Abstract:
:Erythropoietin (EPO) plays a critical role in the vascular system and exhibits angiogenic activity in endothelial cells (ECs) such as stimulation of cell proliferation, migration and tube formation in vitro. EPO is the major regulator of cell proliferation and differentiation of erythroid precursors and there by preventing the apoptosis. Pigment epithelial derived factor (PEDF) is a potent anti-angiogenic factor whose effects are partially mediated through the induction of EC apoptosis. The mechanism of EPO and PEDF in retinal neovascularization has not been well documented yet. The effect of EPO and PEDF on cell proliferation was determined by MTT assay. In vitro wound-scratch assay was performed to study the migration of ECs and in vitro tube formation was assessed by the on-gel assay system using gelatin. Inhibitor assay was carried out using LY294002, a phosphatidylinositol 3-kinase (PI3K) inhibitor. Further, PI3K/Akt activity was assessed by transient transfection assay using constitutively active (CA) and dominant negative (DN) Akt mutants. Dextran permeability assay was performed to determine the vascular permeability. We report that EPO stimulates EC proliferation, migration, tube formation and permeability whereas PEDF inhibits the EPO-induced ECs proliferation and permeability. Over expression of DN Akt blocked EPO stimulation of proliferation and permeability, while over expression of CA Akt rescues the inhibitory effect of PEDF on proliferation and permeability. These results demonstrate that PEDF may inhibit the EPO-induced proliferation and permeability via PI3K/Akt-dependent pathway.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Haribalaganesh R,Sheikpranbabu S,Banumathi E,Gurunathan Sdoi
10.1016/j.exer.2010.03.005subject
Has Abstractpub_date
2010-06-01 00:00:00pages
726-33issue
6eissn
0014-4835issn
1096-0007pii
S0014-4835(10)00080-1journal_volume
90pub_type
杂志文章,收录出版abstract::Aceclidine can dissociate accommodative and outflow facility responses in monkeys and humans. We sought to determine if different muscarinic receptor subtypes control outflow facility, accommodative and pupillary responses to aceclidine in the living rhesus monkey eye, as a possible basis for this separation. Each eye...
journal_title:Experimental eye research
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abstract::Certain mutations in optineurin (gene OPTN) are associated with primary open angle glaucoma. Optineurin is ubiquitously expressed but it shows high level of expression in certain cells and tissues including retinal ganglion cells. It interacts with many proteins, often acting as an adaptor to link two or more proteins...
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abstract::The following lectins: Con A, WGA, sWGA, PNA, RCA and UEA were used to study developmental changes in the expression of glycoconjugates during chicken lens morphogenesis. Con A, WGA, sWGA binding epitopes were observed in the lens placode and vesicle. Once the fiber mass was formed, the glycoconjugates were mainly fou...
journal_title:Experimental eye research
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abstract::The R49C mutation of alphaA-crystallin (alphaA-R49C) causes hereditary cataracts in humans; patients in a four-generation Caucasian family were found be heterozygous for this autosomal dominant mutation. We previously generated knock-in mouse models of this mutation and found that by 2 months of age, heterozygous muta...
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journal_title:Experimental eye research
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.11.011
更新日期:2008-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0560
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journal_title:Experimental eye research
pub_type: 杂志文章
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journal_title:Experimental eye research
pub_type: 杂志文章
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.10.007
更新日期:2007-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1096
更新日期:1993-07-01 00:00:00
abstract::Anthocyanins (ACs) are phenolic compounds that are distributed widely in fruits and vegetables. Although consumption of these compounds has been shown to improve visual function, the distribution of ACs in ocular tissue has not been examined in detail. The aim of this study was therefore to evaluate the ocular distrib...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.12.019
更新日期:2006-08-01 00:00:00
abstract::Retinal neovascularization (NV) due to retinal ischemia is one of the major causes of vision reduction in patients with different types of retinal diseases although anti-vascular endothelial growth factor (anti-VEGF) therapy can partially reduce the size of the retinal NV. We recently reported that periostin plays an ...
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pub_type: 杂志文章
doi:10.1016/j.exer.2016.10.012
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1999.0703
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journal_title:Experimental eye research
pub_type: 杂志文章
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abstract::Nitric oxide (NO) production by vascular endothelium is important in regulation of blood flow. Reduced production of NO can adversely affect blood flow and other vascular functions. We investigated the expression of three nitric oxide synthase (NOS) isoforms in retina and choroid of aged human eyes and eyes with AMD. ...
journal_title:Experimental eye research
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pub_type: 临床试验,杂志文章,随机对照试验
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abstract::A dark reaction is known to occur in retinal extracts of the gecko (Gekko gekko), in which the natural 11-cis-chromophore of the 521-pigment is apparently replaced by adding 9-cis-retinal to form the 9-cis-photopigment. With chloride-deficient extracts the reaction involves some 70% of the 521-pigment. Anions like nit...
journal_title:Experimental eye research
pub_type: 杂志文章
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2008.03.013
更新日期:2008-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0608
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abstract::Aldose reductase (AR) is implicated in some of the disabling complications of diabetes, including neuropathy, retinopathy and cataracts. Our studies are aimed at further clarifying the role of AR in diabetes and facilitating the design of new classes of potent, specific AR inhibitors by gaining an understanding of the...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(89)90048-1
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pub_type: 杂志文章
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.12.008
更新日期:2007-04-01 00:00:00
abstract::Periorbital adipose tissue expansion is a key pathological change in thyroid associated orbitopathy (TAO). Bone morphogenic protein 4 (BMP4) is instrumental in adipogenesis. We compared site-specific BMP4 expression and its effect on adipogenesis using donor-matched adipose tissue-derived stromal cells (ADSC) from TAO...
journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2019-04-01 00:00:00