Abstract:
:The mechanisms by which sulfhydryl (SH-) binding reagents modulate the light-sensitive conductance of retinal rods were investigated by current recording from single rods, by patch clamp recording from the plasma membrane of the rod outer segment (ROS), and by biochemical study of their effects on the light-induced hydrolysis of cyclic GMP. The electrophysiology, as well as measurements of the reagents' ability to traverse the ROS plasma membrane, was done on amphibian (Rana and Ambystoma) rods, and the biochemistry on bovine rods. The main SH-reagents used were N-ethyl-maleimide (NEM) and iodoacetamide (IAA). Both transiently increased rod current, but part of the large current could not be turned off by light. After a few minutes' exposure, NEM, but not IAA, caused a continuous decay of the rod's light sensitivity. In patch-clamp recordings from the ROS plasma membrane, the reagents increased conductivity both in the presence and absence of cGMP, consistent with the observation that the drug-induced current increase in intact rods involved both light-sensitive and light-insensitive components. In vitro, NEM was found to be a powerful inhibitor of cGMP hydrolysis, which can explain the gradual loss of light sensitivity in the rod and could initially contribute to the increased dark current via elevated cGMP levels. Thus, SH-reagents act both by modifying the light-sensitive channel and by inhibiting phototransduction inside the rod.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Donner K,Hemilä S,Kalamkarov G,Koskelainen A,Pogozheva I,Rebrik Tdoi
10.1016/0014-4835(90)90176-usubject
Has Abstractpub_date
1990-07-01 00:00:00pages
97-105issue
1eissn
0014-4835issn
1096-0007pii
0014-4835(90)90176-Ujournal_volume
51pub_type
杂志文章abstract::A technology has recently been developed that allows for the rapid transduction of full-length functionally active proteins into intact tissue through intravenous injection and into cultured cells. This technology involves the fusion of an 11 amino acid sequence of the HIV TAT protein to the protein of interest. In th...
journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2004-05-01 00:00:00
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
pub_type: 杂志文章
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2004.02.017
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0194
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(91)90095-v
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:1989-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
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更新日期:2016-03-01 00:00:00
abstract::The function of acetylcholinesterase (AChE) is to terminate the action of acetylcholine at the cholinergic synapse. Recent evidence suggests additional roles for acetylcholinesterase as a peptidase and/or a protease which is expressed by growing neurites as part of their invasion of developing neural structures. We re...
journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:1995-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:1985-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
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更新日期:2013-11-01 00:00:00
abstract::The elimination of spermine, the end product of cellular polyamine, from the retina to the blood across the blood-retinal barrier (BRB) was investigated. The in vivo microdialysis study revealed that the elimination of [(3)H]spermine from vitreous humor after vitreous bolus injection was in a biexponential manner. The...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2014.05.002
更新日期:2014-07-01 00:00:00