Abstract:
:A number of different agents, such as growth factors, cytokines and phorbol esters have been shown to modulate trabecular meshwork cell function. These studies were designed to evaluate the role extracellular signal-regulated kinase (ERK) pathway plays in mediating the responses to platelet-derived growth factor-BB (PDGF-BB) and phorbol 12-myristate 13-acetate (PMA) in trabecular meshwork cells. The human trabecular meshwork cell line, HTM-3, and the bovine trabecular meshwork (BTM) cells were treated with either PDGF-BB or PMA and the activation of ERK 1/2 evaluated. The effects of the MAP kinase kinase (MEK) inhibitor U0126, and the PKC inhibitor chelerythrine on ERK 1/2 were also determined. In a separate group of experiments, cells were treated with PDGF-BB or PMA and the secretion of matrix metalloproteinase-2 (MMP-2) evaluated. The addition of PDGF-BB or PMA produced time- and dose-dependent activation of ERK 1/2. Pretreatment with U0126 or chelerythrine significantly reduced ERK 1/2 activation induced by PDGF-BB or PMA. The addition of PDGF-BB or PMA stimulated the secretion of MMP-2. This secretory response was inhibited by pretreatment with the MEK inhibitor U0126. In trabecular meshwork cells, PDGF-BB and PMA activate ERK 1/2 by a PKC-dependent mechanism. Activation of ERK 1/2 by these agents in trabecular meshwork cells leads to the secretion of MMP-2. These studies provide evidence that ERK pathway is an important mechanism for integrating various signals that regulate trabecular function.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Shearer T,Crosson CEdoi
10.1006/exer.2001.1007keywords:
subject
Has Abstractpub_date
2001-07-01 00:00:00pages
25-35issue
1eissn
0014-4835issn
1096-0007pii
S0014-4835(01)91007-3journal_volume
73pub_type
杂志文章abstract::Cultured bovine lens epithelial cells adapt to hypertonic sodium stress via an increase in Na(+)-myo-inositol cotransporter activity and accumulate myo-inositol. At least 12 hr of hypertonic exposure was necessary to enhance myo-inositol accumulation; and thereafter, uptake activity continued to increase throughout th...
journal_title:Experimental eye research
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journal_title:Experimental eye research
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更新日期:1984-07-01 00:00:00
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journal_title:Experimental eye research
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0194
更新日期:1997-02-01 00:00:00
abstract::We have examined the possible expression of fibroblast growth factor in cultured cells derived from bovine lens epithelium and corneal endothelium. Lens epithelial, but not corneal endothelial, cells were found to express the acidic fibroblast growth factor (aFGF) gene, whereas both cell types express the gene encodin...
journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:1988-01-01 00:00:00
abstract::Oxidative stress and inflammation are important pathological mechanisms in many neurodegenerative diseases, including age-related macular degeneration (AMD). The very low-density lipoprotein receptor knockout mouse (Vldlr-/-) has been identified as a model for AMD and in particular for retinal angiomatous proliferatio...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.08.003
更新日期:2013-11-01 00:00:00
abstract::Glutamate is an excitatory neurotransmitter in the central and peripheral nervous systems of the vertebrate. The previous studies show the presence of mRNAs of AMPA-type glutamate receptors, GluR1 and GluR2, in the optic tectum of the pigeon, suggesting glutamatergic input from the retina. The present study examined l...
journal_title:Experimental eye research
pub_type: 信件
doi:10.1016/j.exer.2009.03.024
更新日期:2009-09-01 00:00:00
abstract::Sodium hyaluronates with molecular weights of 18000, 500000 and 4 x 10(6), labelled with either 3H or 14C, were injected into the anterior chamber or into the centre of the vitreous body of rabbits and their rates of disappearance were followed. The out-flow from the anterior chamber in anaesthetized animals was virtu...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(83)90043-x
更新日期:1983-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.09.015
更新日期:2016-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(92)90076-5
更新日期:1992-01-01 00:00:00
abstract::Mitochondrial damage and epigenetic modifications have been implicated in the pathogenesis of Age-related Macular Degeneration (AMD). This study was designed to investigate the effects of AMD/normal mitochondria on epigenetic regulation in human transmitochondrial retinal pigment epithelial (RPE) cells in vitro. Human...
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doi:10.1016/j.exer.2019.107701
更新日期:2019-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0286
更新日期:1997-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:1989-05-01 00:00:00
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2015.01.018
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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更新日期:2006-04-01 00:00:00
abstract::We have used a polyclonal antiserum to bovine ciliary epithelium, a secretory tissue involved in the formation of aqueous humor, to immunoscreen a directional lambda gt11 Sfi-Not cDNA expression library prepared from bovine ciliary epithelium poly(A)+ RNA. After immunoscreening 6 x 10(5) independent clones, 41 cDNA cl...
journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:1992-11-01 00:00:00
abstract::With the combined purpose of facilitating useful vision over a lifetime, a number of ocular cells have evolved specialized features not found elsewhere in the body. The trabecular meshwork (TM) cell at the irido-corneal angle, which is a key regulator of intraocular pressure, is no exception. Examination of cells in c...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
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更新日期:2017-05-01 00:00:00
abstract::SPARC (secreted protein acidic and rich in cysteine) is a matricellular protein that regulates cellular adhesion and proliferation. In this report, we show that SPARC protein is restricted to epithelial cells of the murine lens and ends abruptly at the equatorial bow region where lens fiber differentiation begins. SPA...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0608
更新日期:1999-03-01 00:00:00
abstract::This study evaluated the efficacy of Plasma rich in growth factors (PRGF-Endoret) on the corneal wound healing process after Photorefractive keratectomy (PRK). To address this, blood from three healthy donors was collected, centrifuged and, the whole plasma column (WP) and the plasma fraction with the highest platelet...
journal_title:Experimental eye research
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更新日期:2013-10-01 00:00:00
abstract::The purpose of this study was to determine the aqueous levels and pharmacokinetics of topical fluconazole 0.2% upon single and multiple drop applications. Forty-nine patients undergoing cataract surgery were given topical fluconazole 0.2%. They either received single drop or a loading dose of 1 drop per 5 min for 20 m...
journal_title:Experimental eye research
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abstract::Choroidal neovascularization (CNV) is one of the most frequent causes of severe and progressive vision loss, while its pathogenesis is still poorly understood. Focal adhesion kinase (FAK), a non-receptor tyrosine kinase, plays a crucial role in linking signals initiated by both the extracellular matrix (ECM) and solub...
journal_title:Experimental eye research
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doi:10.1016/j.exer.2008.11.034
更新日期:2009-05-01 00:00:00
abstract::When 15-day-old developing chick embryos were administered hydrocortisone hemisuccinate sodium (HC:0.25 mumol per egg) the concentration of glucose in both the aqueous humor and the vitreous body began to increase significantly after 3 hr and reached 24.4 +/- 0.3 mM and 22.3 +/- 0.9 mM, respectively, at 48 hr. Thereaf...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(89)80050-8
更新日期:1989-10-01 00:00:00
abstract::Research over the past two decades has shown that the growth of young eyes is guided by vision. If near- or far-sightedness is artificially imposed by spectacle lenses, eyes of primates and chicks compensate by changing their rate of elongation, thereby growing back to the pre-lens optical condition. Little is known a...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1999.0813
更新日期:2000-04-01 00:00:00