Abstract：
:Paired human lenses ranging from 2 to 96 yr old (n = 17) were harvested within 2 hr post-mortem and immersed in liquid nitrogen. Phosphorus containing metabolites from perchloric acid extracts were quantitated using phosphorus-31 nuclear magnetic resonance spectroscopy. Low-energy metabolites detected include the phosphomonoesters (PME) hexose-6 phosphate, alpha-glycerol phosphate, fructose 1,6-diphosphate, beta-glycerol phosphate (beta-GP), uncharacterized phosphomonoesters at 4.63 delta, 4.34 delta and 3.05 delta, phosphorylethanolamine (PE), inosine and adenosine monophosphates, and phosphorylcholine (PC). Inorganic orthophosphate (Pi), glucose 1-phosphate and glycerol 3-phosphorylcholine were the other low-energy metabolites detected. High-energy metabolites phosphocreatine (PCr), adenosine tri- and di-phosphates (ATP, ADP), nucleoside diphosphorylsugars (NS) and the dinucleotides were also detected. The following metabolic indices were calculated: PCr/Pi, PME/Pi; ATP/Pi, PCr/ATP, ATP/ADP, PE + PC, PC/PE, energy charge, phosphorylation potential, and the energy modulus. A significant linear increase with age (P less than 0.05) occurred only in the uncharacterized resonance at 3.05 delta (r = 0.4593). Significant linear decreases with age (P less than 0.05) occurred in the uncharacterized resonance at 4.63 delta (r = -0.5950), beta-GP (r = -0.5018), PCr (r = -0.4495), N.S. (r = -0.4882). There was a significant (P less than 0.05) linear decrease in the energy modulus (ratio of high-energy to low-energy metabolites).
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Merchant TE,Lass JH,Meneses P,Greiner JV,Glonek Tdoi
10.1016/0014-4835(91)90016-8subject
Has Abstractpub_date
1991-06-01 00:00:00pages
641-6issue
6eissn
0014-4835issn
1096-0007pii
0014-4835(91)90016-8journal_volume
52pub_type
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journal_title：Experimental eye research
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更新日期：1990-03-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2015-04-01 00:00:00
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更新日期：2019-08-01 00:00:00
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journal_title：Experimental eye research
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更新日期：1985-09-01 00:00:00
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更新日期：1988-03-01 00:00:00
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更新日期：1986-12-01 00:00:00
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更新日期：2001-09-01 00:00:00
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更新日期：1997-12-01 00:00:00
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更新日期：1991-08-01 00:00:00
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更新日期：1997-03-01 00:00:00
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abstract：:Retinal pigment epithelial cells (RPEs) are thought to be one of the main components of fibrous membrane observed in eyes with proliferative vitreo-retinopathy. We investigated the signalling mechanisms of TGF-beta2-dependent collagen gel contraction by RPEs. An in vitro type I collagen gel contraction assay was perfo...
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doi：10.1016/j.exer.2005.09.024
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abstract：:Transforming growth factor β (TGFβ) signaling is one of the most important signaling pathways regulating cell behavior in ocular tissues. Its functions are mainly linked to tissue fibrosis and inflammatory responses in ophthalmology. In epithelial cells, however, the growth inhibitory activity of TGFβ was reported in ...
journal_title：Experimental eye research
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journal_title：Experimental eye research
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更新日期：1992-10-01 00:00:00
abstract：:The purpose of the study was to determine histologic findings after successful laser trabeculoplasty in eyes with glaucoma. Previous studies have examined eyes in which laser did not lower intraocular pressure, or examined only the region of the laser burn itself. Nineteen autopsy eyes from 14 donors with either prima...
journal_title：Experimental eye research
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更新日期：2007-10-01 00:00:00
abstract：:The use of murine 3T3 feeder cells needs to be avoided when fabricating corneal epithelial cell sheets for use in treating ocular surface diseases. However, the expression level of the epithelial stem/progenitor cell marker, p63, is down-regulated in feeder-free culture systems. In this study, in order to fabricate co...
journal_title：Experimental eye research
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更新日期：2014-01-01 00:00:00
abstract：:Iron accumulation in the retina is associated with the development of age-related macular degeneration (AMD). IV iron is a common method to treat iron deficiency anemia in adults, and its retinal manifestations have not hitherto been identified. To assess whether IV iron formulations can be retina-toxic, we generated ...
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更新日期：2016-10-01 00:00:00
