Abstract：
:The extracellular matrix (ECM) plays a significant role in cellular and retinal health. The study of retinal tyrosine-sulfated proteins is an important first step toward understanding the role of ECM in retinal health and diseases. These secreted proteins are members of the retinal ECM. Tyrosine sulfation was shown to be necessary for the development of proper retinal structure and function. The importance of tyrosine sulfation is further demonstrated by the evolutionary presence of tyrosylprotein sulfotransferases, enzymes that catalyze proteins' tyrosine sulfation, and the compensatory abilities of these enzymes. Research has identified four tyrosine-sulfated retinal proteins: fibulin 2, vitronectin, complement factor H (CFH), and opticin. Vitronectin and CFH regulate the activation of the complement system and are involved in the etiology of some cases of age-related macular degeneration. Analysis of the role of tyrosine sulfation in fibulin function showed that sulfation influences the protein's ability to regulate growth and migration. Although opticin was recently shown to exhibit anti-angiogenic properties, it is not yet determined what role sulfation plays in that function. Future studies focusing on identifying all of the tyrosine-sulfated retinal proteins would be instrumental in determining the impact of sulfation on retinal protein function in retinal homeostasis and diseases.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Kanan Y,Al-Ubaidi MRdoi
10.1016/j.exer.2014.07.007subject
Has Abstractpub_date
2015-04-01 00:00:00pages
126-31eissn
0014-4835issn
1096-0007pii
S0014-4835(14)00189-4journal_volume
133pub_type
杂志文章,评审abstract：BACKGROUND:The laser-induced choroidal neovascularization (CNV) mouse model, as the most classic animal model of age-related macular degeneration (AMD), has been widely used. We designed a hand-held mouse holder to optimize mouse fixation in the laser-induced CNV modelling process, which was inconvenient until now. Thi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108392
更新日期：2020-12-15 00:00:00
abstract：:The purpose of this study was to investigate the influence of hyperoxia and hypercapnia on fundus pulsations in the macular and optic disc region and determine a potential autoregulative capacity of the choroid under these conditions. In a randomized cross-over study 100% O2 and a mixture of 5% CO2 with air was inhale...
journal_title：Experimental eye research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0014-4835(05)80019-3
更新日期：1995-12-01 00:00:00
abstract：:We have investigated: (a) Phospholipid composition and phosphoinositide-PO4 turnover in rabbit cornea tissues; and (b) the effects of adrenergic and serotonergic agonists on breakdown of phosphoinositides in the rabbit cornea. The data obtained from these studies can be summarized as follows: (1) in the cornea phospha...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80047-7
更新日期：1987-06-01 00:00:00
abstract：:The suitability of the rat derived SV-40T immortalized RPE-J cell line for identifying proteome changes associated with RPE differentiation was evaluated by surveying changes in protein expression levels. Rat RPE-J cells were induced to undergo differentiation in culture by growth at the nonpermissive temperature of 4...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1058
更新日期：2001-10-01 00:00:00
abstract：:An attachment to (Haag-Streit and Topcon) slit-lamps is described. This permits projection of a patterned target into the eye and the visual or photographic analysis of the details of the image reflected from the posterior surface of the crystalline lens. A numerical assessment can then be made of the optical quality ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90124-b
更新日期：1992-09-01 00:00:00
abstract：:Intraocular pressure (IOP) is the most critical risk factor for primary open angle glaucoma (POAG). In most cases of POAG, IOP is increased because of an abnormally high aqueous humor outflow resistance in the juxtacanalicular region of the trabecular meshwork. A distinct structural change in the trabecular meshwork o...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2009.01.005
更新日期：2009-04-01 00:00:00
abstract：:Abnormal migration and proliferation of endothelial cells (EC) drive neovascular retinopathies. While anti-VEGF treatment slows progression, pathology is often supported by decrease in intraocular pigment epithelium-derived factor (PEDF), an endogenous inhibitor of angiogenesis. A surface helical 34-mer peptide of PED...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107798
更新日期：2019-11-01 00:00:00
abstract：:An extraneous exon-skipping mRNA encodes an altered form of a light-absorbing opsin in human retina and pigment epithelium (RPE). The predicted protein variant differs from full-length RPE-retinal G protein-coupled receptor (RGR) by having an in-frame deletion of exon 6, which contains the entire sixth transmembrane d...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.11.013
更新日期：2006-07-01 00:00:00
abstract：:The evaluation of morphological changes of the acini in the meibomian glands is important for the diagnosis and management of dry eye related to meibomian gland dysfunction. While several tools have been developed to detect meibomian gland structure, infrared imaging is generally used in clinical settings. Unlike the ...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2017.07.005
更新日期：2017-10-01 00:00:00
abstract：:In normal retina the developing photoreceptor cells first show presence of opsin over the distal ends of the ciliary protrusions. In a fully differentiated cell intense activity is seen over the rod outer-segment discs; some activity is also seen over the Golgi zone and near the distal ends of the inner segments but t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80170-7
更新日期：1987-03-01 00:00:00
abstract：:The purpose of this study was to examine the effects of myopia-inducing and myopia recovery conditions on the scleral biomechanics of enucleated eyes of young chicks. Enucleated eyes from 5-day old chicks, with fiducial markers attached at 5 locations on the external sclera, were placed in a custom-built chamber fille...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.07.004
更新日期：2014-10-01 00:00:00
abstract：:Form-deprivation of chicks during early postnatal development results in ocular enlargement and great myopic refractive error (form-deprivation myopia). Previous studies have indicated that the retina, RPE and choroid play important roles in ocular enlargement in form-deprivation myopia. We aimed to isolate genes up-r...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0765
更新日期：2000-02-01 00:00:00
abstract：:The use of murine 3T3 feeder cells needs to be avoided when fabricating corneal epithelial cell sheets for use in treating ocular surface diseases. However, the expression level of the epithelial stem/progenitor cell marker, p63, is down-regulated in feeder-free culture systems. In this study, in order to fabricate co...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.10.019
更新日期：2014-01-01 00:00:00
abstract：:Adipose-Derived Stem Cells (ADSCs) have an important contribution in regenerative medicine ranging from testing stem cell therapy for disease treatment in pre-clinical models to clinical trials. For immediate use of stem cells for therapy, there is a requirement of the high dose of stem cells at different time points ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107860
更新日期：2019-12-01 00:00:00
abstract：:Liquid protein diets have been widely used in the United States to control weight gain. Pregnant rats were fed a standard casein diet in which liquid protein was substituted for the protein portion; all other necessary nutrients were supplied. The average incidence of cataracts in the offspring from mothers fed liquid...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90071-4
更新日期：1983-08-01 00:00:00
abstract：:Diabetic retinopathy (DR) is the most common cause of blindness in the working age population. Early events of DR are accompanied by neurodegeneration of the inner retina resulting in ganglion cell loss. These findings together with reduced retinal thickness are observed within the first weeks of experimental DR. Besi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.05.013
更新日期：2018-09-01 00:00:00
abstract：:The development of a synthetic material allowing increased cellular adhesion and ingrowth would improve keratoprosthetic devices and requires an understanding of cell colonization processes. Interlamellar implantation of hydrophobic synthetic material may lead to poor corneal nutrition with subsequent necrosis. The pr...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1193
更新日期：1994-01-01 00:00:00
abstract：:Accumulation of specific deposits and extracellular molecules under the retinal pigment epithelium (RPE) has been previously observed in eyes with age-related macular degeneration (AMD) and may play a role in the pathogenesis of AMD. Even though age is the major determinant for developing AMD, clinical studies have re...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.02.020
更新日期：2009-06-15 00:00:00
abstract：:Cochlin deposits were observed in the trabecular meshwork (TM) of 8-month-old glaucomatous DBA/2J mice, coincident with the reported onset of increased intraocular pressure and optic nerve damage. An age-dependent increase in cochlin was observed up to 10 months of age and was paralleled by a decrease in type II colla...
journal_title：Experimental eye research
pub_type： 信件
doi：10.1016/j.exer.2005.01.028
更新日期：2005-05-01 00:00:00
abstract：:Exfoliation syndrome (XFS) is an important risk factor for glaucoma (XFG) worldwide. LOXL1 variants are highly associated with XFS in most populations; however, the high frequency of risk alleles in normal individuals and the reversal of risk alleles in different ethnic populations suggest that other factors contribut...
journal_title：Experimental eye research
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.exer.2015.08.004
更新日期：2015-10-01 00:00:00
abstract：:The purpose of this study was to investigate the influence of molecular shape, conformability, net surface charge and tissue interaction on transscleral diffusion. Unfixed, porcine sclera was clamped in an Ussing chamber. Fluorophore-labelled neutral albumin, neutral dextran, or neutral ficoll were placed in one hemi-...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.07.007
更新日期：2012-09-01 00:00:00
abstract：:Deficient cGMP-phosphodiesterase (cGMP-PDE) activity results in elevated levels of cGMP in the rd retina before any pathological signs are observed. Since the enzyme is present in rd retina, although it is barely activated by light, we determined whether its synthesis starts at the same time as that of cGMP-PDE in nor...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(88)80026-5
更新日期：1988-03-01 00:00:00
abstract：:Oxyradicals probably play a major role in a number of specific pathological conditions of intraocular tissues, such as cataract formation and retinal degeneration. This paper reviews some of the mechanistic concepts relating to tissue damage by highly reactive oxidants derived from endogenous precursors, hydrogen pero...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90075-7
更新日期：1984-12-01 00:00:00
abstract：:Our previous gene expression analysis suggested that conjunctival epithelial cells of Sjögren's syndrome (SS) are inclined to hyper-proliferation and keratinisation status. The goal of this study is to elucidate whether such pathological situations really exist in the conjunctival epithelium of SS. Also, involvement o...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.07.009
更新日期：2006-03-01 00:00:00
abstract：:The Royal College of Surgeons (RCS) rat is an established animal model of retinitis pigmentosa, a family of inherited retinal diseases which starts with loss of peripheral vision and progresses to eventual blindness. Blood flow (BF), an important physiological parameter, is intricately coupled to metabolic function un...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.06.006
更新日期：2012-08-01 00:00:00
abstract：:Argon laser energy was applied to the trabecular meshwork of pigmented rabbits in an attempt to develop an animal model of 'glaucoma'. Laser energy was varied to determine the optimal level needed to produce sustained ocular hypertension. An initial response of ocular hypertension followed by hypotension was observed ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(86)90067-9
更新日期：1986-12-01 00:00:00
abstract：:Post-translational modifications by transglutaminase may contribute to the remodeling of cellular architecture in the development of lens fiber cells, and there is evidence that the enzyme may also play a role in cataract formation. It catalyses hydrolytic deamidations as well as amide exchanges on select glutamine si...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0463
更新日期：1998-05-01 00:00:00
abstract：:Thrombospondin-1 (TSP-1) is a multifunctional matrix protein that has recently been examined in various wound processes, primarily for its ability to activate the latent complex of transforming growth factor-beta (TGF-β). TGF-β has been shown to play a major role in stimulating mesenchymal cells to synthesize extracel...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.06.018
更新日期：2011-10-01 00:00:00
abstract：:Experiments were conducted to evaluate the role played by photoreceptor cells in the accumulation of age pigment, or lipofuscin, in the retinal pigment epithelium (RPE). The age-related accumulation of RPE lipofuscin was compared between rats with hereditary photoreceptor degeneration (RDY) and congenic rats with norm...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(86)80023-9
更新日期：1986-10-01 00:00:00
abstract：:In this study, we examined calcium-mediated degradation of a neurofilament protein (NFP), and autolytic activation of calpain in Lewis rat optic nerve in vitro. After incubation with calcium, homogenized optic nerve samples were analysed by SDS-PAGE in association with ECL immunoblot techniques. 68 kD NFP, calpain, an...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0286
更新日期：1997-07-01 00:00:00
