当前位置： SCI文献检索 > EXPERIMENTAL EYE RESEARCH期刊下所有文献 > Effects of norepinephrine and 5-hydroxytryptamine on phosphoinositide-PO4 turnover in rabbit cornea.

Effects of norepinephrine and 5-hydroxytryptamine on phosphoinositide-PO4 turnover in rabbit cornea.

Abstract：

:We have investigated: (a) Phospholipid composition and phosphoinositide-PO4 turnover in rabbit cornea tissues; and (b) the effects of adrenergic and serotonergic agonists on breakdown of phosphoinositides in the rabbit cornea. The data obtained from these studies can be summarized as follows: (1) in the cornea phosphatidylcholine and phosphatidylethanolamine constitute about 55%, phosphatidylinositol (PI) 10%, and phosphatidylinositol 4-phosphate (PIP), phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid (PA) comprise about 1% each of the total phospholipids; (2) incubation of cornea in 32Pi-containing medium resulted in incorporation of radioactivity in tissue phospholipids. The radioactivity was highest in PIP2 (39%), followed by PI (19%), PIP (16%) and PA (5% of the total radioactivity). When compared with stroma and endothelium, the cornea epithelium was most active in phosphoinositide metabolism; (3) addition of norepinephrine (NE) or 5-hydroxytryptamine (5-HT), 200 microM each, to 32P-labeled cornea resulted in a loss of radioactivity in PIP and PIP2 by about 12- and 20%, respectively. Concomitantly, the radioactivity in PA and PI was increased by 44- and 66%, respectively. The effects of the neurotransmitters were time- and concentration-dependent. When added to the cornea labeled with myo [3H] inositol, NE and 5-HT increased the production of labeled myo-inositol phosphates; (4) prazosin (20 microM), but not yohimbine or propranolol, blocked the effects of NE. Similarly, the effects of 5-HT were antagonized by methysergide (20 microM) and ketanserin (10 microM) but not by prazosin. These data demonstrate that NE and 5-HT stimulate phospholipase C-mediated hydrolysis of PIP2 into diacylglycerol (DG) and myo-inositol trisphosphate (IP3). Furthermore, the effects of NE and 5-HT are mediated by alpha 1-adrenergic and 5-HT2 receptors, respectively. It is suggested that IP3, by releasing Ca2+ from ER, and DG, by activating protein kinase C, may function as second-messenger molecules which may participate in agonist-induced functional responses, including chloride transport, in the cornea epithelium.

journal_name

Exp Eye Res

journal_title

Experimental eye research

authors

Akhtar RA

doi

10.1016/s0014-4835(87)80047-7

subject

Has Abstract

pub_date

1987-06-01 00:00:00

pages

849-62

issue

6

eissn

0014-4835

issn

1096-0007

journal_volume

44

pub_type

杂志文章

相关文献

EXPERIMENTAL EYE RESEARCH文献大全
  • Selective solubilization of two populations of polypeptides from bovine retinal basement membranes.

    abstract：:Basement membranes isolated from the entire neural retinae of normal cows consist of a heterogeneous mixture of polypeptides resolvable as two major populations, one collagenous and the other non-collagenous. The non-collagenous population consists of disulfide-bonded aggregates of which the major fraction is soluble ...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/0014-4835(83)90010-6

    authors： Duhamel RC,Meezan E,Brendel K

    更新日期：1983-02-01 00:00:00

  • Pressures in the juxtacanalicular tissue and Schlemm's canal in monkeys.

    abstract：:A micropuncture technique involving the use of microcannulas with tip diameters less than 5 microns was used to measure the pressure in Schlemm's canal and in the meshwork at distances approximately 7 and 14 microns from the inner wall of Schlemm's canal. In one set of experiments where the spontaneous intraocular pre...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/0014-4835(92)90151-h

    authors： Mäepea O,Bill A

    更新日期：1992-06-01 00:00:00

  • Simvastatin induces caspase-dependent apoptosis and activates P53 in OCM-1 cells.

    abstract：:Simvastatin is a cholesterol-lowering drug which exhibits numerous pleiotropic effects including anti-cancer activity. Yet, the anti-cancer effects in choroidal melanoma remain poorly characterized. Therefore, in this study, we investigated the effects of simvastatin on OCM-1 cells growth, apoptosis and cycle. Simvast...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/j.exer.2013.05.013

    authors： Wang Y,Xu SL,Wu YZ,Zhao MS,Xu WJ,Yang HY,Li YX

    更新日期：2013-08-01 00:00:00

  • Inhibition of experimental choroidal neovascularization in mice by anti-VEGFA/VEGFR2 or non-specific siRNA.

    abstract：:Choroidal neovascularization (CNV) is one of the severe pathological consequences of the end-stage of age-related macular degeneration (AMD). Several lines of evidence implicate increased levels of vascular endothelial growth factor (VEGF) in the retinas of AMD patients. Current available agents for the inhibition of ...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/j.exer.2010.06.019

    authors： Gu L,Chen H,Tuo J,Gao X,Chen L

    更新日期：2010-09-01 00:00:00

  • Immunohistological localization of calmodulin in feline rod outer segments.

    abstract：:The calcium-binding regulatory protein, calmodulin, was immunohistologically examined in feline rod outer segments. The peroxidase antiperoxidase technique was used for the immunohistological analysis and protein A-gold, to localize calmodulin electron-microscopically. Calmodulin was found to be located in the distal ...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/s0014-4835(87)80178-1

    authors： Wakakura M,Yamamoto N

    更新日期：1987-03-01 00:00:00

  • The effect of roscovitine on herpetic keratitis.

    abstract：:To determine the effect of roscovitine, a potent antiviral in tissue culture administered intramuscularly to rabbits or by eye drops to mice for the treatment of herpetic keratitis this study was commenced.New Zealand white rabbits infected with McKrae strain herpesvirus (HSV-1) were treated twice a day with 10mg Rosc...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/s0014-4835(03)00056-3

    authors： Avunduk AM,Varnell ED,Kaufman HE

    更新日期：2003-06-01 00:00:00

  • Changes in the localization and content of opsin during retinal development in the rds mutant mouse: immunocytochemistry and immunoassay.

    abstract：:Electron-microscope immunocytochemistry and antibody staining of nitrocellulose replicas of SDS gels (Western blots) were used in a developmental study to detect the presence and localization of opsin in the developing photoreceptors of rds (020/A) mutant mice and their BALB/c normal controls. Western blot analysis of...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/s0014-4835(87)80061-1

    authors： Usukura J,Bok D

    更新日期：1987-10-01 00:00:00

  • Kv3.3 potassium channels in lens epithelium and corneal endothelium.

    abstract：:Human Kv3.3/KCNC3 is a Shaw-type potassium channel that has been mapped to chromosome 19q13.3-13.4. Complete mouse and rat Kv3.3 cDNA coding sequences have been published, yet the human Kv3.3 cDNA has remained incomplete for years. We report here for the first time the amino acid sequence for hKv3.3 and the electrophy...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1006/exer.1999.0796

    authors： Rae JL,Shepard AR

    更新日期：2000-03-01 00:00:00

  • Corneal hyaluronan content during post-ablation healing: evidence for a transient depth-dependent contralateral effect.

    abstract：:The hyaluronan content during wound healing following excimer laser photoablation was investigated. Rabbit corneas were photoablated (Summit Omnimed, 193 nm, 5 mm diameter, 50 microns and 100 microns depth). Central optical zones of photoablated and contralateral corneas were removed 7, 30 and 90 days after surgery. C...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1006/exer.1996.0212

    authors： Drubaix I,Legeais JM,Robert L,Renard G

    更新日期：1997-03-01 00:00:00

  • Proliferative capacity of corneal endothelial cells.

    abstract：:The corneal endothelial monolayer helps maintain corneal transparency through its barrier and ionic "pump" functions. This transparency function can become compromised, resulting in a critical loss in endothelial cell density (ECD), corneal edema, bullous keratopathy, and loss of visual acuity. Although penetrating ke...

    journal_title：Experimental eye research

    pub_type： 杂志文章,评审

    doi：10.1016/j.exer.2011.08.014

    authors： Joyce NC

    更新日期：2012-02-01 00:00:00

  • Multifocal retinopathy in Dachshunds with CLN2 neuronal ceroid lipofuscinosis.

    abstract：:The CLN2 form of neuronal ceroid lipofuscinosis is an autosomal recessively inherited lysosomal storage disease that is characterized by progressive vision loss culminating in blindness, cognitive and motor decline, neurodegeneration, and premature death. CLN2 disease results from mutations in the gene that encodes th...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/j.exer.2015.02.012

    authors： Whiting RE,Pearce JW,Castaner LJ,Jensen CA,Katz RJ,Gilliam DH,Katz ML

    更新日期：2015-05-01 00:00:00

  • Mapping age-related elasticity changes in porcine lenses using bubble-based acoustic radiation force.

    abstract：:Bubble-based acoustic radiation force aims to measure highly localized tissue viscoelastic properties. In the current investigation, acoustic radiation force was applied to laser-induced bubbles to measure age-related changes in the spatial distribution of elastic properties within in vitro porcine lenses. A potential...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/j.exer.2006.10.007

    authors： Erpelding TN,Hollman KW,O'Donnell M

    更新日期：2007-02-01 00:00:00

  • Epidermal growth factor receptor transactivation by the cannabinoid receptor (CB1) and transient receptor potential vanilloid 1 (TRPV1) induces differential responses in corneal epithelial cells.

    abstract：:Corneal epithelial injury induces release of endogenous metabolites that are cannabinoid receptor 1 (CB1) and transient receptor potential vanilloid 1 (TRPV1) agonists. We determined the functional contributions by CB1 and TRPV1 activation to eliciting responses underlying wound healing in human corneal epithelial cel...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/j.exer.2010.06.022

    authors： Yang H,Wang Z,Capó-Aponte JE,Zhang F,Pan Z,Reinach PS

    更新日期：2010-09-01 00:00:00

  • The distribution of Na+,K(+)-ATPase in the retinal pigmented epithelium from chicken embryo is polarized in vivo but not in primary cell culture.

    abstract：:The polarity of retinal pigmented epithelia (RPE) from chicken embryos was studied in primary cell culture. Since cultured RPE approximates the morphological polarity of RPE in vivo, we investigated whether this polarity extends to the distribution of plasma membrane proteins that are peculiar to RPE. In contrast to o...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/0014-4835(90)90156-o

    authors： Rizzolo LJ

    更新日期：1990-10-01 00:00:00

  • Presence of Langerhans cells in the central cornea linked to the development of ocular herpes in mice.

    abstract：:Recurrences of herpetic stromal keratitis are believed to be initiated by reactivation of herpes simplex virus infection, probably in the trigeminal ganglion. Genetic features of the virus and the host as well as the immune status of the host influence the outcome of infection. Following infection on the snout with HS...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/0014-4835(92)90146-j

    authors： Jager MJ,Bradley D,Atherton S,Streilein JW

    更新日期：1992-06-01 00:00:00

  • Multiple, parallel cellular suicide mechanisms participate in photoreceptor cell death.

    abstract：:Photoreceptor degeneration in human photoreceptor dystrophies and in the relevant animal models has been thought to be executed by one common mechanism -- caspase-mediated apoptosis. However, recent experiments have challenged this concept. In previous experiments, analyzing gene expression in the degenerating rd/rd m...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/j.exer.2006.01.014

    authors： Lohr HR,Kuntchithapautham K,Sharma AK,Rohrer B

    更新日期：2006-08-01 00:00:00

  • Isolation of alpha-crystallin and its subunits by affinity chromatography on immobilized monoclonal antibodies.

    abstract：:Use has been made of the specific interactions between monoclonal antibodies and the alpha A or alpha B subunits of alpha-crystallin to devise methods for the purification of the intact protein or its subunits. alpha A and alpha B subunits were separated by affinity chromatography on an immobilized monoclonal antibody...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/s0014-4835(88)80007-1

    authors： Stevens A,Augusteyn RC

    更新日期：1988-04-01 00:00:00

  • Intravitreal controlled release of dexamethasone from engineered microparticles of porous silicon dioxide.

    abstract：:Dexamethasone is a glucocorticoid that is widely used in the ophthalmic arena. The recent FDA approved dexamethasone implant can provide a three month efficacy but with high rate of drug related cataract and high intraocular pressure (IOP). It seems that higher steroid in aqueous humor and around lens may be associate...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/j.exer.2014.11.002

    authors： Wang C,Hou H,Nan K,Sailor MJ,Freeman WR,Cheng L

    更新日期：2014-12-01 00:00:00

  • Increased susceptibility to metal catalysed oxidation of diabetic lens beta L crystallin: possible protection by dietary supplementation with acetylsalicylic acid.

    abstract：:The effect of dietary supplementation with acetylsalicylic acid on the increased modification, and susceptibility to modification, of lens crystallins from the streptozocin diabetic rat, has been determined. This was done by the measurement of characteristic markers of protein post-translational oxidative modification...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1006/exer.1993.1186

    authors： Jones RH,Hothersall JS

    更新日期：1993-12-01 00:00:00

  • TRPV1-dependent ERK1/2 activation in porcine lens epithelium.

    abstract：:Recently we determined that the Transient Receptor Potential Vanilloid 4 ion channel (TRPV4) has a crucial signaling role in a pathway that regulates various aspects of lens epithelium function. Here, we report on a different TRPV channel, TRPV1, in porcine lens. The presence of TRPV1 in the lens was evident from RT-P...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/j.exer.2018.04.006

    authors： Mandal A,Shahidullah M,Delamere NA

    更新日期：2018-07-01 00:00:00

  • Elevation of glucose in ocular compartments of developing chick embryos with glucocorticoid-induced cataract.

    abstract：:When 15-day-old developing chick embryos were administered hydrocortisone hemisuccinate sodium (HC:0.25 mumol per egg) the concentration of glucose in both the aqueous humor and the vitreous body began to increase significantly after 3 hr and reached 24.4 +/- 0.3 mM and 22.3 +/- 0.9 mM, respectively, at 48 hr. Thereaf...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/s0014-4835(89)80050-8

    authors： Nishigori H,Lee JW,Yamauchi Y,Iwatsuru M

    更新日期：1989-10-01 00:00:00

  • Altered expression of genes functioning in lipid homeostasis is associated with lipid deposition in NOD mouse lacrimal gland.

    abstract：:Functional atrophy and accompanying lymphocytic infiltration and destruction of the lacrimal gland (LG) are characteristics of Sjögren's Syndrome (SjS). The male NOD mouse is an experimental model for the autoimmune exocrinopathy that develops in the LG of SjS patients. Acinar cells in LG of male NOD mice aged 3-4 mon...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/j.exer.2009.03.020

    authors： Wu K,Joffre C,Li X,MacVeigh-Aloni M,Hom M,Hwang J,Ding C,Gregoire S,Bretillon L,Zhong JF,Hamm-Alvarez SF

    更新日期：2009-09-01 00:00:00

  • Role of aquaporin water channels in eye function.

    abstract：:The aquaporins (AQPs) are a family of more than 10 homologous water transporting proteins expressed in many mammalian epithelia and endothelia. At least five AQPs are expressed in the eye: AQP0 (MIP) in lens fiber, AQP1 in cornea endothelium, ciliary and lens epithelia and trabecular meshwork, AQP3 in conjunctiva, AQP...

    journal_title：Experimental eye research

    pub_type： 杂志文章,评审

    doi：10.1016/s0014-4835(02)00303-2

    authors： Verkman AS

    更新日期：2003-02-01 00:00:00

  • Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes.

    abstract：:Increasing evidence points to inflammation as a key factor in the pathogenesis of diabetic retinopathy (DR). Choroidal inflammatory changes in diabetes have been reported and in vivo choroidal thickness (CT) has been searched as a marker of retinopathy with contradictory results. We aimed to investigate the early stag...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/j.exer.2020.108195

    authors： Campos A,Campos EJ,Martins J,Rodrigues FSC,Silva R,Ambrósio AF

    更新日期：2020-10-01 00:00:00

  • Macromolecular markers in normal human retina and applications to human retinal disease.

    abstract：:Macromolecular cell markers are essential for the classification and characterization of the highly complex and cellularly diverse vertebrate retina. Although a plethora of markers are described in the current literature, the immunoreactivity of these markers in normal human tissue has not been fully determined. This ...

    journal_title：Experimental eye research

    pub_type： 杂志文章,评审

    doi：10.1016/j.exer.2016.01.002

    authors： de Souza CF,Nivison-Smith L,Christie DL,Polkinghorne P,McGhee C,Kalloniatis M,Acosta ML

    更新日期：2016-09-01 00:00:00

  • Pigment epithelium-derived factor inhibits erythropoietin-induced retinal endothelial cell angiogenesis by suppression of PI3K/Akt pathway.

    abstract：:Erythropoietin (EPO) plays a critical role in the vascular system and exhibits angiogenic activity in endothelial cells (ECs) such as stimulation of cell proliferation, migration and tube formation in vitro. EPO is the major regulator of cell proliferation and differentiation of erythroid precursors and there by preve...

    journal_title：Experimental eye research

    pub_type： 杂志文章,收录出版

    doi：10.1016/j.exer.2010.03.005

    authors： Haribalaganesh R,Sheikpranbabu S,Banumathi E,Gurunathan S

    更新日期：2010-06-01 00:00:00

  • Expression of inwardly rectifying potassium channel subunits in native human retinal pigment epithelium.

    abstract：:Previously, we demonstrated that the inwardly rectifying K(+) (Kir) channel subunit Kir7.1 is highly expressed in bovine and human retinal pigment epithelium (RPE). The purpose of this study was to determine whether any of the 14 other members of the Kir gene family are expressed in native human RPE. Conventional reve...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/j.exer.2008.05.010

    authors： Yang D,Zhang X,Hughes BA

    更新日期：2008-09-01 00:00:00

  • Identification of pathogenic factors potentially involved in Staphylococcus aureus keratitis using proteomics.

    abstract：:Staphylococcus is a leading cause of microbial keratitis, characterized by destruction of the cornea by bacterial exoproteins and host-associated factors. The aim of this study was to compare extracellular and cell-associated proteins produced by two different isolates of S. aureus, a virulent clinical isolate (Staph ...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/j.exer.2016.08.016

    authors： Khan S,Cole N,Hume EB,Garthwaite LL,Nguyen-Khuong T,Walsh BJ,Willcox MD

    更新日期：2016-10-01 00:00:00

  • Novel anti-angiogenic PEDF-derived small peptides mitigate choroidal neovascularization.

    abstract：:Abnormal migration and proliferation of endothelial cells (EC) drive neovascular retinopathies. While anti-VEGF treatment slows progression, pathology is often supported by decrease in intraocular pigment epithelium-derived factor (PEDF), an endogenous inhibitor of angiogenesis. A surface helical 34-mer peptide of PED...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1016/j.exer.2019.107798

    authors： Sheibani N,Wang S,Darjatmoko SR,Fisk DL,Shahi PK,Pattnaik BR,Sorenson CM,Bhowmick R,Volpert OV,Albert DM,Melgar-Asensio I,Henkin J

    更新日期：2019-11-01 00:00:00

  • Penetration of topical fluconazole into human aqueous humor.

    abstract：:The purpose of this study was to determine the aqueous levels and pharmacokinetics of topical fluconazole 0.2% upon single and multiple drop applications. Forty-nine patients undergoing cataract surgery were given topical fluconazole 0.2%. They either received single drop or a loading dose of 1 drop per 5 min for 20 m...

    journal_title：Experimental eye research

    pub_type： 杂志文章

    doi：10.1006/exer.2000.0936

    authors： Abbasoğlu OE,Hoşal BM,Sener B,Erdemoğlu N,Gürsel E

    更新日期：2001-02-01 00:00:00