Abstract:
:Mechanical failure of the ciliary zonule characterizes several ocular and systemic diseases. The mouse has emerged as a useful model system to investigate the composition and structure/function relationships of the zonule. However, visualizing the organization of the diaphanous fibers that comprise the zonule is technically challenging because the fibers do not take up conventional histological stains and are disrupted easily during processing. Here, we describe a simple method for maintaining physiological pressure within the mouse eye during fixation, and a gel-embedding technique for stabilizing the zonular fibers during subsequent tissue processing and imaging steps. This approach facilitates quantitative measurements of fiber number and cross-sectional dimensions and will allow the effects of targeted disruption of zonule components to be assessed systematically.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Bassnett Sdoi
10.1016/j.exer.2019.05.025subject
Has Abstractpub_date
2019-08-01 00:00:00pages
107685eissn
0014-4835issn
1096-0007pii
S0014-4835(19)30356-2journal_volume
185pub_type
杂志文章abstract::To facilitate the peeling of internal limiting membrane or epiretinal membrane in vitreoretinal surgery, trypan blue (TB) and indocyanine green (ICG) have been used. However, the cytotoxicity of these dyes have been concerned. The aim of this study was to investigate the neurotoxic effects of TB on rat retinal ganglio...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.02.010
更新日期:2005-10-01 00:00:00
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journal_title:Experimental eye research
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:1986-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2014.07.002
更新日期:2014-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0560
更新日期:1998-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2014.05.010
更新日期:2014-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80002-7
更新日期:1987-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2006-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2014-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1085
更新日期:1993-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2013-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2021-01-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.02.011
更新日期:2006-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80047-7
更新日期:1987-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.exer.2013.06.023
更新日期:2013-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0464
更新日期:1998-04-01 00:00:00
abstract::Fibroblast growth factors play a key role in regulating lens epithelial cell proliferation and differentiation via an anteroposterior gradient that exists between the aqueous and vitreous humours. FGF-2 is the most important for lens epithelial cell proliferation and differentiation. It has been proposed that the pres...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.12.004
更新日期:2014-03-01 00:00:00
abstract:PURPOSE:To investigate the potential protective effect of novel G protein coupled estrogen receptor (GPER1) against the neurotoxicity induced by NMDA in the mouse retina. METHODS:We induce retinal ganglion cells (RGCs) toxic injury through intravitreal injection of NMDA or acute ocular hypertension (AOH) induced by an...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.107826
更新日期:2019-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2011.09.022
更新日期:2011-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0512
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.03.021
更新日期:2005-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.10.010
更新日期:2019-02-01 00:00:00
abstract::Previous evidence has shown clearly that sugar cataract formation results from unusually high intracellular levels of polyol. Documentation of polyol-related histological changes in the cortical fiber cells of the equatorial zone has been extensive. However, little attention has been given to the early changes in the ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90107-6
更新日期:1990-06-01 00:00:00
abstract::Thymic stromal lymphopoietin (TSLP) is an interleukin 7 (IL-7)-like four helix bundle cytokine that plays diverse roles in the regulation of immune responses. In fungal infection, pattern recognition receptors (PRRs), including the cell surface Toll-like receptors (TLRs) and cytoplasmic NOD-like receptors, recognize p...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.02.020
更新日期:2019-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::A panel of anti-rhodopsin monoclonal antibodies (MAbs) of defined epitope specificity has been evaluated by immunocytochemistry. Most of the IgG class MAbs (23/27) gave positive results, but only a few of the IgM class MAbs (2/21) were useful for this application. MAbs specific to the N-terminal region stained rod out...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(89)80022-3
更新日期:1989-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
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更新日期:2017-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2012.12.016
更新日期:2013-04-01 00:00:00
abstract::Excised bovine and rhesus monkey vitreous samples were subjected to different hydrostatic pressures (0.03, 1 and 2 atm) for 12 hr. At the end of the experiment the samples were frozen in a -70 degrees C dry ice-acetone bath and thereafter maintained in frozen conditions. Differential scanning calorimetry (DSC) was use...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2001.1021
更新日期:2001-07-01 00:00:00