Abstract:
:The purpose of this investigation is to characterize parvalbumin-immunoreactive (IR) neurons in the inner nuclear layer (INL) of zebrafish retina through immunocytochemistry, quantitative analysis, and confocal microscopy. In the INL, parvalbumin-IR neurons were located in the inner marginal portion of the INL. On the basis of dendritic stratification in the inner plexiform layer (IPL), at least two types of amacrine cells were IR for parvalbumin. The first one formed distinctive laminar tiers within s4 (PVs4) of the IPL, and the second within s5 (PVs5). The average number of PVs4 cells was 8263 cells per retina (n=3), and the mean density was 1671cells/mm(2). The average number of PVs5 cells was 1037 cells per retina (n=3), and the mean density was 210cells/mm(2). Quantitatively, 88.9% of anti-parvalbumin labeled neurons were PVs4 cells and 11.1% were PVs5 cells. Their density was highest in the midcentral region of the ventrotemporal retina and lowest in the periphery of the dorsonasal retina. The average regularity index of the PVs4 cell mosaic was 4.09, while the average regularity index of the PVs5 cell mosaic was 3.46. No parvalbumin-IR cells expressed calretinin or disabled-1, markers for AII amacrine cells, in several animals. These results indicate that parvalbumin-IR neurons in zebrafish are limited to specific subpopulations of amacrine cells and the expressional pattern of parvalbumin may not correspond to AII amacrine cells in several other animals. Their distribution suggests that parvalbumin-IR neurons are mainly involved in ON pathway information flow.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Yeo JY,Lee ES,Jeon CJdoi
10.1016/j.exer.2008.11.014subject
Has Abstractpub_date
2009-03-01 00:00:00pages
553-60issue
3eissn
0014-4835issn
1096-0007pii
S0014-4835(08)00399-0journal_volume
88pub_type
杂志文章abstract::Cell lines derived from the lens generally fail to maintain synthesis of crystallins in long-term culture. Here we demonstrate that the N/N1003A line of undifferentiated lens epithelial cells, derived from a newborn rabbit, does not produce detectable levels of alpha-, beta- or gamma-crystallin transcripts, yet is cap...
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journal_title:Experimental eye research
pub_type: 杂志文章
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pub_type: 杂志文章,评审
doi:10.1016/j.exer.2005.03.006
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journal_title:Experimental eye research
pub_type: 杂志文章
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pub_type: 杂志文章
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