Abstract：
:Human tear fluid was collected with blunted glass capillaries before and after performing the Schirmer test (ST). The test caused an increase in the tear fluid (TF) plasmin concentration (from 0.40 +/- 0.29 microgram ml-1 (mean +/- S.E.M.) to 1.14 +/- 0.26 microgram ml-1; Mann-Whitney test P less than 0.001) and a decrease in plasminogen activator activity (from 2.04 +/- 0.24 IU ml-1 to 1.04 +/- 0.17 IU ml-1; Mann-Whitney test P less than 0.002). In the filter paper used for ST, that segment that had been in contact with the conjunctiva (5 mm) usually contained higher amounts of plasmin than the following 5 mm of the paper overhanging the lidmargin. The present results indicate that the TF collection may affect the plasminogen activator-plasmin system in TF, depending on the collection method used.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
van Setten GB,Stephens R,Tervo T,Salonen EM,Tarkkanen A,Vaheri Adoi
10.1016/0014-4835(90)90223-hsubject
Has Abstractpub_date
1990-02-01 00:00:00pages
135-41issue
2eissn
0014-4835issn
1096-0007pii
0014-4835(90)90223-Hjournal_volume
50pub_type
杂志文章abstract：:This is a brief overview of multifaceted anatomical, experimental and clinical studies conducted by the author since 1955 on the optic nerve head circulation in health and disease. Conclusions, based on the accumulated information provided by these studies, are summarized. The studies on the pattern of blood supply of...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/s0014-4835(05)80121-6
更新日期：1995-09-01 00:00:00
abstract：:We have investigated: (a) Phospholipid composition and phosphoinositide-PO4 turnover in rabbit cornea tissues; and (b) the effects of adrenergic and serotonergic agonists on breakdown of phosphoinositides in the rabbit cornea. The data obtained from these studies can be summarized as follows: (1) in the cornea phospha...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80047-7
更新日期：1987-06-01 00:00:00
abstract：:A perifusion system has been designed for metabolic nuclear magnetic resonance (NMR) spectroscopy studies of the bovine retina. This system, which allows the use of unmodified commercial probes for multinuclear studies, is shown here to maintain the perifused retina in a metabolic steady state at 25 degrees C, as dedu...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1175
更新日期：1993-12-01 00:00:00
abstract：:Mitochondrial components are of great importance for the maintenance of lens transparency. In our previous work, we confirmed that carbon monoxide (CO) can protect human and rabbit lens epithelial cells (LECs) from hydrogen peroxide (H2O2)-mediated apoptosis, while the mechanism remains undefined. Because CO can bind ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.01.023
更新日期：2018-04-01 00:00:00
abstract：:We designed an experiment to study the role of light history and polyunsaturated fatty acids (PUFA) on the susceptibility of the albino rat retina to light damage. Albino rats were born to dams that had been kept in either 1 lx or 250 lx cyclic light for 0-4 days prior to delivery and fed one of three diets containing...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80112-5
更新日期：1995-03-01 00:00:00
abstract：:A duplex fluorescence assay to assess the viability of cells cultured in multi-well plates is described, which can be carried out in the original culture plate using a plate reader, without exchanges of culture or assay medium, or transfer of cells or cell supernatant. The method uses freshly prepared reagents and doe...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.05.011
更新日期：2017-08-01 00:00:00
abstract：:Obesity has been associated with abnormal lipid metabolism and with tissue hypoxia. Human Bruch's membrane (BrM) lipid deposits have been proposed to create a diffusion barrier to metabolic exchange between the choroid and photoreceptors, delaying the regeneration of photopigments. The speed of retinal dark adaptation...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.02.007
更新日期：2019-04-01 00:00:00
abstract：:Addition of substance P (10(-7) to 10(-6) M) to rabbit iris sphincter muscle induced: (a) a rapid phosphodiesteratic breakdown of phosphatidylinositol 4,5-bisphosphate (PIP2) into 1,2-diacylglycerol (DG), measured as phosphatidic acid, and inositol triphosphate (IP3), measured by anion-exchange chromatography; (b) a r...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(86)80089-6
更新日期：1986-08-01 00:00:00
abstract：:An aqueous solution of a pigmented glucoside associated with human lens protein, 2-amino-3-hydroxyacetophenone-O-beta-D-glucoside (AHA-Glc), was irradiated with near UV-light. The near UV-irradiated glucoside was shown to generate a much lower level of active species of molecular oxygen as compared to the level of the...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2004.09.004
更新日期：2004-12-01 00:00:00
abstract：:The purpose of this study was to investigate the effects of soluble VEGF on human choroidal endothelial cell (CEC) transmigration across an RPE monolayer as it relates to choroidal neovascularization in AMD. In coculture assays, ARPE-19 (ARPE) was plated on the undersides of Transwell inserts having 0.4 microm pores. ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.08.021
更新日期：2006-04-01 00:00:00
abstract：:Innervated myotendinous cylinders (IMCs) in rabbit extraocular muscles (EOMs) were identified for the first time. The nature of IMC nerve terminals was demonstrated by means of electron microscopy and double fluorescent staining. The distal EOM portions of four rabbits of different age and sex were prepared for transm...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1085
更新日期：2001-12-01 00:00:00
abstract：:A number of variants of the wild-type (wt) estrogen receptor beta (ERbeta-1) coexist in a wide range of tissues. In the human these include, together with others, the expression of several isoforms (ERbeta-2-ERbeta-5) due to alternative splicing of exons encoding the carboxy terminus. In this study, we determined whet...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.01.019
更新日期：2005-08-01 00:00:00
abstract：:The goal of this study was to investigate the efflux of K(+) from human corneal limbal epithelial cells (HCLE) exposed to ambient levels of UVB, which is known to cause apoptosis, and to examine the effect of K(+) channel blockers on loss of potassium induced by UVB. HCLE cells were exposed to 100-200 mJ/cm(2) UVB, fo...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.02.019
更新日期：2011-05-01 00:00:00
abstract：:A panel of anti-rhodopsin monoclonal antibodies (MAbs) of defined epitope specificity has been evaluated by immunocytochemistry. Most of the IgG class MAbs (23/27) gave positive results, but only a few of the IgM class MAbs (2/21) were useful for this application. MAbs specific to the N-terminal region stained rod out...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(89)80022-3
更新日期：1989-12-01 00:00:00
abstract：:Nerve growth factor in the tear film and corneal epithelium is hypothesized to play an important role in ocular surface maintenance and corneal wound healing. The purpose of this study was to determine the expression of nerve growth factor and its high affinity (trkA) receptor in tears, cornea, and lacrimal glands of ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2004.11.013
更新日期：2005-05-01 00:00:00
abstract：:Evidence is presented that alpha-crystallin isolated at 37 degrees C exists as a species, alpha m, which has a minimum molecular weight of about 320000 and a sedimentation coefficient of about 12 S. The amino acid composition, subunit distribution, near- and far-UV CD spectra and immunochemical properties were identic...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90173-2
更新日期：1983-10-01 00:00:00
abstract：:Many ocular surface tests have been developed for the clinical diagnosis of keratoconjunctivitis sicca, and several have been used to 'validate' animal models of Sjogren's and non-Sjogren's dry eye. However, many of these tests themselves have not been systematically studied or standardized, and yet their use in anima...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2004.07.002
更新日期：2004-11-01 00:00:00
abstract：:Functional atrophy and accompanying lymphocytic infiltration and destruction of the lacrimal gland (LG) are characteristics of Sjögren's Syndrome (SjS). The male NOD mouse is an experimental model for the autoimmune exocrinopathy that develops in the LG of SjS patients. Acinar cells in LG of male NOD mice aged 3-4 mon...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.03.020
更新日期：2009-09-01 00:00:00
abstract：:We previously demonstrated that there are two waves of increased collagen synthesis following corneal laceration in rabbits. In the present study, we have examined whether increases in collagen synthesis and degradation result from increased amounts of mRNAs for collagen and collagenase, respectively. Rabbits were ane...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90086-t
更新日期：1991-03-01 00:00:00
abstract：:Non-invasive imaging is an invaluable diagnostic tool in ophthalmology. Two imaging devices, the scanning laser ophthalmoscope (SLO) and spectral domain optical coherence tomography (SDOCT), emerged from the clinical realm to provide research scientists with a real-time view of ocular morphology in living animals. We ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.10.001
更新日期：2016-12-01 00:00:00
abstract：:The integrity of the retinal pigment epithelium, especially that of the macula is essential for the preservation of vision into old age. The chronic exposure to sunlight and peroxidized lipids from phagocytized photoreceptor outer segments imposes a high level of oxidative stress on the retinal tissues, which increase...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1146
更新日期：2002-06-01 00:00:00
abstract：:We have studied the growth of neurites from single retinal ganglion cells isolated from adult goldfish and maintained under various primary cell culture conditions. In 10% Leibovitz's L-15 medium at 23 degrees C, these ganglion cells remained viable for up to 10 days and generated extensive fields of neurites. We foun...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90257-f
更新日期：1991-02-01 00:00:00
abstract：:Paired human lenses ranging from 2 to 96 yr old (n = 17) were harvested within 2 hr post-mortem and immersed in liquid nitrogen. Phosphorus containing metabolites from perchloric acid extracts were quantitated using phosphorus-31 nuclear magnetic resonance spectroscopy. Low-energy metabolites detected include the phos...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90016-8
更新日期：1991-06-01 00:00:00
abstract：:Thymic stromal lymphopoietin (TSLP) is an interleukin 7 (IL-7)-like four helix bundle cytokine that plays diverse roles in the regulation of immune responses. In fungal infection, pattern recognition receptors (PRRs), including the cell surface Toll-like receptors (TLRs) and cytoplasmic NOD-like receptors, recognize p...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.02.020
更新日期：2019-05-01 00:00:00
abstract：:We describe a model of acute intraocular pressure (IOP) elevation in the mouse eye that induces reversible loss of inner retinal function associated with oxidative stress, glial cell activation and minimal loss of retinal ganglion cell (RGC) number. Young healthy mouse eyes recover inner retinal function within 7-days...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.03.006
更新日期：2015-12-01 00:00:00
abstract：:Recent studies indicate a key role of Mitf (microphthalmia-associated transcription factor) in the differentiation of the retinal pigment epithelium (RPE). To explore transdifferentiation processes, we used small interfering RNA (siRNA) targeting Mitf. Transfection of embryonic chick RPE cells with a non-silencing flu...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.01.004
更新日期：2005-07-01 00:00:00
abstract：:Corneal autofluorescence, as measured with a commercial scanning fluorophotometer (lambda(exc): 415-491 nm; lambda(em): 515-630 nm), is increased in patients with diabetes mellitus. However, such fluorophotometers register an average fluorescence signal over all corneal layers as a consequence of their limited axial r...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0733
更新日期：1999-11-01 00:00:00
abstract：:Transport of circulating reduced glutathione (GSH) was studied at the basolateral side of the lens epithelium by using an in situ vascular eye perfusion technique in guinea-pigs with rapid sampling to ensure detecting initial uptakes. The unidirectional transport rates of [35S]-GSH (4 nM) from plasma and aqueous into ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0004
更新日期：1996-01-01 00:00:00
abstract：:Accurate thermal models for the cornea of the eye support the development of thermal techniques for reshaping the cornea and other scientific purposes. Heat transfer in the cornea must be quantified accurately so that a thermal treatment does not destroy the endothelial layer, which cannot regenerate, and yet is respo...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.07.029
更新日期：2013-10-01 00:00:00
abstract：:This review summarizes the Ernst H. Bárány Prize Lecture given at the meeting of the International Society of Eye Research in Geneva 2002. In the paper the path from the author's early studies on neurogenic inflammation in the eye to the search for a suitable prostaglandin analogue for glaucoma treatment, and the deve...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2003.11.016
更新日期：2004-04-01 00:00:00
