Abstract:
:Recently we determined that the Transient Receptor Potential Vanilloid 4 ion channel (TRPV4) has a crucial signaling role in a pathway that regulates various aspects of lens epithelium function. Here, we report on a different TRPV channel, TRPV1, in porcine lens. The presence of TRPV1 in the lens was evident from RT-PCR studies and Western blot analysis of MAPK signaling pathway activation caused by the TRPV1 agonist capsaicin. TRPV1 mRNA was detected in the epithelium of porcine as well as human lens. Transient ERK1/2 and p38 MAPK phosphorylation was detected within 1 min in the epithelium isolated from intact porcine lenses exposed to capsaicin (100 nM), a selective TRPV1 agonist, and the response was significantly inhibited by A889245 (1.0 μM), a TRPV1 antagonist. A similar ERK 1/2 and p38 response in the epithelium, also inhibitable by A889245, was evident in lenses treated with hyperosmotic solution (350 vs 300 mOsm). Lenses pre-treated with either the cytosolic Ca2+ chelator BAPTA-AM or the PKC inhibitor sotrastaurin (1.0 μM) had a diminished ERK1/2 activation response to capsaicin and hyperosmotic solution. Taken together the findings support the notion that TRPV1 functions as a plasma membrane ion channel that, when activated, permits the entry of extracellular calcium into the lens epithelium, leading to activation of PKC, ERK1/2 and p38 MAPK. It is significant that the findings confirm earlier proposals that hyperosmotic stress is linked to TRPV1 channel activation in the mouse lens. Further studies are ongoing to determine what functional changes are triggered by the TRPV1-linked signaling pathways and how they might relate to lens volume homeostasis.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Mandal A,Shahidullah M,Delamere NAdoi
10.1016/j.exer.2018.04.006subject
Has Abstractpub_date
2018-07-01 00:00:00pages
128-136eissn
0014-4835issn
1096-0007pii
S0014-4835(18)30057-5journal_volume
172pub_type
杂志文章abstract::PAX6-related Aniridia is a sight-threatening disease involving progression of secondary glaucoma and aniridia related keratopathy (ARK). Change or loss of limbal epithelial progenitors causes epithelial surface defects. We analyzed the effect of PAX6 on mRNA expression changes with a two-step approach, as follows. Fir...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.10.002
更新日期:2019-02-01 00:00:00
abstract::To facilitate the peeling of internal limiting membrane or epiretinal membrane in vitreoretinal surgery, trypan blue (TB) and indocyanine green (ICG) have been used. However, the cytotoxicity of these dyes have been concerned. The aim of this study was to investigate the neurotoxic effects of TB on rat retinal ganglio...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.02.010
更新日期:2005-10-01 00:00:00
abstract::The purpose of this study was to investigate the influence of hyperoxia and hypercapnia on fundus pulsations in the macular and optic disc region and determine a potential autoregulative capacity of the choroid under these conditions. In a randomized cross-over study 100% O2 and a mixture of 5% CO2 with air was inhale...
journal_title:Experimental eye research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0014-4835(05)80019-3
更新日期:1995-12-01 00:00:00
abstract::Eleven monoclonal antibodies against different segments of bovine rhodopsin were used with immunogold-dextran markers to label Lowicryl thin sections of bovine and frog retinal photoreceptor cells for visualization by transmission electron microscopy. Antibodies against the C-terminus, F1-F2 loop and N-terminus of rho...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(86)90017-5
更新日期:1986-01-01 00:00:00
abstract::The transmembrane glycoprotein CD44 is a cell adhesion molecule which has recently been localized in a variety of cell types. It mediates cell attachment to extracellular matrix components and also binds to the actin cytoskeleton within the cell. In this study, the presence and distribution of CD44 in the mouse retina...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1026
更新日期:1994-03-01 00:00:00
abstract::The influence of autonomic neurotransmitters on the growth and melanogenesis of cultured uveal melanocytes was studied. Uveal melanocytes were cultured with medium supplemented with cAMP elevating agents and basic fibroblast growth factor (complete medium). The cells were plated into multiple well plates, and various ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2000.0869
更新日期:2000-09-01 00:00:00
abstract::A number of deep-sea fish have bright yellow lenses whose coloration is attributable to a variety of largely unidentified short-wave absorbing pigments. Here the pigment of the deep-sea fish Stylephorus chordatus has been isolated and identified by NMR and mass spectroscopy as kynurenine; a pigment also found in the h...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(92)90091-6
更新日期:1992-07-01 00:00:00
abstract::Mutations in the optic atrophy type 1 (OPA1) gene give rise to human autosomal dominant optic atrophy. The purpose of this study is to investigate OPA1 protein expression in the human retina and optic nerve. A rabbit polyclonal antiserum was generated using a fusion protein covering amino acids 647 to 808 of the human...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.06.004
更新日期:2006-11-01 00:00:00
abstract::Stroboscopic luminance flicker has been found to prevent the increase in eye growth normally associated with form deprivation through the release of retinal dopamine. In this study, we sought to investigate whether dopamine plays a role in the decreased growth observed with 2 Hz sine-wave luminance flicker and increas...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.107822
更新日期:2019-12-01 00:00:00
abstract::Human retinal pigmented epithelial (RPE) cells cultured on plastic, unlike RPE in situ, often fail to density arrest, do not produce melanin, and do not express mRNA for cellular retinaldehyde binding protein (CRALBP). When human RPE are cultured on the extracellular matrix produced by bovine corneal endothelial cells...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1158
更新日期:1993-11-01 00:00:00
abstract::Laser Doppler flowmetry (LDF) measures the flux of red blood cells in a vascular network embedded in tissue. In the present report, choroidal and ciliary body blood flow was measured continuously using an LDF technique in pentobarbital anesthetized cats. Blood flow measurements were made from the choroid beneath the l...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(91)90068-p
更新日期:1991-08-01 00:00:00
abstract::The purpose of the current study was to localize the lens membrane protein, MIP 26, in nuclear fiber cells from different regions of aged normal and age-related cataractous human lenses. Adult, juvenile, fetal and embryonic nuclear regions in aged normal and age-related nuclear cataractous lenses were morphologically ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0579
更新日期:1999-01-01 00:00:00
abstract::Pseudoexfoliation syndrome (PEX) may lead to the development of pseudoexfoliative glaucoma (PEG), a potential cause of irreversible blindness, if left untreated. This type of glaucoma often presents with much higher intraocular pressure (IOP) values than observed in primary open angle glaucoma, and patients are often ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.107837
更新日期:2019-12-01 00:00:00
abstract::Addition of substance P (10(-7) to 10(-6) M) to rabbit iris sphincter muscle induced: (a) a rapid phosphodiesteratic breakdown of phosphatidylinositol 4,5-bisphosphate (PIP2) into 1,2-diacylglycerol (DG), measured as phosphatidic acid, and inositol triphosphate (IP3), measured by anion-exchange chromatography; (b) a r...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(86)80089-6
更新日期:1986-08-01 00:00:00
abstract::Beta-crystallins are major protein constituents of the mammalian lens, where their stability and association into higher order complexes are critical for lens clarity and refraction. Dimerization is an initial step in formation of beta-crystallin complexes. Beta-crystallin association into dimers is energetically high...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2004.06.011
更新日期:2004-09-01 00:00:00
abstract::VEGF secretion by the human retinal pigment epithelium (hRPE) plays an important role in retinal and choroidal neovascularization. In this study, transforming growth factor-beta2 (TGF-beta2)-induced vascular endothelial growth factor (VEGF) gene expression was investigated in hRPE cells. Treatment of hRPE cells with T...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.12.016
更新日期:2007-05-01 00:00:00
abstract::This review is focused on iron metabolism in the retina and in the lens and its relation to their respective age-related pathologies, macular degeneration (AMD) and cataract (ARC). Several aspects of iron homeostasis are considered first in the retina and second in the lens, paying particular attention to the transpor...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2010.03.003
更新日期:2010-06-01 00:00:00
abstract::Retinal lesions in the posterior pole of laboratory mice occur due to native, developmental abnormalities or as a consequence of environmental or experimental conditions. In this study, we investigated the rate and extent of retinal lesions as a result of prolonged ocular exposure following general anesthesia. Followi...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.05.012
更新日期:2019-08-01 00:00:00
abstract::Type I and type II isoenzymes of cyclic AMP-dependent protein kinase were identified by anion exchange chromatography and immunoblotting in human retina at various stages of development. The type I form was typical of the early fetal stage during which proliferation and migration were the primary cellular processes. I...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(89)90059-6
更新日期:1989-06-01 00:00:00
abstract::The effect of dietary supplementation with acetylsalicylic acid on the increased modification, and susceptibility to modification, of lens crystallins from the streptozocin diabetic rat, has been determined. This was done by the measurement of characteristic markers of protein post-translational oxidative modification...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1186
更新日期:1993-12-01 00:00:00
abstract::The interaction of keratocytes with extracellular matrix components plays an important role in the maintenance of corneal transparency and shape as well as in the healing of corneal wounds. In particular, the interaction of these cells with collagen and cell-mediated collagen contraction contribute to wound closure. E...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.107933
更新日期:2020-02-01 00:00:00
abstract::Contact inhibition is an important mechanism for maintaining corneal endothelium in a non-replicative state. Protein tyrosine phosphatases (PTPs) play a role in regulating the integrity of cell-cell contacts, differentiation, and growth. In this study, we aimed to evaluate whether phosphatases are involved in the main...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.03.015
更新日期:2005-11-01 00:00:00
abstract::This study investigated the changes in pH during retinal artery occlusion by means of extracellular H+ concentration ([H+]o) measurements in the retina under both air and 100% O2 ventilation. Occlusion was produced in intact anesthetised cats by pressing with a probe onto a retinal artery. [H+]o profiles were recorded...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.11.002
更新日期:2005-04-01 00:00:00
abstract::The suitability of the rat derived SV-40T immortalized RPE-J cell line for identifying proteome changes associated with RPE differentiation was evaluated by surveying changes in protein expression levels. Rat RPE-J cells were induced to undergo differentiation in culture by growth at the nonpermissive temperature of 4...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2001.1058
更新日期:2001-10-01 00:00:00
abstract::Experiments were performed using [1-14C]PGH2 endoperoxide as substrate to assess which, if any, of the PGH2 utilizing enzymes were present in rat lens microsomes. After a 15 min incubation, prostaglandins (PGs) were extracted, separated by thin-layer chromatography and labeled PGs located by autoradiography. The bands...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80010-6
更新日期:1987-02-01 00:00:00
abstract::Autoradiographic studies showed selective accumulation of [3H]-glycine, [3H]-GABA, and the GABA agonist [3H]-muscimol by neurons of the mudpuppy retina. [3H]-Glycine was taken up by bipolar cells, amacrine cells, and displaced amacrine or ganglion cells. Both [3H]-GABA and [3H]-muscimol were also accumulated by bipola...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90116-7
更新日期:1984-07-01 00:00:00
abstract::The purpose of this study was to assess the effect of taurine and apical potassium concentration modelling in vivo light evoked changes on the transepithelial potential (TEP) and the transepithelial resistance (TER) of isolated bovine retinal pigment epithelium (RPE). Isolated specimens of bovine non-tapetal RPE-Bruch...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.06.020
更新日期:2006-02-01 00:00:00
abstract::The nature of glucose transport at the microvascular blood-retinal barrier was studied using primary cultures of microvascular endothelial cells from bovine retina. Uptake of 3-O-methyl-D-glucose (3MG), a non-metabolizable glucose analogue, was rapid and equilibrative. 3MG uptake could be inhibited by traditional gluc...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(83)90011-8
更新日期:1983-02-01 00:00:00
abstract::Basic fibroblast growth factor (bFGF) has been shown to rescue dying photoreceptor cells in the RCS rat, a model with a genetic defect of the RPE that impairs outer segment phagocytosis. The purpose of the present study was to determine whether intravitreal injection of bFGF would have a similar effect on photorecepto...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0147
更新日期:1996-11-01 00:00:00
abstract::The effects of temperature on rod outer segment (ROS) length and membrane shedding were studied in a cyprinodont fish, Fundulus zebrinus. After 30 days in 14L/10D cyclic light and 17 degrees C, ROS length averaged 41.2 microns. Fish were then exposed to 7, 17 or 27 degrees C for 10 and 25 days before being sampled 5 h...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(05)80036-3
更新日期:1995-08-01 00:00:00