Abstract：
:There has been a drive to develop new cell based therapies to treat corneal blindness, one of the most common causes of blindness worldwide. Mechanical and physical cues are known to regulate the behavior of many cell types, however studies examining these effects on corneal epithelial cells have been limited in number and their findings have not previously been amalgamated and contrasted. Here, we provide an overview of the different types of mechanical stimuli to which the corneal epithelium is exposed and the influence that these have on the cells. Shear stress from the tear film motion and blinking, extracellular matrix stiffness and external physical forces such as eye rubbing and contact lens wear are among some of the forms of mechanical stimuli that the epithelium experiences. In vivo and in vitro studies examining the mechanobiology on corneal epithelial cells under differing mechanical environments are explored. A greater understanding of the mechanobiology of the corneal epithelium has the potential to lead to improved tissue engineering and cell based therapies to repair and regenerate damaged cornea.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Masterton S,Ahearne Mdoi
10.1016/j.exer.2018.08.001subject
Has Abstractpub_date
2018-12-01 00:00:00pages
122-129eissn
0014-4835issn
1096-0007pii
S0014-4835(18)30208-2journal_volume
177pub_type
杂志文章,评审abstract：:Utilizing a human beta-actin promoter, a catalase cDNA expression vector was constructed. This construct was used to transfect two immortal cell lines, mouse alpha TN4-1 and rabbit N/N 1003A. The catalase activity was increased about 3.4 fold in the alpha TN4-1 cells and 38 fold in the N/N 1003A cells. Some changes in...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0560
更新日期：1998-12-01 00:00:00
abstract：:Two previous studies have shown that N(G)-nitro-L-arginine methyl ester (L-NAME), an inhibitor of neuronal nitric oxide synthase, protects retinas of albino rats and mice from damaging levels of light. The aims of the present study were two-fold: (1) to confirm the protective effect of L-NAME on wild type albino rats ...
journal_title：Experimental eye research
pub_type： 杂志文章
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abstract：:The purpose of this study was to quantify how the elastic modulus of the ex vivo iris changes following stimulation by pilocarpine (PILO), phenylephrine (PE), and tropicamide (TROP). Irides (n = 20) were dissected from porcine eyes within 4 h post-mortem and tested uniaxially. Either the entire iris or sector thereof ...
journal_title：Experimental eye research
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doi：10.1016/j.exer.2009.04.014
更新日期：2009-10-01 00:00:00
abstract：:Transforming growth factor-beta (TGF-β) is one of the main epithelial-mesenchymal transition (EMT)-inducing factors. In general, TGF-β-induced EMT promotes cell migration and invasion. TGF-β also acts as a potent regulator of pericellular proteolysis by regulating the expression and secretion of plasminogen activators...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.06.020
更新日期：2013-10-01 00:00:00
abstract：:In order to develop effective optical treatment strategies for myopia, it is important to understand how visual experience influences refractive development. Beginning with the discovery of the phenomenon of form deprivation myopia, research involving many animal species has demonstrated that refractive development is...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2012.11.019
更新日期：2013-09-01 00:00:00
abstract：:In the neural retina, glial cells control formation of ionic gradients by mediating transmembrane water fluxes through aquaporin (AQP) water channels. Retinal content and immunolocalization of two water channels, AQP1 and AQP4, in the diabetic rat retinas during high-salt loading were examined in this study. Diabetes ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.06.020
更新日期：2009-11-01 00:00:00
abstract：:Retinoblastoma generally occurs before 5 years of age and often requires enucleation (surgical removal of one eye) of the cancerous eye. We have previously shown using behavioural methods that this disruption in binocular vision during the critical period of visual development results in impaired face perception. In t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.09.013
更新日期：2019-06-01 00:00:00
abstract：:Purified retinal pigment epithelial cells of bovine eyes have been fractionated by a series of buffer and detergent extractions. The electropherogram of the buffer-insoluble, Triton X-100-soluble fraction (RPE-TS) exhibits a major polypeptide band of M(r) 65 kDa and a variety of minor components. Electrophoretically p...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90008-g
更新日期：1992-12-01 00:00:00
abstract：:A potential role for myosin light chain kinase (MLCK) in regulating intraocular pressure and outflow function has recently been reported in living monkey eye and rabbit eye. There is little information about the effects of the ocular hypotensive agents, prostaglandin F2alpha (PGF2alpha) and latanoprost on this signali...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2003.10.015
更新日期：2004-02-01 00:00:00
abstract：:Lack of an optimal in vitro model of human corneal epithelial (HCE) cells is a major limitation in studying normal functions and gene regulations in HCE. Moreover, availability of a multi-layered HCE culture can reduce the usage of animals in the toxicity testing of consumer products. We have developed tetracycline-re...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(03)00175-1
更新日期：2003-10-01 00:00:00
abstract：:Myo/Nog cells are essential for eye development in the chick embryo and respond to injury in adult tissues. These cells express mRNA for the skeletal muscle specific transcription factor MyoD, the bone morphogenetic protein (BMP) inhibitor Noggin and the cell surface protein recognized by the G8 monoclonal antibody (m...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2015.11.023
更新日期：2016-05-01 00:00:00
abstract：:Corneal autofluorescence, as measured with a commercial scanning fluorophotometer (lambda(exc): 415-491 nm; lambda(em): 515-630 nm), is increased in patients with diabetes mellitus. However, such fluorophotometers register an average fluorescence signal over all corneal layers as a consequence of their limited axial r...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0733
更新日期：1999-11-01 00:00:00
abstract：:The purpose of this study was to evaluate the composition of ghost vessels and the newly identified intravitreal structures type 1 and 2 (IVS-1 and 2) observed in the rabbit vitreous body. Rabbit eyes (n = 10, 0.5- approximately 36 months of age) were fixed and embedded in plastic. Post-embedding immuno transmission e...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0865
更新日期：2000-08-01 00:00:00
abstract：:Recently we determined that the Transient Receptor Potential Vanilloid 4 ion channel (TRPV4) has a crucial signaling role in a pathway that regulates various aspects of lens epithelium function. Here, we report on a different TRPV channel, TRPV1, in porcine lens. The presence of TRPV1 in the lens was evident from RT-P...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.04.006
更新日期：2018-07-01 00:00:00
abstract：:This study tests the hypothesis that choroidal myogenic autoregulation participates in the intraocular pressure (IOP) response to mean arterial pressure (MAP) by minimizing arterial-pressure-dependent changes in choroidal blood volume. To test this hypothesis, the IOP response to MAP was quantified before and after im...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1047
更新日期：1994-05-01 00:00:00
abstract：:Post-translational modifications by transglutaminase may contribute to the remodeling of cellular architecture in the development of lens fiber cells, and there is evidence that the enzyme may also play a role in cataract formation. It catalyses hydrolytic deamidations as well as amide exchanges on select glutamine si...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0463
更新日期：1998-05-01 00:00:00
abstract：:Angiotensin II and aldosterone are the main effectors of the renin-angiotensin aldosterone system (RAAS) and have a central role in hypertension as well as cardiovascular and renal disease. The localization of RAAS components within the retina has led to studies investigating the roles of angiotensin II, aldosterone a...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2019.107766
更新日期：2019-10-01 00:00:00
abstract：:Fibroblast growth factors play a key role in regulating lens epithelial cell proliferation and differentiation via an anteroposterior gradient that exists between the aqueous and vitreous humours. FGF-2 is the most important for lens epithelial cell proliferation and differentiation. It has been proposed that the pres...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.12.004
更新日期：2014-03-01 00:00:00
abstract：:Recent studies have shown that lactate coupled water flux is the underlying mechanism of the corneal endothelial pump, which is highly dependent on the presence of bicarbonate. In this study we test the hypothesis that the increased intracellular pH (pHi) caused by bicarbonate stimulates glycolytic activity and the pr...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108193
更新日期：2020-10-01 00:00:00
abstract：:Forskolin, a diterpene, a potent and direct stimulator of the catalytic unit of adenylate cyclase, can reduce intraocular pressure when administered topically to rabbit eyes in a 1% suspension. Using an intravitreal plant of fluorescein-dextran, aqueous flow was measured by a modification of the method of Maurice. For...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90113-1
更新日期：1984-07-01 00:00:00
abstract：:The response of the lens to changes in the distribution and concentration of electrolytes can be measured by characterizing the changes in resistivity that occur secondary to variations in intracellular composition. Regional intracellular cytoplasmic resistivities of lens fiber cells can be approximated by homogenates...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80164-1
更新日期：1987-04-01 00:00:00
abstract：:Familial exudative vitreoretinopathy (FEVR) is a disease exhibits a wide range of clinical signs, ranging mild peripheral retinal vascular anomalies to severe retinal detachments. Individuals with mild FEVR are frequently asymptomatic with good visual function and are often undiagnosed. However, little is known about ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.107941
更新日期：2020-03-01 00:00:00
abstract：:Eyes with age-related macular degeneration (AMD) demonstrate accumulation of specific deposits and extracellular matrix (ECM) molecules under the retinal pigment epithelium (RPE). AMD is about two times more prevalent in aging postmenopausal women. Therefore we studied whether 17beta-estradiol (E(2)) modulates the exp...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.01.010
更新日期：2008-04-01 00:00:00
abstract：:The levels of alpha 1-proteinase inhibitor (alpha 1-antitrypsin) in keratoconus, normal human, and other diseased corneas were examined. Using an immunoperoxidase technique, the presence of this inhibitor was demonstrated in the epithelium, stroma and endothelium of all corneal sections. Compared with normal human con...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90044-u
更新日期：1990-05-01 00:00:00
abstract：:Retinitis Pigmentosa (RP) is an inherited disorder that may lead to blindness. In the rhodopsin S334ter-line-3 rat model of RP, the death of rods induces spatial rearrangement of cones into regular ring mosaics. Using this model, we discovered that the ring mosaics are restored to a homogeneous distribution upon appli...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.08.005
更新日期：2015-11-01 00:00:00
abstract：:When 15-day-old developing chick embryos were administered hydrocortisone hemisuccinate sodium (HC:0.25 mumol per egg) the concentration of glucose in both the aqueous humor and the vitreous body began to increase significantly after 3 hr and reached 24.4 +/- 0.3 mM and 22.3 +/- 0.9 mM, respectively, at 48 hr. Thereaf...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(89)80050-8
更新日期：1989-10-01 00:00:00
abstract：:In ocular surface inflammatory diseases, such as dry eye disease, long-term symptom relief requires targeting the inflammation itself rather than treating only the surface-associated dryness with artificial tears. Therefore, we included an anti-inflammatory agent in an unpreserved liposome-based (LP) formulation used ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.11.010
更新日期：2017-01-01 00:00:00
abstract：:We studied a new fluorophotometric method to determine trabecular outflow facility in man. Pilocarpine was selected to evaluate this non-invasive method, since its primary ocular hypotensive effect is to increase trabecular outflow facility. Levobunolol was used to decrease intraocular pressure and aqueous humor flow ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(89)90004-3
更新日期：1989-05-01 00:00:00
abstract：:The facilitated diffusion glucose transporter in the plasma membrane of intact outer segments isolated from bovine retinal rods (ROS) was characterized by measurements of: (1) 14C-labeled 3-O-methylglucose fluxes; and (2) glucose-sensitive binding of 3H-labeled cytochalasin B to ROS membranes. Inhibition of 3-O-methyl...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1117
更新日期：1994-09-01 00:00:00
abstract：PURPOSE:Cellular therapy with mesenchymal stem cells (MSC) is emerging as an effective option to treat optic neuropathies. In models of retinal degeneration, MSC injected in the vitreous body protects injured retinal ganglion cells and stimulate their regeneration, however the mechanism is still unknown. Considering th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108212
更新日期：2020-11-01 00:00:00
