Abstract:
PURPOSE:Cellular therapy with mesenchymal stem cells (MSC) is emerging as an effective option to treat optic neuropathies. In models of retinal degeneration, MSC injected in the vitreous body protects injured retinal ganglion cells and stimulate their regeneration, however the mechanism is still unknown. Considering the immunomodulating proprieties of MSC and the controversial role of microglial contribution on retinal regeneration, we developed an in vitro co-culture model to analyze the effect of MSC on retinal microglia population. METHODS:We used whole adult rat retinal explants in co-culture with human Wharton's jelly mesenchymal stem cells (hMSC) separated by a transwell membrane and analyzed hMSC effect on both retinal ganglion cells (RGCs) and retinal microglia. RESULTS:hMSC in co-culture protected RGCs after 3 days in vitro by paracrine signaling. In addition, hMSC reduced microglia population and inhibited the pro-inflammatory phenotype of the remaining microglia. CONCLUSIONS:Using a co-culture model, we demonstrated the paracrine effect of hMSC on RGC survival after injury concomitant with a reduction of microglial population. Paracrine signaling of hMSC also changed microglia phenotype and the expression of antiinflammatory factors in the retina. Our results are consistent with a detrimental effect of microglia on RGC survival and regeneration after injury.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Teixeira-Pinheiro LC,Toledo MF,Nascimento-Dos-Santos G,Mendez-Otero R,Mesentier-Louro LA,Santiago MFdoi
10.1016/j.exer.2020.108212subject
Has Abstractpub_date
2020-11-01 00:00:00pages
108212eissn
0014-4835issn
1096-0007pii
S0014-4835(20)30470-Xjournal_volume
200pub_type
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