Abstract：
:Steady state photoreceptor specific mRNA levels in bovine retina were studied during fetal maturation for five gene transcripts, including rhodopsin, arrestin (S-antigen), rod alpha-subunit of transducin, interphotoreceptor retinoid-binding protein (IRBP) and rod alpha-subunit of cGMP phosphodiesterase in order to understand mechanisms of gene regulation during photoreceptor development. A 10-15-fold increase in each transcript level begins between 5.5 and 6 months of gestation for each gene, suggesting a single coordinate induction event at this time. Quantitative analysis of transcriptional rates for each gene by nuclear run-on also reveals a coordinate increase at approximately the same time, demonstrating that induction is achieved by transcriptional activation. Interestingly, however, gene specific transcription rates in the pre-induction retina do not appear to parallel mRNA steady state levels. During fetal development neither the transcript level of each gene relative to the others nor relative mRNA turnover rates change substantially after the induction event at 5.5-6.0 months. However, at earlier times all genes exhibit higher mRNA turnover, implying that differential mRNA stability may also play an important role in determining steady-state levels.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Timmers AM,Newton BR,Hauswirth WWdoi
10.1006/exer.1993.1034subject
Has Abstractpub_date
1993-03-01 00:00:00pages
257-65issue
3eissn
0014-4835issn
1096-0007pii
S0014-4835(83)71034-1journal_volume
56pub_type
杂志文章abstract：:The anterior avian cornea possesses several distinct cellular and extracellular regions including the epithelial basal lamina, Bowman's layer and the interfacial matrix that separates Bowman's layer from the stroma. These unique regions differ biochemically, physically and morphologically but all contain type XII coll...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2002.2058
更新日期：2002-12-01 00:00:00
abstract：:We have compared IOP elevations induced by water-loading and by increased cephalic venous pressure in normal and denervated pigmented rabbits. Denervations were performed by sympathetic ganglionectomy and/or blockade of the sensory and autonomic innervation of the eye through retrobulbar anesthesia; retrobulbar anesth...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(89)80005-3
更新日期：1989-03-01 00:00:00
abstract：:Rat retinas were labeled either by intravitreal injection of [14C]leucine or by incubation with [3H]-leucine or [35S]-methionine. Subcellular fractions were prepared on linear sucrose gradients and rhodopsin was extracted with detergent and purified by chromatography on ConA-Sepharose. A fraction enriched in rough end...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(89)90007-9
更新日期：1989-05-01 00:00:00
abstract：:Polyclonal antisera have been made to synthetic peptides corresponding to the N- and C-terminal regions of the putative gamma 1-2 gene from human lens. These antisera are specific for gamma crystallin, showing no cross-reactivity with any polypeptides of the alpha- and beta-crystallin fractions. Western blot analysis ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80144-6
更新日期：1987-08-01 00:00:00
abstract：:Mammalian lens development involves cell-cell and cell-ECM interactions. As integrins are a major family of cell adhesion molecules, we examined the expression patterns of several integrin subunits (alpha3A, alpha3B, alpha6A, alpha6B, beta1 and beta4) during rat lens development. RT-PCR, in situ hybridisation, immunof...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.02.005
更新日期：2005-09-01 00:00:00
abstract：:Thioltransferase (TTase) activity was identified and partially purified from the ocular tissue for the first time. The enzyme activity depended on the presence of reduced glutathione (GSH), glutathione reductase (GR) and NADPH to reduce the disulfide bond in a synthetic substrate, hydroxyl ethyl disulfide (HEDS). Maxi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0133
更新日期：1996-10-01 00:00:00
abstract：:The aim of the study was to evaluate the antiproliferative and cytotoxic properties of triamcinolone acetonide (TA) on human retinal pigment epithelium cells (ARPE19) and the role of epicellular crystalline deposits. Monolayer cultures of ARPE19 cells were used. Purified or unpurified crystalline TA suspension (0.01-1...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.02.012
更新日期：2006-09-01 00:00:00
abstract：:Uncoupling protein 2 (UCP2) is a mitochondrial transporter present in the inner membrane of mitochondria, and it uncouples substrate oxidation from ATP synthesis, thereby dissipating the membrane potential energy and consequently decreasing ATP production by mitochondrial respiratory chain. As a consequence of the unc...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.11.004
更新日期：2012-01-01 00:00:00
abstract：:AII amacrine cells are critical interneurons in the rod pathway of mammalian retina, active primarily in dim lighting conditions. Melatonin, a neuromodulator produced at night in the retina, is believed to induce retinal adaptation to dim lighting conditions in most vertebrate species examined to date, including human...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(03)00123-4
更新日期：2003-09-01 00:00:00
abstract：:TGFβ2 induces extracellular matrix (ECM) remodeling and alters the cytoskeleton by both the canonical Smad and non-canonical signaling pathways. TGFβ2 regulates the expression of ECM proteins in trabecular meshwork (TM) cells, increases intraocular pressure (IOP) in an ex vivo perfusion organ culture model, and induce...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2013.10.017
更新日期：2013-11-01 00:00:00
abstract：:To apply nuclear magnetic resonance (NMR) spectroscopy to study the penetration of dexamethasone phosphate into the aqueous humour from rabbit following topical administration. After topical administration of 0.1%, 1.0% and 10% dexamethasone phosphate solutions, respectively, samples of aqueous humour were aspirated, ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0429
更新日期：1998-03-01 00:00:00
abstract：:The shapes of two human lenses of different ages (27 and 46 years old) were altered by applying a stretching force to the ciliary muscle. The force was in the outward radial direction and was produced by an electronically driven stretching instrument which is described. The lenses were photographed for different degre...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1168
更新日期：1993-11-01 00:00:00
abstract：:Macromolecular cell markers are essential for the classification and characterization of the highly complex and cellularly diverse vertebrate retina. Although a plethora of markers are described in the current literature, the immunoreactivity of these markers in normal human tissue has not been fully determined. This ...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2016.01.002
更新日期：2016-09-01 00:00:00
abstract：:All aqueous humor draining through the conventional outflow pathway must cross the endothelium of Schlemm's canal (SC), likely by passing through micron-sized transendothelial pores. SC pores are non-uniformly distributed along the inner wall endothelium, but it is unclear how the distribution of pores relates to the ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.11.008
更新日期：2015-01-01 00:00:00
abstract：:Previous studies suggest that an alpha 2-related isoform of the catalytic subunit is predominant in the lens fiber cells. The alpha 1 isoform is predominant in the lens epithelium (Garner, Horwitz and Enomoto, 1992). Data are presented to show that strophanthidin-sensitive K+ transport is sustained by both of these le...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1068
更新日期：1994-06-01 00:00:00
abstract：:The gene for microphthalmia in the homozygous cinnamon mouse produces colobomatous microphthalmia due to failure of closure of the optic fissure. Optic fissure formation and closure were examined in control cinnamon and homozygous microphthalmic foetuses from the 10th to the 13th gestational day using light and electr...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(89)90093-6
更新日期：1989-08-01 00:00:00
abstract：:The expression and intracellular localization of the estrogen receptor (ER) subtypes, ERalpha and ERbeta were examined in cultured human lens epithelial cells (HLE-B3). ERalpha and ERbeta mRNA expression was evaluated by coupled reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot analysis. Subce...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2003.09.027
更新日期：2004-04-01 00:00:00
abstract：:We have examined the mercapturic acid pathway of the cataractous rabbit lens following induction by naphthalene as an oxidative foreign substance. 1,2-Naphthoquinone, which is formed in the eye from naphthalene diol and other naphthalene derivatives by a combination of enzymic and non-enzymic reactions, readily oxidiz...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(88)90058-9
更新日期：1988-09-01 00:00:00
abstract：:The identification of genes that modify pathological ocular phenotypes in mouse models may improve our understanding of disease mechanisms and lead to new treatment strategies. Here, we identify modifier loci affecting photoreceptor cell loss in homozygous Mfrp(rd6) mice, which exhibit a slowly progressive photorecept...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.10.020
更新日期：2014-01-01 00:00:00
abstract：:The development of a synthetic material allowing increased cellular adhesion and ingrowth would improve keratoprosthetic devices and requires an understanding of cell colonization processes. Interlamellar implantation of hydrophobic synthetic material may lead to poor corneal nutrition with subsequent necrosis. The pr...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1193
更新日期：1994-01-01 00:00:00
abstract：:Autoradiographic studies showed selective accumulation of [3H]-glycine, [3H]-GABA, and the GABA agonist [3H]-muscimol by neurons of the mudpuppy retina. [3H]-Glycine was taken up by bipolar cells, amacrine cells, and displaced amacrine or ganglion cells. Both [3H]-GABA and [3H]-muscimol were also accumulated by bipola...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90116-7
更新日期：1984-07-01 00:00:00
abstract：:The transport kinetics across the plasma-ocular barriers of labeled molecules including urea, D-glucose, 3-0-methyl-D-glucose, L-glucose, and sucrose were studied in adult RCS rats and compared to control albino Sprague-Dawleys (SD). Results indicate that substances that passively cross the plasma-aqueous and plasma-v...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90156-2
更新日期：1983-09-01 00:00:00
abstract：:Lens epithelium-derived growth factor (LEDGF) has been shown to enhance survival of lens epithelial cells (LECs) against stress. The objectives of these studies are to determine how LEDGF controls PKC gamma activity in normal LECs: how this control of PKC gamma regulates the phosphorylation of Connexin 43, the inhibit...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(03)00049-6
更新日期：2003-05-01 00:00:00
abstract：:Post-translational modifications and/or structural changes induced by modifications are likely causes of the decrease in crystallin solubility associated with aging and the development of cataract. Characterization of human lens crystallins by mass spectrometry has demonstrated that betaB2-crystallin undergoes less mo...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1023
更新日期：2001-08-01 00:00:00
abstract：:The retinal pigment epithelium is a transporting epithelium that helps regulate the volume and composition of the subretinal space surrounding photoreceptor outer segments. The capacity of the RPE to actively transport Na+ and K+ between the retina and the blood supply depends on the localization of the Na+, K(+)-ATPa...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1144
更新日期：1994-11-01 00:00:00
abstract：:Familial exudative vitreoretinopathy (FEVR) is a disease exhibits a wide range of clinical signs, ranging mild peripheral retinal vascular anomalies to severe retinal detachments. Individuals with mild FEVR are frequently asymptomatic with good visual function and are often undiagnosed. However, little is known about ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.107941
更新日期：2020-03-01 00:00:00
abstract：:miRs play critical roles in oxidative stress-related retinopathy pathogenesis. miR-365 was identified in a previously constructed library from glyoxal-treated rat Müller cell. This report explores epigenetic alterations in Müller cells under oxidative stress to develop a novel therapeutic strategy. To examine the miR-...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.11.006
更新日期：2018-03-01 00:00:00
abstract：:Two low-Ca2+-requiring proteases (calpain I) and one high-Ca2+-requiring protease (calpain II) have been separated from the cytosol of rat retina by DEAE-cellulose column chromatography. Calpain I was half-maximally activated at 3 microM free Ca2+ and fully activated at 10 microM free Ca2+. Half-maximal activation of ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(85)90098-3
更新日期：1985-07-01 00:00:00
abstract：:Aceclidine can dissociate accommodative and outflow facility responses in monkeys and humans. We sought to determine if different muscarinic receptor subtypes control outflow facility, accommodative and pupillary responses to aceclidine in the living rhesus monkey eye, as a possible basis for this separation. Each eye...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1057
更新日期：1994-05-01 00:00:00
abstract：:Sugar alcohols have been reported to accumulate in retinal pigment epithelium (RPE) of diabetic animals. This finding has raised interest in the role of RPE in diabetes-associated retinal changes such as cystoid macular edema. To confirm the presence of aldose reductase in this tissue, the NADPH-dependent enzyme was p...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1119
更新日期：1993-08-01 00:00:00
