Abstract：
:The development of a synthetic material allowing increased cellular adhesion and ingrowth would improve keratoprosthetic devices and requires an understanding of cell colonization processes. Interlamellar implantation of hydrophobic synthetic material may lead to poor corneal nutrition with subsequent necrosis. The probability of necrosis increases with material which is more impermeable, larger in diameter and more anterior in its placement. In this study we demonstrated the importance of pore diameter in the rate and density of cell colonization. We demonstrated that an opaque hydrophobic material may become translucent and wettable with very low perturbation of flow through the cornea. We used an expanded polytetrafluoroethylene with either 20 microns or 50 microns pore diameter. Cellular ingrowth was significantly greater in the material with 50 microns pores, resulting in collagen deposition within the pores without corneal vascularization at 6 months follow-up. Immunohistochemical study with monoclonal antibody AE5 revealed normal epithelial differentiation on the surface of the cornea over the implanted polymer.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Legeais JM,Renard G,Parel JM,Serdarevic O,Mei-Mui M,Pouliquen Ydoi
10.1006/exer.1994.1193subject
Has Abstractpub_date
1994-01-01 00:00:00pages
41-51issue
1eissn
0014-4835issn
1096-0007pii
S0014-4835(84)71193-6journal_volume
58pub_type
杂志文章abstract：:To trace the path taken by the putative postganglionic secretomotor fibres to the lacrimal gland the contents of the orbital and pterygopalatine fossa were removed whole, cut coronally into slabs and embedded in resin. Thin sections were cut at varying intervals to reconstruct the pathway taken. One group of rami orbi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2003.06.001
更新日期：2004-03-01 00:00:00
abstract：:A small percentage of retinal ganglion cells (RGCs) express melanopsin and are intrinsically photosensitive (ipRGCs). Whether light can affect the development of ipRGCs is not clear. In the rat retina, we found constant light exposure during the first postnatal week significantly increased the number of melanopsin imm...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.03.011
更新日期：2013-06-01 00:00:00
abstract：:The aquaporins (AQPs) are a family of more than 10 homologous water transporting proteins expressed in many mammalian epithelia and endothelia. At least five AQPs are expressed in the eye: AQP0 (MIP) in lens fiber, AQP1 in cornea endothelium, ciliary and lens epithelia and trabecular meshwork, AQP3 in conjunctiva, AQP...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/s0014-4835(02)00303-2
更新日期：2003-02-01 00:00:00
abstract：:Suzuki et al. [Vis. Res. 26, 425-9 (1986); Vis. Res. 28, 1061-70 (1988)] have described a formaldehyde-based (HCHO-based) extraction procedure that efficiently recovers 11-cis retinal initially present as rhodopsin chromophore in photoreceptor membranes. Using the isolated retina of the toad (Bufo marinus), we tested ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0331
更新日期：1997-09-01 00:00:00
abstract：:Interleukin-23 (IL-23) is a heterodimeric cytokine that consists of p19, a novel subunit, and p40, which is shared by IL-12. IL-23 has been demonstrated to play an important role in autoimmunity and tumor growth. However, the role of IL-23 in ocular neovascularization (NV) diseases remains unclear. In this study, we e...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.02.008
更新日期：2016-05-01 00:00:00
abstract：:A technology has recently been developed that allows for the rapid transduction of full-length functionally active proteins into intact tissue through intravenous injection and into cultured cells. This technology involves the fusion of an 11 amino acid sequence of the HIV TAT protein to the protein of interest. In th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2003.12.010
更新日期：2004-05-01 00:00:00
abstract：:Utilizing a human beta-actin promoter, a catalase cDNA expression vector was constructed. This construct was used to transfect two immortal cell lines, mouse alpha TN4-1 and rabbit N/N 1003A. The catalase activity was increased about 3.4 fold in the alpha TN4-1 cells and 38 fold in the N/N 1003A cells. Some changes in...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0560
更新日期：1998-12-01 00:00:00
abstract：:The ability of interphotoreceptor retinoid-binding protein (IRBP) to facilitate the incorporation of retinol into retinyl esters by the retinal pigment epithelium (RPE) was examined in toad (Bufo marinus) eyecup preparations devoid of neural retina (RPE-eyecup). Solutions containing purified bovine IRBP and all-trans[...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(89)80059-4
更新日期：1989-10-01 00:00:00
abstract：:Identification of causal genetic defects for human diseases took a significant leap when the first generation DNA sequencing technologies enabled biologists extract sequence-based genetic information from living beings. However, these sequencing methods had unavoidable constraints of throughput, scalability, rapidity,...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2017.08.013
更新日期：2017-11-01 00:00:00
abstract：:Isobutylmethylxanthine (IBMX), a phosphodiesterase/adenosine receptor inhibitor, was combined with norepinephrine (nE), epinephrine (Epi) and isoproterenol, respectively, to evaluate their effect on intraocular pressure (IOP). Application of topical IBMX alone had no measurable effect on IOP. When IBMX was combined wi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90051-f
更新日期：1991-05-01 00:00:00
abstract：:There has been a drive to develop new cell based therapies to treat corneal blindness, one of the most common causes of blindness worldwide. Mechanical and physical cues are known to regulate the behavior of many cell types, however studies examining these effects on corneal epithelial cells have been limited in numbe...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2018.08.001
更新日期：2018-12-01 00:00:00
abstract：:Giant papillae, characteristic lesions of vernal keratoconjunctivitis, are formed as a result of the proliferation of conjunctival fibroblasts, the deposition of extracellular matrix, and the infiltration of inflammatory cells. The concentration of interleukin (IL)-4 is also increased in the tear fluid of individuals ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(02)00248-8
更新日期：2003-01-01 00:00:00
abstract：:Microvascular dysfunctions due to altered interactions between endothelial cells (ECs) and pericytes are key-events in the pathogenesis of diabetic retinopathy. Extracellular vesicles (EVs) derived from mesenchymal stem cells cultured in diabetic-like conditions enter pericytes, cause their detachment and migration, a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.04.015
更新日期：2019-07-01 00:00:00
abstract：:The mechanisms by which sulfhydryl (SH-) binding reagents modulate the light-sensitive conductance of retinal rods were investigated by current recording from single rods, by patch clamp recording from the plasma membrane of the rod outer segment (ROS), and by biochemical study of their effects on the light-induced hy...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90176-u
更新日期：1990-07-01 00:00:00
abstract：:The ocular hypotensive effect of topically applied prostaglandins (PGs) is well documented. Although PGs introduced in the posterior chamber accumulate in the anterior tissues (e.g. iris/ciliary complex), little is known about the metabolism of PGs by these tissues. We have recently found that non-pigmented epithelial...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90202-4
更新日期：1992-08-01 00:00:00
abstract：:The purpose of this study was to compare younger and older mice after chronic intraocular pressure (IOP) elevation lasting up to 4 days with respect to mitochondrial density, structure, and movement, as well as axonal integrity, in an ex vivo explant model. We studied 2 transgenic mouse strains, both on a C57BL/6J bac...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.04.001
更新日期：2018-07-01 00:00:00
abstract：:Transport of circulating sulfur amino acids (SAA) into the lens epithelium and de novo glutathione (GSH) synthesis were studied in the perfused guinea-pig eye. Plasma-to-aqueous transfer of SAA was in their intact form (> or = 98%) and comparable with sucrose (an extracellular marker) within 30 min. The unidirectional...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0260
更新日期：1997-04-01 00:00:00
abstract：:Whole mounts of lens epithelia from rats fed on a galactose-rich diet for one, two, three or seven days were examined for morphological alterations. The meridional rows of epithelia from lenses of animals fed on galactose for seven days were found to be grossly disorganized or abnormally elongated. There was some indi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90183-0
更新日期：1984-06-01 00:00:00
abstract：:The purpose of the study was to determine histologic findings after successful laser trabeculoplasty in eyes with glaucoma. Previous studies have examined eyes in which laser did not lower intraocular pressure, or examined only the region of the laser burn itself. Nineteen autopsy eyes from 14 donors with either prima...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.07.009
更新日期：2007-10-01 00:00:00
abstract：:Sildenafil citrate increases ocular blood flow and accelerates the rate of anterior chamber refilling after paracentesis. The latter effect could have resulted from a reduction in outflow facility or from an increase in aqueous humor (AH) production. In this study, we used scanning ocular fluorophotometry to examine t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.03.016
更新日期：2013-06-01 00:00:00
abstract：:miRNA-7a plays critical roles in various biological aspects in health and disease. We aimed to reveal roles of miR-7a in mouse retinal development by loss- and gain-of-function analyses of miR-7a. Plasmids encoding miR-7a or miR-7a-decoy (anti-sense miR-7a) were introduced into mouse retina at P0, and the retina was c...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.06.022
更新日期：2015-09-01 00:00:00
abstract：:Photoreceptor cell pathology was investigated in an 8-yr-old mixed-breed dog which had displayed visual symptoms of 1 month duration. An electroretinogram detected no light-evoked responses. Light and electron microscopic features showed marked thinning and atrophy of the outer both the tapetal and non-tapetal retina ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90095-v
更新日期：1991-03-01 00:00:00
abstract：:The hyaluronan content during wound healing following excimer laser photoablation was investigated. Rabbit corneas were photoablated (Summit Omnimed, 193 nm, 5 mm diameter, 50 microns and 100 microns depth). Central optical zones of photoablated and contralateral corneas were removed 7, 30 and 90 days after surgery. C...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0212
更新日期：1997-03-01 00:00:00
abstract：:In ocular surface inflammatory diseases, such as dry eye disease, long-term symptom relief requires targeting the inflammation itself rather than treating only the surface-associated dryness with artificial tears. Therefore, we included an anti-inflammatory agent in an unpreserved liposome-based (LP) formulation used ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.11.010
更新日期：2017-01-01 00:00:00
abstract：:The aim of this study was to develop consistently focal elevated choroidal masses of human choroidal melanoma in immunosuppressed rabbits and to correlate the visualization of prognostically significant microcirculation patterns from confocal indocyanine green angiography with histologic microcirculation patterns. A h...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0650
更新日期：1999-06-01 00:00:00
abstract：:Retinitis Pigmentosa (RP) is an inherited disorder that may lead to blindness. In the rhodopsin S334ter-line-3 rat model of RP, the death of rods induces spatial rearrangement of cones into regular ring mosaics. Using this model, we discovered that the ring mosaics are restored to a homogeneous distribution upon appli...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.08.005
更新日期：2015-11-01 00:00:00
abstract：:Understanding mechanisms of glaucomatous optic nerve damage is essential for developing effective therapies to augment conventional pressure-lowering treatments. This requires that we understand not only the physical forces in play, but the cellular responses that translate these forces into axonal injury. The former ...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2010.08.005
更新日期：2011-08-01 00:00:00
abstract：PURPOSE:Cellular therapy with mesenchymal stem cells (MSC) is emerging as an effective option to treat optic neuropathies. In models of retinal degeneration, MSC injected in the vitreous body protects injured retinal ganglion cells and stimulate their regeneration, however the mechanism is still unknown. Considering th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108212
更新日期：2020-11-01 00:00:00
abstract：:Aquaporin-0 (AQP0), the major integral membrane protein in lens fiber cells, becomes highly modified with increasing age. The functional consequences of these modifications are being revealed, and the next step is to determine how these modifications affect the ocular lens, which is directly related to their abundance...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.10.007
更新日期：2011-12-01 00:00:00
abstract：:The purpose of this study was to investigate the influence of hyperoxia and hypercapnia on fundus pulsations in the macular and optic disc region and determine a potential autoregulative capacity of the choroid under these conditions. In a randomized cross-over study 100% O2 and a mixture of 5% CO2 with air was inhale...
journal_title：Experimental eye research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0014-4835(05)80019-3
更新日期：1995-12-01 00:00:00
