Abstract:
:Exfoliation syndrome (XFS) is an important risk factor for glaucoma (XFG) worldwide. LOXL1 variants are highly associated with XFS in most populations; however, the high frequency of risk alleles in normal individuals and the reversal of risk alleles in different ethnic populations suggest that other factors contribute to XFS pathogenesis. Clusterin (CLU) is an extracellular matrix chaperone that prevents protein aggregation and is highly expressed in ocular tissues affected by XFS. Studies examining common CLU variants for association with XFS have been inconsistent. The purpose of this study was to evaluate CLU variants for association with XFS in two independent datasets from the United States (222 cases and 344 controls) and Israel (92 cases and 102 controls). Seven tag SNPs that captured >95% of alleles at r(2) greater than 0.8 across the CLU genomic region were genotyped using TaqMan assays. Genotypes for an additional SNP, rs2279590, were imputed using phased haplotypes of HapMap reference CEU samples. Of the 8 CLU SNPs selected for the study, none were significantly associated with XFS in either case-control group (age and sex adjusted P > 0.14 and 0.36, respectively, in the US and Israeli datasets), or when they were meta-analyzed together (age and sex adjusted P > 0.13). Haplotype analysis using all 8 SNPs or only the promoter region SNPs also did not show significant associations of CLU with XFS in the combined US and Israeli dataset (P > 0.28). Meta-analysis of the data from this study and previous studies in Caucasian populations (1184 cases and 978 controls) resulted in statistically significant association of rs2279590 with XFS (summary OR = 1.18, 95% CI: 1.03-1.33, P = 0.01). Significant association between rs2279590 and XFS was also found in Indian populations (summary OR = 0.76, 95% CI: 0.61-0.96; P = 0.02); however, significant heterogeneity between the Caucasian and Indian populations possibly due to reversal of the risk allele precluded an overall meta-analysis for rs2279590 (Q = 0.001, I(2) = 91%). No significant association was identified for rs3087554 in either Caucasian populations (summary OR = 0.90, 95% CI: 0.77-1.05, P = 0.17) or Indian populations (summary OR = 0.89, 95% CI: 0.72-1.10, P = 0.28), or in both populations combined (1705 cases and 3713 controls; summary OR = 0.90, 95% CI: 0.79-1.01, P = 0.08). Significant heterogeneity precluded the addition of the Japanese data to the meta-analysis for rs3087554 (Q = 0.006, I(2) = 87%). Our results suggest that common CLU variants may contribute to modest XFS risk but even larger datasets are required to confirm these findings.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Fan BJ,Pasquale LR,Kang JH,Levkovitch-Verbin H,Haines JL,Wiggs JLdoi
10.1016/j.exer.2015.08.004subject
Has Abstractpub_date
2015-10-01 00:00:00pages
115-22eissn
0014-4835issn
1096-0007pii
S0014-4835(15)30001-4journal_volume
139pub_type
杂志文章,meta分析,评审abstract::The histopathological features of uveitis and retinal vasculitis in acute experimental allergic encephalomyelitis (EAE) were investigated using light and electron microscopy. Lewis rats were immunized by spinal cord homogenate, complete Freund's adjuvant and Bordetella pertussis. The eyes of rats with EAE exhibited va...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1024
更新日期:1993-02-01 00:00:00
abstract::In the ophthalmological clinic, intravitreally applied antibodies or Fc-containing fusion proteins are frequently used, but the biology and pharmacokinetics of these therapeutics in the retina are not well understood. We have previously shown intracellular uptake of Fc-containing molecules in RPE cells. In this study,...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.12.013
更新日期:2016-04-01 00:00:00
abstract::An aqueous solution of a pigmented glucoside associated with human lens protein, 2-amino-3-hydroxyacetophenone-O-beta-D-glucoside (AHA-Glc), was irradiated with near UV-light. The near UV-irradiated glucoside was shown to generate a much lower level of active species of molecular oxygen as compared to the level of the...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.09.004
更新日期:2004-12-01 00:00:00
abstract::Vision loss due to various forms of outer retinal degeneration remains a major problem in clinical ophthalmology. Most retinal degenerations are precipitated by genetic mutations affecting the retinal pigment epithelium and sensory retina, but it is becoming increasingly evident that resultant metabolic changes within...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2005.01.018
更新日期:2005-06-01 00:00:00
abstract::Metabolic disturbances in diabetes mellitus include changes in the type and concentration of lipids in the blood plasma which may contribute to the development of diabetic retinopathy. This disease is characterized by changes in retinal blood flow secondary to changes in the tone of retinal arterioles which is regulat...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.06.013
更新日期:2013-10-01 00:00:00
abstract::The Publisher regrets that this article is an accidental duplication of an article that has already been published, doi:10.1016/j.exer.2010.03.008. The duplicate article has therefore been withdrawn. ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2011.07.012
更新日期:2011-07-29 00:00:00
abstract::Ion channels and ion transporters play essential roles in the function of the retinal pigment epithelium (RPE). The use of cell cultures has been exploited as a key method for successfully identifying and studying ion channels and transporters of the RPE. Cultured RPE cells enable robust and long-lasting patch-clamp r...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2014.05.005
更新日期:2014-09-01 00:00:00
abstract:PURPOSE:To characterize multilamellar bodies (MLBs), determine their distribution along the optic axis and predict their potential Mie scattering within human age-related nuclear cataracts. Previous studies restricted to the equatorial plane have shown that MLBs are rare spherical objects that are 1-4 microm in diamete...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.05.017
更新日期:2004-10-01 00:00:00
abstract::A potential role for myosin light chain kinase (MLCK) in regulating intraocular pressure and outflow function has recently been reported in living monkey eye and rabbit eye. There is little information about the effects of the ocular hypotensive agents, prostaglandin F2alpha (PGF2alpha) and latanoprost on this signali...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2003.10.015
更新日期:2004-02-01 00:00:00
abstract::Human retinal pigmented epithelial (RPE) cells cultured on plastic, unlike RPE in situ, often fail to density arrest, do not produce melanin, and do not express mRNA for cellular retinaldehyde binding protein (CRALBP). When human RPE are cultured on the extracellular matrix produced by bovine corneal endothelial cells...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1158
更新日期:1993-11-01 00:00:00
abstract::The rd (retinal degeneration) chick possesses an autosomal recessive mutation which results in behavioral and electrophysiological blindness at hatch. Using the technique of two-dimensional gel electrophoresis, we have identified two groups of proteins whose expression in the retina/pigment epithelium/choroid (RET/PE/...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(89)80061-2
更新日期:1989-10-01 00:00:00
abstract::Diabetic retinopathy shares many characteristics features of a low grade chronic inflammatory disease. Its progression resists arrest when good metabolic control is re-established after a period of poor metabolic control, suggesting a 'metabolic memory' phenomenon. The aim of this study is to investigate the effect of...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2010.02.006
更新日期:2010-05-01 00:00:00
abstract::Corneal epithelial injury induces release of endogenous metabolites that are cannabinoid receptor 1 (CB1) and transient receptor potential vanilloid 1 (TRPV1) agonists. We determined the functional contributions by CB1 and TRPV1 activation to eliciting responses underlying wound healing in human corneal epithelial cel...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2010.06.022
更新日期:2010-09-01 00:00:00
abstract::Beta-crystallins are major protein constituents of the mammalian lens, where their stability and association into higher order complexes are critical for lens clarity and refraction. Dimerization is an initial step in formation of beta-crystallin complexes. Beta-crystallin association into dimers is energetically high...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2004.06.011
更新日期:2004-09-01 00:00:00
abstract::Utilizing cultured lenses from normal and homozygous glutathione peroxidase (GSHPx-1) knockout mice and inhibitors for GSSG Reductase (GSSG Red), 1,3-bis(2-chlorethyl)-1-nitrosourea (BCNU) and catalase (Cat), 3-aminotriazole (3-AT), the ability to degrade H2O2 was examined at two H2O2 concentrations, 300 microM and 80...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0250
更新日期:1997-03-01 00:00:00
abstract::Retinitis Pigmentosa (RP) is an inherited disorder that may lead to blindness. In the rhodopsin S334ter-line-3 rat model of RP, the death of rods induces spatial rearrangement of cones into regular ring mosaics. Using this model, we discovered that the ring mosaics are restored to a homogeneous distribution upon appli...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.08.005
更新日期:2015-11-01 00:00:00
abstract::Loss of vision in severe retinal degenerations often is a result of photoreceptor cell or retinal pigment epithelial cell death or dysfunction. Cell replacement therapy has the potential to restore useful vision for these individuals especially after they have lost most or all of their light-sensing cells in the eye. ...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2013.07.010
更新日期:2014-06-01 00:00:00
abstract::The proliferation, migration and transdifferentiation of the remaining lens epithelial cells (LECs) after cataract surgery are a major cause of posterior capsular opacification (PCO). It has previously been reported that salmosin, a novel disintegrin, significantly inhibits solid tumor growth in mice by perturbation o...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2001.1150
更新日期:2002-05-01 00:00:00
abstract::Aim of present investigation was to study the effect of catechin and the combination of catechin and cetirizine in ovalbumin induced animal model of allergic conjunctivitis. Guinea pigs were divided into 5 groups: normal control, disease control, disease treated with catechin 100 mg/kg, disease treated with cetirizine...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.05.020
更新日期:2015-08-01 00:00:00
abstract::The development of a synthetic material allowing increased cellular adhesion and ingrowth would improve keratoprosthetic devices and requires an understanding of cell colonization processes. Interlamellar implantation of hydrophobic synthetic material may lead to poor corneal nutrition with subsequent necrosis. The pr...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1193
更新日期:1994-01-01 00:00:00
abstract::Glutamine, the end product formed by the glutamine synthetase (GS) reaction, inhibits retinal GS activity in the presence of Mn2+, but not in the presence of Mg2+. In the presence of Mg2+, Mn2+ itself inhibits retinal GS activity. Other compounds which inhibit retinal GS activity significantly are methionine sulfoximi...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(85)90109-5
更新日期:1985-01-01 00:00:00
abstract::Recoverin is a calcium-binding protein identified as an autoantigen in a paraneoplastic degenerative disease of the human retina known as cancer-associated retinopathy (CAR). In this study we investigated whether recoverin could elicit an immune response leading to the degeneration of photoreceptor cells in a rodent r...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1130
更新日期:1994-10-01 00:00:00
abstract::Intraocular pressure (IOP) is an important risk factor for glaucoma, and the response of the ONH and surrounding tissues to elevated IOP are often investigated to better understand pathophysiology. In vivo structure including that of the optic nerve head (ONH) and surrounding tissue of the eye are often assessed using...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.01.025
更新日期:2018-04-01 00:00:00
abstract::The evaluation of morphological changes of the acini in the meibomian glands is important for the diagnosis and management of dry eye related to meibomian gland dysfunction. While several tools have been developed to detect meibomian gland structure, infrared imaging is generally used in clinical settings. Unlike the ...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2017.07.005
更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:Indirect traumatic optic neuropathy (ITON) is a major cause of permanent loss of vision after blunt head trauma. Neuroinflammation plays a crucial role in neurodegenerative diseases. The present study concentrated on JNK/c-Jun-driven NLRP3 inflammasome activation in microglia during the degeneration of retin...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.108335
更新日期:2021-01-01 00:00:00
abstract::The R49C mutation of alphaA-crystallin (alphaA-R49C) causes hereditary cataracts in humans; patients in a four-generation Caucasian family were found be heterozygous for this autosomal dominant mutation. We previously generated knock-in mouse models of this mutation and found that by 2 months of age, heterozygous muta...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2010.02.009
更新日期:2010-06-01 00:00:00
abstract::The corneal endothelial monolayer helps maintain corneal transparency through its barrier and ionic "pump" functions. This transparency function can become compromised, resulting in a critical loss in endothelial cell density (ECD), corneal edema, bullous keratopathy, and loss of visual acuity. Although penetrating ke...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2011.08.014
更新日期:2012-02-01 00:00:00
abstract::Aldose reductase (AR) is implicated in some of the disabling complications of diabetes, including neuropathy, retinopathy and cataracts. Our studies are aimed at further clarifying the role of AR in diabetes and facilitating the design of new classes of potent, specific AR inhibitors by gaining an understanding of the...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(89)90048-1
更新日期:1989-09-01 00:00:00
abstract::The effect of acute exposure to various intensities of white light on visual behavior and retinal structure was evaluated in the T4R RHO dog, a naturally-occurring model of autosomal dominant retinitis pigmentosa due to a mutation in the Rhodopsin gene. A total of 14 dogs (ages: 4-5.5 months) were used in this study: ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2016.04.006
更新日期:2016-05-01 00:00:00
abstract::The purpose of this study was to quantify how the elastic modulus of the ex vivo iris changes following stimulation by pilocarpine (PILO), phenylephrine (PE), and tropicamide (TROP). Irides (n = 20) were dissected from porcine eyes within 4 h post-mortem and tested uniaxially. Either the entire iris or sector thereof ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2009.04.014
更新日期:2009-10-01 00:00:00