Abstract：
:Giant papillae, characteristic lesions of vernal keratoconjunctivitis, are formed as a result of the proliferation of conjunctival fibroblasts, the deposition of extracellular matrix, and the infiltration of inflammatory cells. The concentration of interleukin (IL)-4 is also increased in the tear fluid of individuals with ocular allergic diseases. The possible role of IL-4 in the development of giant papillae was investigated by examining the effects of this cytokine on cultured human conjunctival fibroblasts. Reverse transcription and polymerase chain reaction analysis revealed the presence of transcripts encoding the IL-4 receptor alpha chain in these cells, and flow cytometry demonstrated the expression of this protein on the cell surface. IL-4 induced the proliferation of conjunctival fibroblasts in a concentration-dependent manner, and this effect was inhibited by neutralizing antibodies to the IL-4 receptor. Enzyme immunoassays revealed that IL-4 also increased in a concentration-dependent manner the amounts of procollagen type I C-peptide and fibronectin released into the culture supernatant by conjunctival fibroblasts. A whole-cell enzyme-linked immunosorbent assay showed that IL-4 increased the deposition of collagen type III by conjunctival fibroblasts. Furthermore, reverse transcription combined with real-time polymerase chain reaction analysis revealed that IL-4 increased the abundance of collagen type III mRNA in these cells. These results demonstrate that human conjunctival fibroblasts express receptors for IL-4, and that IL-4 stimulates both the proliferation of and the production of extracellular matrix proteins by these cells. These effects of IL-4 might contribute to the formation of giant papillae in individuals with vernal keratoconjunctivitis.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Fujitsu Y,Fukuda K,Kumagai N,Nishida Tdoi
10.1016/s0014-4835(02)00248-8keywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
107-14issue
1eissn
0014-4835issn
1096-0007pii
S0014483502002488journal_volume
76pub_type
杂志文章abstract：:Beta-crystallins are major protein constituents of the mammalian lens, where their stability and association into higher order complexes are critical for lens clarity and refraction. Dimerization is an initial step in formation of beta-crystallin complexes. Beta-crystallin association into dimers is energetically high...
journal_title：Experimental eye research
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pub_type： 杂志文章
doi：10.1016/0014-4835(83)90026-x
更新日期：1983-11-01 00:00:00
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doi：10.1016/0014-4835(90)90015-m
更新日期：1990-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0014-4835(85)90150-2
更新日期：1985-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1006/exer.1993.1034
更新日期：1993-03-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2020-04-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(85)80017-8
更新日期：1985-09-01 00:00:00
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更新日期：2020-01-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0261
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pub_type： 杂志文章,评审
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更新日期：2017-03-01 00:00:00
abstract：:The purpose of this study was to determine the aqueous levels and pharmacokinetics of topical fluconazole 0.2% upon single and multiple drop applications. Forty-nine patients undergoing cataract surgery were given topical fluconazole 0.2%. They either received single drop or a loading dose of 1 drop per 5 min for 20 m...
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更新日期：2001-02-01 00:00:00
