Abstract：
:The facilitated diffusion glucose transporter in the plasma membrane of intact outer segments isolated from bovine retinal rods (ROS) was characterized by measurements of: (1) 14C-labeled 3-O-methylglucose fluxes; and (2) glucose-sensitive binding of 3H-labeled cytochalasin B to ROS membranes. Inhibition of 3-O-methylglucose influx into ROS, inhibition of 3-O-methylglucose efflux from ROS and glucose-sensitive binding of cytochalasin B to ROS showed very similar cytochalasin B inhibition/dissociation constants of 0.9 microM, 1.3 microM and 1.3 microM, respectively. D-glucose inhibited both 14C-labeled 3-O-methylglucose transport and cytochalasin B binding. The above results suggest that D-glucose-sensitive cytochalasin B binding reflects specific binding to the ROS glucose transporter and the density of glucose transporter in the ROS plasma membrane was determined to be 800 microns-2, comparable to relatively abundant ROS plasma membrane proteins such as the cGMP-gated channel and the Na-Ca+K exchanger. Displacement of 3H-labeled cytochalasin B by non-transportable hexoses was used to localize the hexose transporters to the ROS plasma membrane and to examine a simple single-site, alternating conformation model for hexose transport. A comparison between the Glut1 glucose transporters of bovine ROS, bovine erythrocytes and human erythrocytes suggests that kinetic and pharmacological characteristics of glucose transporters cannot be predicted in a simple manner from gene type and species.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Li XB,Szerencsei RT,Schnetkamp PPdoi
10.1006/exer.1994.1117subject
Has Abstractpub_date
1994-09-01 00:00:00pages
351-8issue
3eissn
0014-4835issn
1096-0007pii
S0014-4835(84)71117-1journal_volume
59pub_type
杂志文章abstract：:Matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF) are all implicated in the development of neovascularization. To investigate the possible role of these factors in corneal neovascularization we have analysed the expression of MMP-2, MMP-9 and VEGF in a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0790
更新日期：2000-04-01 00:00:00
abstract：:Docosahexaenoic acid is the major polyunsaturated fatty acid in photoreceptor membrane phospholipids and is thought to be necessary for photoreceptor function. Docosahexaenoic acid may be metabolized to docosanoids or, by retroconversion, to eicosapentaenoic acid followed by lipoxygenation and synthesis of n-3 metabol...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0116
更新日期：1996-09-01 00:00:00
abstract：:We have used a polyclonal antiserum to bovine ciliary epithelium, a secretory tissue involved in the formation of aqueous humor, to immunoscreen a directional lambda gt11 Sfi-Not cDNA expression library prepared from bovine ciliary epithelium poly(A)+ RNA. After immunoscreening 6 x 10(5) independent clones, 41 cDNA cl...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90175-r
更新日期：1992-11-01 00:00:00
abstract：:The advance of optical coherence tomography (OCT) enables a detailed examination of the human retina in-vivo for clinical routine and experimental eye research. Only few investigations to date captured human foveal morphology in a large subject group on the basis of a detailed analysis employing mathematical models. H...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.05.013
更新日期：2016-07-01 00:00:00
abstract：:The presence of the alpha(1)-adrenoceptor subtypes in various parts of the pig and rabbit eyes was investigated using [(3)H]-prazosin radioligand binding. The characterization of the subtypes was achieved by performing competition experiments with various subtype selective drugs. In the pig retina, both alpha(1A)- and...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0753
更新日期：2000-01-01 00:00:00
abstract：:Mutations in the optic atrophy type 1 (OPA1) gene give rise to human autosomal dominant optic atrophy. The purpose of this study is to investigate OPA1 protein expression in the human retina and optic nerve. A rabbit polyclonal antiserum was generated using a fusion protein covering amino acids 647 to 808 of the human...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.06.004
更新日期：2006-11-01 00:00:00
abstract：:A key model for examining the activity-dependent development of primary visual cortex (V1) involves the imbalance in activity between the two eyes induced by monocular deprivation (MD). MD early in life causes dramatic changes in the functional and structural organization of mammalian visual cortex. The molecular sign...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.12.016
更新日期：2013-04-01 00:00:00
abstract：:Long living animal models of retinitis pigmentosa (RP) can provide important information on the retinal changes that occur at the late stages of photoreceptor degeneration. The rhodopsin Pro347Leu transgenic rabbit (P347L Tg) is a model of RP, and it has been used to analyze the functional and morphological changes in...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.04.026
更新日期：2019-07-01 00:00:00
abstract：:Tissue transglutaminase (tTG) is a Ca(2+)-dependent enzyme catalyzing the formation of covalent crosslinks between peptide-bound glutamine and lysine residues. Lens crystallins, including alphaB-crystallin and several beta-crystallins, are in vitro substrates for tTG. In both human and bovine fetal lens extracts treat...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.11.011
更新日期：2008-02-01 00:00:00
abstract：:Intraocular pressure (IOP) is the most critical risk factor for primary open angle glaucoma (POAG). In most cases of POAG, IOP is increased because of an abnormally high aqueous humor outflow resistance in the juxtacanalicular region of the trabecular meshwork. A distinct structural change in the trabecular meshwork o...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2009.01.005
更新日期：2009-04-01 00:00:00
abstract：:The purpose of this study was to investigate the effects of soluble VEGF on human choroidal endothelial cell (CEC) transmigration across an RPE monolayer as it relates to choroidal neovascularization in AMD. In coculture assays, ARPE-19 (ARPE) was plated on the undersides of Transwell inserts having 0.4 microm pores. ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.08.021
更新日期：2006-04-01 00:00:00
abstract：:The distribution of chondroitin sulfate proteoglycans in the mouse interphotoreceptor matrix (IPM) proximal to the retinal pigment epithelium (RPE) was evaluated with EM histochemical techniques using Cupromeronic Blue (CmB) before and after retinal separation. Densely packed, sheet-like processes surrounding vesicle-...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90054-v
更新日期：1992-03-01 00:00:00
abstract：:The polarity of retinal pigmented epithelia (RPE) from chicken embryos was studied in primary cell culture. Since cultured RPE approximates the morphological polarity of RPE in vivo, we investigated whether this polarity extends to the distribution of plasma membrane proteins that are peculiar to RPE. In contrast to o...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90156-o
更新日期：1990-10-01 00:00:00
abstract：:The biomechanical environment within the eye is of interest in both the regulation of intraocular pressure and the loss of retinal ganglion cell axons in glaucomatous optic neuropathy. Unfortunately, this environment is complex and difficult to determine. Here we provide a brief introduction to basic concepts of mecha...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2015.05.024
更新日期：2015-12-01 00:00:00
abstract：:Due to the high toxicity of aminoglycoside antibiotics, many authors prefer to use third generation cephalosporines in the prophylaxis and treatment of intraocular infections. The aim of the present study was to determine safe ceftazidime levels in anterior chamber irrigation solution. Twenty-two eyes of 12 white New ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0167
更新日期：1996-12-01 00:00:00
abstract：:An enzymatic procedure for the estimation of organic hydroperoxides has been adapted to biological tissues and applied to the measurement of hydroperoxides in the rat retina. Hydroperoxides are determined from the coupled activities of glutathione peroxidase and glutathione reductase as measured by the loss of NADPH a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90116-1
更新日期：1983-03-01 00:00:00
abstract：:The study aimed to determine the variation in thickness and biomechanical properties between the different regions of the human sclera. Thickness measurements were carried out along eight meridian lines extending from the posterior pole to limbus in 36 human donor scleras aged 52-96 years. Strip specimens were extract...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.02.010
更新日期：2010-05-01 00:00:00
abstract：:Human tear fluid was collected with blunted glass capillaries before and after performing the Schirmer test (ST). The test caused an increase in the tear fluid (TF) plasmin concentration (from 0.40 +/- 0.29 microgram ml-1 (mean +/- S.E.M.) to 1.14 +/- 0.26 microgram ml-1; Mann-Whitney test P less than 0.001) and a dec...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90223-h
更新日期：1990-02-01 00:00:00
abstract：:TGFβ2 induces extracellular matrix (ECM) remodeling and alters the cytoskeleton by both the canonical Smad and non-canonical signaling pathways. TGFβ2 regulates the expression of ECM proteins in trabecular meshwork (TM) cells, increases intraocular pressure (IOP) in an ex vivo perfusion organ culture model, and induce...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2013.10.017
更新日期：2013-11-01 00:00:00
abstract：:Eleven monoclonal antibodies against different segments of bovine rhodopsin were used with immunogold-dextran markers to label Lowicryl thin sections of bovine and frog retinal photoreceptor cells for visualization by transmission electron microscopy. Antibodies against the C-terminus, F1-F2 loop and N-terminus of rho...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(86)90017-5
更新日期：1986-01-01 00:00:00
abstract：:This study of lens protein composition found that some cytoskeletal proteins were degraded during the earliest stages of cataract formation. Cataract was induced in 13-14 day old rats by a single subcutaneous injection of sodium selenite (19 mumol kg-1). By 24 hr after the injection of selenite, the ratio of insoluble...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0220
更新日期：1997-03-01 00:00:00
abstract：:Utilizing cultured lenses from normal and homozygous glutathione peroxidase (GSHPx-1) knockout mice and inhibitors for GSSG Reductase (GSSG Red), 1,3-bis(2-chlorethyl)-1-nitrosourea (BCNU) and catalase (Cat), 3-aminotriazole (3-AT), the ability to degrade H2O2 was examined at two H2O2 concentrations, 300 microM and 80...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0250
更新日期：1997-03-01 00:00:00
abstract：:The nature of glucose transport at the microvascular blood-retinal barrier was studied using primary cultures of microvascular endothelial cells from bovine retina. Uptake of 3-O-methyl-D-glucose (3MG), a non-metabolizable glucose analogue, was rapid and equilibrative. 3MG uptake could be inhibited by traditional gluc...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90011-8
更新日期：1983-02-01 00:00:00
abstract：:It has been suggested that human iris pigment epithelial (IPE) cells isolated from iridectomized tissue could be used as autologous cells for transplantation into the subretinal space in diseases with dysfunctional retinal pigment epithelium (RPE). RPE cells synthesize a number of cytokines and their receptors which a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0517
更新日期：1998-08-01 00:00:00
abstract：:Retinal ischemia/reperfusion (IR) injury causes profound tissue damage, especially retinal ganglion cell death. The aims of the study were twofold: (1) to investigate the benefits of epigallocatechin-3-gallate (EGCG), the major catechin found in tea, after IR challenge, and (2) to elucidate the mechanism of EGCG inhib...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.01.008
更新日期：2008-04-01 00:00:00
abstract：:Periorbital adipose tissue expansion is a key pathological change in thyroid associated orbitopathy (TAO). Bone morphogenic protein 4 (BMP4) is instrumental in adipogenesis. We compared site-specific BMP4 expression and its effect on adipogenesis using donor-matched adipose tissue-derived stromal cells (ADSC) from TAO...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.01.020
更新日期：2019-04-01 00:00:00
abstract：:Glycoconjugate mucin secretion from conjunctival goblet cells is tightly regulated by nerves and specialized pro-resolving mediators (SPMs) to maintain ocular surface health. Here we investigated the actions of the SPM resolvin E1 (RvE1) on cultured rat conjunctival goblet cell glycoconjugate secretion and intracellul...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.04.015
更新日期：2018-08-01 00:00:00
abstract：:Multidrug resistance associated protein (MRP) is a major family of efflux transporters involved in drug efflux leading to drug resistance. The objective of this study was to explore physical barriers for ocular drug absorption and to verify if the role of efflux transporters. MRP-2 is a major homologue of MRP family a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.08.015
更新日期：2007-01-01 00:00:00
abstract：:Homocysteine is an amino acid required for the metabolism of methionine. Excess homocysteine is implicated in cardiovascular and neurological disease and new data suggest a role in various retinopathies. Mice lacking cystathionine-beta-synthase (cbs(-/-)) have an excess of retinal homocysteine and develop anatomical a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.12.011
更新日期：2012-03-01 00:00:00
abstract：:Arf encodes an important tumor suppressor, p19(Arf), which also plays a critical role to control hyperplasia in the primary vitreous during mouse eye development. In the absence of Arf, mice are born blind and display a phenotype closely mimicking severe forms of the human eye disease, persistent hyperplastic primary ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.01.004
更新日期：2016-04-01 00:00:00
