Abstract:
:Basic fibroblast growth factor (bFGF) has been shown to rescue dying photoreceptor cells in the RCS rat, a model with a genetic defect of the RPE that impairs outer segment phagocytosis. The purpose of the present study was to determine whether intravitreal injection of bFGF would have a similar effect on photoreceptor cell death in the vitiligo (C57BL/6-mi(vit)/mi(vit)) mouse. This mutant mouse loses photoreceptor cells slowly over many months. Experimental evidence suggests that the primary cellular site of the defect is the RPE. In the present study, bFGF was prepared with and without heparin in PBS and injected intravitreally into vitiligo mice at ages 2, 4, 6, 8 and 13 weeks, surrounding the onset of photoreceptor cell death. Non-injected, PBS-injected and heparin/PBS injected mice served as controls. Scotopic ERG's were performed on one group of mice prior to killing. Mice were killed 4, 6 or 10 weeks following the injection and the eyes were processed for histology and analysed. The amplitude of the b-wave was significantly smaller in mice injected with bFGF/PBS than in PBS-injected and non-injected eyes regardless of the time of injection or duration following injection. Histological examination revealed that the number of rows of photoreceptor cells did not differ significantly between bFGF-injected, vehicle- or non-injected mice. Although slight improvement in the attachment of outer segments to RPE was observed in 4 week mutants injected with bFGF/heparin/PBS, a similar result was obtained in heparin/PBS injected mutants. In mice injected with bFGF without heparin, detachment was severe and gross disruption of neural retina was observed. There were significantly more macrophages and photoreceptor cells in the subretinal space in bFGF injected mice. It appears that at the dosages and times administered, bFGF does not improve the electrophysiological functioning of the retina nor retard the degeneration of the retina in the vitiligo mouse as it does in the RCS rat.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Smith SB,Titelman R,Hamasaki DIdoi
10.1006/exer.1996.0147subject
Has Abstractpub_date
1996-11-01 00:00:00pages
565-77issue
5eissn
0014-4835issn
1096-0007pii
S0014-4835(96)90147-5journal_volume
63pub_type
杂志文章abstract::The pink-eyed dilution (p) locus is known to control the quantity of melanin pigment made within melanocytes and retinal pigment epithelium (RPE) in the eye. We have examined the effects of several mutant allele combinations at the murine p locus on the number and morphology of melanosomes in choroidal melanocytes and...
journal_title:Experimental eye research
pub_type: 杂志文章
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0265
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2018.04.006
更新日期:2018-07-01 00:00:00
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2005.11.013
更新日期:2006-07-01 00:00:00
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journal_title:Experimental eye research
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doi:10.1016/0014-4835(90)90092-9
更新日期:1990-11-01 00:00:00
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doi:10.1016/0014-4835(83)90138-0
更新日期:1983-12-01 00:00:00
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journal_title:Experimental eye research
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doi:10.1006/exer.2001.1007
更新日期:2001-07-01 00:00:00
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2020.108401
更新日期:2020-12-14 00:00:00
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pub_type: 杂志文章,评审
doi:10.1016/j.exer.2013.09.015
更新日期:2014-09-01 00:00:00
abstract::Injection of herpes simplex virus vectors into the vitreous of primate eyes induces an acute, transient uveitis. The purpose of this study was to characterize innate immune responses of macaque neural retina tissue to the herpes simplex virus type 1-based gene delivery vector hrR3. PCR array analysis demonstrated the ...
journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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更新日期:2017-05-01 00:00:00
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doi:10.1006/exer.2001.1115
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journal_title:Experimental eye research
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abstract::Prostaglandins are widely used to lower intraocular pressure (IOP) as part of the treatment regimen for glaucoma. While FP and EP2 agonists are known to lower IOP, we investigated the ocular hypotensive activity and ocular drug distribution of PF-04475270, a novel EP4 agonist following topical administration in normot...
journal_title:Experimental eye research
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doi:10.1016/j.exer.2009.05.002
更新日期:2009-11-01 00:00:00
abstract::Transport of circulating sulfur amino acids (SAA) into the lens epithelium and de novo glutathione (GSH) synthesis were studied in the perfused guinea-pig eye. Plasma-to-aqueous transfer of SAA was in their intact form (> or = 98%) and comparable with sucrose (an extracellular marker) within 30 min. The unidirectional...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0260
更新日期:1997-04-01 00:00:00
abstract::We investigated the pharmacological actions of hydrogen sulfide (H(2)S) using sodium hydrosulfide (NaHS) and sodium sulfide (Na(2)S) as donors on isolated porcine irides in the presence of tone induced by muscarinic receptor stimulation. Furthermore, we also investigated the mechanism of action of H(2)S in this smooth...
journal_title:Experimental eye research
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doi:10.1016/j.exer.2008.09.011
更新日期:2008-12-01 00:00:00
abstract::Structure and function of the retina mainly rely on its fatty acid (FA) composition. Evidence from epidemiological studies and from animal experiments indicates that FA composition of the retina is influenced by the diet. Mice under chronic high-fat diet (HFD) develop metabolic syndrome, a risk factor for diabetes tha...
journal_title:Experimental eye research
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abstract::The supply of L-carnosine, a bioactive dipeptide of β-alanine and l-histidine, to the retina across the blood-retinal barrier (BRB) was studied. The in vivo and in vitro studies revealed low uptake activities for [(3)H]Gly-Sar, a representative dipeptide, suggesting that l-carnosine transport plays only a minor role a...
journal_title:Experimental eye research
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doi:10.1016/j.exer.2013.06.002
更新日期:2013-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
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abstract::The transmembrane glycoprotein CD44 is a cell adhesion molecule which has recently been localized in a variety of cell types. It mediates cell attachment to extracellular matrix components and also binds to the actin cytoskeleton within the cell. In this study, the presence and distribution of CD44 in the mouse retina...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1026
更新日期:1994-03-01 00:00:00