Abstract:
:We have reported that the aspartyl (Asp)-151 residue in alphaA-crystallin in human eye lens was inverted to the D-isomer and isomerized to beta-Asp residue with age. We report here that ultraviolet (UV)-B irradiation induces the racemization and isomerization of the Asp-151 residue of alphaA-crystallin from lenses of 6-week-old rats to form D-isomer and beta-Asp residue. Simultaneous racemization and isomerization of the specific Asp residue indicate that the reaction proceeds via formation of a succinimide intermediate. This modification was not observed in UV-A irradiated and normal lenses. UV-B irradiation induced the racemization of only the Asp-151 residue and did not affect the other Asp residues in alphaA-crystallin. On the other hand, the high molecular weight fraction of the lens protein increased upon UV-B irradiation. Modification of the Asp residue would affect the three-dimensional packing array of the lens protein.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Fujii N,Momose Y,Ishibashi Y,Uemura T,Takita M,Takehana Mdoi
10.1006/exer.1997.0315subject
Has Abstractpub_date
1997-07-01 00:00:00pages
99-104issue
1eissn
0014-4835issn
1096-0007pii
S0014-4835(97)90315-8journal_volume
65pub_type
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