertilization, the ovary undergoes cell division and cell expansion before maturation. Although the roles of regulatory signals such as hormone and carbohydrate during early fruit development have been studied, the spatial distribution and the seq...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep23173

    authors: Zhang S,Xu M,Qiu Z,Wang K,Du Y,Gu L,Cui X

    更新日期:2016-03-18 00:00:00

  • Moonlighting transcriptional activation function of a fungal sulfur metabolism enzyme.

    abstract::Moonlighting proteins, including metabolic enzymes acting as transcription factors (TF), are present in a variety of organisms but have not been described in higher fungi so far. In a previous genome-wide analysis of the TF repertoire of the plant-symbiotic fungus Tuber melanosporum, we identified various enzymes, inc...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep25165

    authors: Levati E,Sartini S,Bolchi A,Ottonello S,Montanini B

    更新日期:2016-04-28 00:00:00

  • Low-flux scanning electron diffraction reveals substructures inside the ordered membrane domain.

    abstract::Ordered/disordered phase separation occurring in bio-membranes has piqued researchers' interest because these ordered domains, called lipid rafts, regulate important biological functions. The structure of the ordered domain has been examined with artificial membranes, which undergo macroscopic ordered/disordered phase...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-79083-7

    authors: Kinoshita M,Yamaguchi S,Matsumori N

    更新日期:2020-12-21 00:00:00

  • Deciphering molecular details in the assembly of alpha-type carboxysome.

    abstract::Bacterial microcompartments (BMCs) are promising natural protein structures for applications that require the segregation of certain metabolic functions or molecular species in a defined microenvironment. To understand how endogenous cargos are packaged inside the protein shell is key for using BMCs as nano-scale reac...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-33074-x

    authors: Liu Y,He X,Lim W,Mueller J,Lawrie J,Kramer L,Guo J,Niu W

    更新日期:2018-10-10 00:00:00

  • Complex rice systems to improve rice yield and yield stability in the face of variable weather conditions.

    abstract::Extreme weather events and pest outbreaks decrease rice yields and increase their variability, presenting challenges for the agricultural agenda to increase rice productivity and yield stability in Asia. The integration of azolla, fish and ducks has been shown to create robust systems that maintain high yields under h...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-32915-z

    authors: Khumairoh U,Lantinga EA,Schulte RPO,Suprayogo D,Groot JCJ

    更新日期:2018-10-03 00:00:00

  • Comparison of postoperative outcomes between patients with positive and negative straight leg raising tests who underwent full-endoscopic transforaminal lumbar discectomy.

    abstract::Full-endoscopic transforaminal lumbar discectomy (FETD) is increasingly used in patients with lumbar disc herniation (LDH). There is little knowledge on the related factors, including the straight leg raising test (SLR), that influence the operation. Consecutive patients with LDH who came to our hospital from August 2...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-73357-w

    authors: Wei FL,Gao H,Yan X,Yuan Y,Qian S,Gao Q,Guo S,Xue W,Qian J,Zhou C

    更新日期:2020-10-05 00:00:00

  • How goats avoid ingesting noxious insects while feeding.

    abstract::As mammalian herbivores feed, they often encounter noxious insects on plants. It is unknown how they handle such insects. We experimentally examined the behavioural responses of goats to the noxious spring-webworm (Ocnogyna loewii), and manipulated their sensory perception to reveal the process of insect detection. Go...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-14940-6

    authors: Berman TS,Ben-Ari M,Glasser TA,Gish M,Inbar M

    更新日期:2017-11-01 00:00:00

  • Genome-wide RAD sequencing data provide unprecedented resolution of the phylogeny of temperate bamboos (Poaceae: Bambusoideae).

    abstract::The temperate bamboos (tribe Arundinarieae, Poaceae) are strongly supported as monophyly in recent molecular studies, but taxonomic delineation and phylogenetic relationships within the tribe lack resolution. Here, we sampled 39 species (36 temperate bamboos and 3 outgroups) for restriction-site associated DNA sequenc...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-11367-x

    authors: Wang X,Ye X,Zhao L,Li D,Guo Z,Zhuang H

    更新日期:2017-09-14 00:00:00

  • Geochemistry and tectonic significance of late Paleoproterozoic A-type granites along the southern margin of the North China Craton.

    abstract::The Longwangzhuang pluton is a typical example of Paleoproterozoic A-type granite intrusions at the southern margin of the North China Craton. This pluton is composed of arfvedsonite granite and minor aegirine-augite granites. Samples from both granite types display similar zircon U-Pb ages with 207U-206Pb ages of 161...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-56820-1

    authors: Wang Y,Yang YZ,Siebel W,Zhang H,Zhang YS,Chen F

    更新日期:2020-01-09 00:00:00

  • Defect controlled magnetism in FeP/graphene/Ni(111).

    abstract::Spin switching of organometallic complexes by ferromagnetic surfaces is an important topic in the area of molecular nanospintronics. Moreover, graphene has been shown as a 2D surface for physisorption of molecular magnets and strain engineering on graphene can tune the spin state of an iron porphyrin (FeP) molecule fr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep03405

    authors: Bhandary S,Eriksson O,Sanyal B

    更新日期:2013-12-03 00:00:00

  • Proper direction of male genitalia is prerequisite for copulation in Drosophila, implying cooperative evolution between genitalia rotation and mating behavior.

    abstract::Animal morphology and behavior often appear to evolve cooperatively. However, it is difficult to assess how strictly these two traits depend on each other. The genitalia morphologies and courtship behaviors in insects, which vary widely, may be a good model for addressing this issue. In Diptera, phylogenetic analyses ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-36301-7

    authors: Inatomi M,Shin D,Lai YT,Matsuno K

    更新日期:2019-01-18 00:00:00

  • Effects of Killari earthquake on the paleo-channel of Tirna River Basin from Central India using anisotropy of magnetic susceptibility.

    abstract::The Killari Earthquake (Moment magnitude 6.1) of September 30, 1993, occurred in the state of Maharashtra, India, has an epicenter (18°03' N, 76°33' E) located at ~ 40 km SSW of Killari Town. The ~ 125 km long basin of Tirna River, close to the Killari Town, currently occupies the area that has witnessed episodic intr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-77542-9

    authors: Lakshmi BV,Deenadayalan K,Gawali PB,Misra S

    更新日期:2020-11-25 00:00:00

  • Wetland salinity induces sex-dependent carry-over effects on the individual performance of a long-distance migrant.

    abstract::Salinization is having a major impact on wetlands and its biota worldwide. Specifically, many migratory animals that rely on wetlands are increasingly exposed to elevated salinity on their nonbreeding grounds. Experimental evidence suggests that physiological challenges associated with increasing salinity may disrupt ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-07258-w

    authors: Masero JA,Abad-Gómez JM,Gutiérrez JS,Santiago-Quesada F,Senner NR,Sánchez-Guzmán JM,Piersma T,Schroeder J,Amat JA,Villegas A

    更新日期:2017-07-31 00:00:00

  • Repurposing Thioridazine (TDZ) as an anti-inflammatory agent.

    abstract::Nuclear factor-kB (NF-kB) is a crucial transcription factor in the signal transduction cascade of the inflammatory signaling. Activation of NF-κB depends on the phosphorylation of IκBα by IκB kinase (IKKβ) followed by subsequent ubiquitination and degradation. This leads to the nuclear translocation of the p50- p65 su...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-30763-5

    authors: Baig MS,Roy A,Saqib U,Rajpoot S,Srivastava M,Naim A,Liu D,Saluja R,Faisal SM,Pan Q,Turkowski K,Darwhekar GN,Savai R

    更新日期:2018-08-20 00:00:00

  • Longrange PCR-based next-generation sequencing in pharmacokinetics and pharmacodynamics study of propofol among patients under general anaesthesia.

    abstract::The individual response of patients to propofol results from the influence of genetic factors. However, the state of knowledge in this matter still remains insufficient. The aim of our study was to determine genetic predictors of variable pharmacokinetics and pharmacodynamics of propofol within selected 9 genes coding...

    journal_title:Scientific reports

    pub_type: 临床试验,杂志文章

    doi:10.1038/s41598-017-15657-2

    authors: Zakerska-Banaszak O,Skrzypczak-Zielinska M,Tamowicz B,Mikstacki A,Walczak M,Prendecki M,Dorszewska J,Pollak A,Lechowicz U,Oldak M,Huminska-Lisowska K,Molinska-Glura M,Szalata M,Slomski R

    更新日期:2017-11-13 00:00:00

  • Education in early life markedly reduces the probability of cognitive impairment in later life in Colombia.

    abstract::The objective of this study was to investigate associations between education in early life and cognitive impairment in later life in Colombia. Participants were community-dwelling adults aged 60 years or older from the National Study of Health, Wellbeing and Ageing. Trained interviewers administered a shorter version...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-74822-2

    authors: O'Donovan G,Hamer M,Sarmiento OL,Hessel P

    更新日期:2020-10-19 00:00:00

  • Identification of Methylation-Driven, Differentially Expressed STXBP6 as a Novel Biomarker in Lung Adenocarcinoma.

    abstract::DNA methylation is an essential epigenetic marker associated with the silencing of gene expression. Although various genome-wide studies revealed aberrantly methylated gene targets as molecular biomarkers for early detection, the survival rate of lung cancer patients is still poor. In order to identify methylation-dri...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep42573

    authors: Lenka G,Tsai MH,Lin HC,Hsiao JH,Lee YC,Lu TP,Lee JM,Hsu CP,Lai LC,Chuang EY

    更新日期:2017-02-15 00:00:00

  • The Safety Limits Of An Extended Fast: Lessons from a Non-Model Organism.

    abstract::While safety of fasting therapy is debated in humans, extended fasting occurs routinely and safely in wild animals. To do so, food deprived animals like breeding penguins anticipate the critical limit of fasting by resuming feeding. To date, however, no molecular indices of the physiological state that links spontaneo...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep39008

    authors: Bertile F,Fouillen L,Wasselin T,Maes P,Le Maho Y,Van Dorsselaer A,Raclot T

    更新日期:2016-12-19 00:00:00

  • Active dynamics of colloidal particles in time-varying laser speckle patterns.

    abstract::Colloidal particles immersed in a dynamic speckle pattern experience an optical force that fluctuates both in space and time. The resulting dynamics presents many interesting analogies with a broad class of non-equilibrium systems like: active colloids, self propelled microorganisms, transport in dynamical intracellul...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep27681

    authors: Bianchi S,Pruner R,Vizsnyiczai G,Maggi C,Di Leonardo R

    更新日期:2016-06-09 00:00:00

  • Boolean Models of Biological Processes Explain Cascade-Like Behavior.

    abstract::Biological networks play a key role in determining biological function and therefore, an understanding of their structure and dynamics is of central interest in systems biology. In Boolean models of such networks, the status of each molecule is either "on" or "off" and along with the molecules interact with each other...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep20067

    authors: Chen H,Wang G,Simha R,Du C,Zeng C

    更新日期:2016-01-29 00:00:00

  • Resolving new ultrastructural features of cytokinetic abscission with soft-X-ray cryo-tomography.

    abstract::Mammalian cytokinetic abscission is mediated by the ESCRT membrane fission machinery. While much has been clarified on the topology and kinetics of abscission through high-resolution microscopy, key questions regarding the mechanism of abscission remain open. Here we apply cryogenic soft-X-ray tomography to elucidate ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep27629

    authors: Sherman S,Kirchenbuechler D,Nachmias D,Tamir A,Werner S,Elbaum M,Elia N

    更新日期:2016-06-10 00:00:00

  • Substrate Specific Inhibitor Designed against the Immunomodulator GMF-beta Reversed the Experimental Autoimmune Encephalomyelitis.

    abstract::The concept of substrate inhibition to prevent its phosphorylation has potential in drug discovery and is envisioned to treat the autoimmune disorder multiple sclerosis (MS). Glia maturation factor-β (GMF-β) Ser83 phosphorylation by protein kinase A (PKA) is pivotal in the activation of GMF-β-p38MAPK-NFκB biochemical ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-60710-2

    authors: Vattathara JJ,Prakash O,Subhramanian S,Satheeshkumar MK,Xavier T,Anil M,Pillai GS,Anandakuttan A,Radhakrishnan S,Sivanarayanan TB,Akk U,Mohan CG,Menon KN

    更新日期:2020-03-02 00:00:00

  • Direct DNA and RNA detection from large volumes of whole human blood.

    abstract::PCR inhibitors in clinical specimens negatively affect the sensitivity of diagnostic PCR and RT-PCR or may even cause false-negative results. To overcome PCR inhibition, increase the sensitivity of the assays and simplify the detection protocols, simple methods based on quantitative nested real-time PCR and RT-PCR wer...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-21224-0

    authors: Cai D,Behrmann O,Hufert F,Dame G,Urban G

    更新日期:2018-02-21 00:00:00

  • Adaptive simulations, towards interactive protein-ligand modeling.

    abstract::Modeling the dynamic nature of protein-ligand binding with atomistic simulations is one of the main challenges in computational biophysics, with important implications in the drug design process. Although in the past few years hardware and software advances have significantly revamped the use of molecular simulations,...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-08445-5

    authors: Lecina D,Gilabert JF,Guallar V

    更新日期:2017-08-16 00:00:00

  • Haptoglobin polymorphism and prostate cancer mortality.

    abstract::Prostate cancer is a common malignancy in men worldwide and it is known that oxidative stress is a risk factor for cancer development. A common functional haptoglobin (Hp) polymorphism, originating from a duplication of a gene segment spanning over two exons, results in three distinct phenotypes with different anti-ox...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-69333-z

    authors: Kaiser M,Thurner EM,Mangge H,Herrmann M,Semeraro MD,Renner W,Langsenlehner T

    更新日期:2020-08-04 00:00:00

  • Natural infection by Procyrnea uncinipenis (Nematoda, Habronematidae), a parasite from rheas, an autoctone bird from South America, in emus Dromaius novaehollandiae, a ratite from New Zealand.

    abstract::The present study reports a natural infection of emus, Dromaius novaehollandiae, by the nematode Procyrnea uncinipenis. Five adult emus from a scientific breeding farm at North Fluminense State University located in the city of Campos dos Goytacazes, Rio de Janeiro state, Brazil were necropsied, and their gastrointest...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-73754-1

    authors: Ederli NB,Gallo SSM,de Oliveira FCR

    更新日期:2020-10-06 00:00:00

  • New Insight into Mixing Fluoride and Chloride in Bioactive Silicate Glasses.

    abstract::Adding fluoride into bioactive glasses leads to fluorapatite formation and a decrease in glass transition temperature. Recently, chloride has been introduced into glasses as an alternative to fluoride. The presence of the large chloride ion lowers glass crystallisation tendency and increases glass molar volume, which ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-19544-2

    authors: Chen X,Chen X,Pedone A,Apperley D,Hill RG,Karpukhina N

    更新日期:2018-01-22 00:00:00

  • Impact of an active lifestyle on heart rate variability and oxidative stress markers in offspring of hypertensives.

    abstract::Familial history of hypertension is associated with autonomic dysfunction and increase in blood pressure (BP). However, an active lifestyle has been found to improve a number of health outcomes and reduce all-cause mortality. The aim of the present study was to investigate the effects of an active lifestyle on hemodyn...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-69104-w

    authors: Santa-Rosa FA,Shimojo GL,Dias DS,Viana A,Lanza FC,Irigoyen MC,De Angelis K

    更新日期:2020-07-24 00:00:00

  • Identification and characterization of aspartyl-tRNA synthetase inhibitors against Mycobacterium tuberculosis by an integrated whole-cell target-based approach.

    abstract::Mycobacterium tuberculosis, the causative agent of tuberculosis, has surpassed HIV as the leading cause of death due to an infectious disease worldwide, being responsible for more than 1.5 million deaths in low-income countries. In response to a pandemic threat by drug resistant strains, the tuberculosis research comm...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-31157-3

    authors: Soto R,Perez-Herran E,Rodriguez B,Duma BM,Cacho-Izquierdo M,Mendoza-Losana A,Lelievre J,Aguirre DB,Ballell L,Cox LR,Alderwick LJ,Besra GS

    更新日期:2018-08-23 00:00:00

  • A computationally efficient hybrid 2D-3D subwoofer model.

    abstract::A subwoofer generates the lowest frequency range in loudspeaker systems. Subwoofers are used in audio systems for live concerts, movie theatres, home theatres, gaming consoles, cars, etc. During the last decades, numerical simulations have emerged as a cost- and time-efficient complement to traditional experiments in ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-80092-9

    authors: Bokhari AH,Berggren M,Noreland D,Wadbro E

    更新日期:2021-01-08 00:00:00