Abstract:
:Polyclonal antisera have been made to synthetic peptides corresponding to the N- and C-terminal regions of the putative gamma 1-2 gene from human lens. These antisera are specific for gamma crystallin, showing no cross-reactivity with any polypeptides of the alpha- and beta-crystallin fractions. Western blot analysis demonstrates a dramatic decrease in the binding of these antisera to gamma crystallin during aging of the normal human lens, while identical analysis with polyclonal antisera to the major cyanogen bromide fragment (19,000 MW) of human gamma crystallin shows little, if any, change during aging. Together, these demonstrate that antisera to synthetic peptides of the N- and C-terminal regions of the gamma 1-2 gene are very specific probes that can demonstrate extensive covalent modification from both ends of the gamma crystallin molecule during aging of the normal human lens.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Takemoto L,Kodama T,Takemoto Ddoi
10.1016/s0014-4835(87)80144-6subject
Has Abstractpub_date
1987-08-01 00:00:00pages
207-14issue
2eissn
0014-4835issn
1096-0007journal_volume
45pub_type
杂志文章abstract::The zebrafish retina regenerates in response to acute retinal lesions, replacing damaged neurons with new neurons. In this study we test the hypothesis that chronic stress to inner retinal neurons also triggers a retinal regeneration response in the bugeye zebrafish. Mutations in the lrp2 gene in zebrafish are associa...
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journal_title:Experimental eye research
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