Abstract：
:The polypeptides in portions of human lens epithelium from individuals of various ages were resolved by two-dimensional (2D) gel electrophoresis. The epithelium was divided into a central area of 12.5 mm2, a surrounding area of 50.2 mm2, and an area which was outside of this region. The proteins were solubilized in urea and run on gels to determine if differences existed in the major polypeptides of the lens epithelium with regard to position in the lens as well as if differences might occur with age. The patterns obtained were remarkably similar in regions of the epithelium examined. The results also demonstrated that the composition of the major polypeptides were not substantially altered in the adult epithelium with age. Two-dimensional gels of proteins from a 3-month-old specimen prepared in a manner similar to that used during extracapsular cataract extraction were also similar to the other samples; however, this sample contained slightly increased staining of proteins greater than 30 kD.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Russell Pdoi
10.1016/0014-4835(91)90064-lsubject
Has Abstractpub_date
1991-05-01 00:00:00pages
613-8issue
5eissn
0014-4835issn
1096-0007pii
0014-4835(91)90064-Ljournal_volume
52pub_type
杂志文章abstract：:We investigated the inhibitory action of a synthetic peptidyl hydroxamate inhibitor of matrix metalloproteinase (MMP), Galardin (GM6001), on collagen degradation by rabbit corneal stromal fibroblasts (keratocytes) cultured three-dimensionally in the type I collagen gel with medium containing interleukin 1alpha (IL-1al...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0637
更新日期：1999-05-01 00:00:00
abstract：:Deficient cGMP-phosphodiesterase (cGMP-PDE) activity results in elevated levels of cGMP in the rd retina before any pathological signs are observed. Since the enzyme is present in rd retina, although it is barely activated by light, we determined whether its synthesis starts at the same time as that of cGMP-PDE in nor...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(88)80026-5
更新日期：1988-03-01 00:00:00
abstract：:The signal transduction pathways initiated by Ca(2+)-mobilizing agonists, such as prostaglandin F(2alpha)(PGF(2alpha)) and carbachol (CCh), leading to activation of cytosolic phospholipase A(2)(cPLA(2)) and arachidonic acid (AA) release in a wide variety of tissues remain obscure. To further define the role of protein...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.0991
更新日期：2001-05-01 00:00:00
abstract：:Glutamate is an excitatory neurotransmitter in the central and peripheral nervous systems of the vertebrate. The previous studies show the presence of mRNAs of AMPA-type glutamate receptors, GluR1 and GluR2, in the optic tectum of the pigeon, suggesting glutamatergic input from the retina. The present study examined l...
journal_title：Experimental eye research
pub_type： 信件
doi：10.1016/j.exer.2009.03.024
更新日期：2009-09-01 00:00:00
abstract：:The presence of the alpha(1)-adrenoceptor subtypes in various parts of the pig and rabbit eyes was investigated using [(3)H]-prazosin radioligand binding. The characterization of the subtypes was achieved by performing competition experiments with various subtype selective drugs. In the pig retina, both alpha(1A)- and...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0753
更新日期：2000-01-01 00:00:00
abstract：:This study investigated the changes in pH during retinal artery occlusion by means of extracellular H+ concentration ([H+]o) measurements in the retina under both air and 100% O2 ventilation. Occlusion was produced in intact anesthetised cats by pressing with a probe onto a retinal artery. [H+]o profiles were recorded...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2004.11.002
更新日期：2005-04-01 00:00:00
abstract：:The ability of diinosine polyphosphates, diinosine triphosphate (Ip(3)I), diinosine tetraphosphate (Ip(4)I) and diinosine pentaphosphate (Ip(5)I) to modify intraocular pressure in normotensive New Zealand white rabbits was tested. Ip(5)I produced increase in intraocular pressure, while Ip(3)I and Ip(4)I produced a dec...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.05.011
更新日期：2012-08-01 00:00:00
abstract：:The levels of alpha 1-proteinase inhibitor (alpha 1-antitrypsin) in keratoconus, normal human, and other diseased corneas were examined. Using an immunoperoxidase technique, the presence of this inhibitor was demonstrated in the epithelium, stroma and endothelium of all corneal sections. Compared with normal human con...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90044-u
更新日期：1990-05-01 00:00:00
abstract：:The use of murine 3T3 feeder cells needs to be avoided when fabricating corneal epithelial cell sheets for use in treating ocular surface diseases. However, the expression level of the epithelial stem/progenitor cell marker, p63, is down-regulated in feeder-free culture systems. In this study, in order to fabricate co...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.10.019
更新日期：2014-01-01 00:00:00
abstract：:The benzopyran BP (3,4-dihydro-6-hydroxy-7-methoxy-2,2-dimethyl-1(2H)-benzopyran) is a free radical scavenger that is structurally similar to alpha-tocopherol and has provided neuro-protection in a number of disease models where oxidative stress is a causative factor. A novel derivative of BP with improved lipid solub...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.04.007
更新日期：2011-07-01 00:00:00
abstract：:The ability of interphotoreceptor retinoid-binding protein (IRBP) to facilitate the incorporation of retinol into retinyl esters by the retinal pigment epithelium (RPE) was examined in toad (Bufo marinus) eyecup preparations devoid of neural retina (RPE-eyecup). Solutions containing purified bovine IRBP and all-trans[...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(89)80059-4
更新日期：1989-10-01 00:00:00
abstract：:By methods of subtraction-hybridization of lambda ZAP cDNA libraries, prepared from 4-week-old rat lens epithelial cells (capsule) and lens fiber cells (decapsulated lens), we have isolated a specific cDNA clone whose target mRNA is about 600 b long. Northern blot hybridization analysis data showed that the target mRN...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80009-0
更新日期：1995-06-01 00:00:00
abstract：:The immunolocalization of the low density lipoprotein receptor-related protein 1 (LRP1) and its ligand alpha 2-Macroglobulin (alpha(2)M) was examined in tissues from human donor eyes of normal, diabetic and sickle cell disease subjects. Streptavidin alkaline phosphatase immunohistochemistry was performed with a mouse ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.05.017
更新日期：2010-08-01 00:00:00
abstract：:Functional atrophy and accompanying lymphocytic infiltration and destruction of the lacrimal gland (LG) are characteristics of Sjögren's Syndrome (SjS). The male NOD mouse is an experimental model for the autoimmune exocrinopathy that develops in the LG of SjS patients. Acinar cells in LG of male NOD mice aged 3-4 mon...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.03.020
更新日期：2009-09-01 00:00:00
abstract：:An enzymatic procedure for the estimation of organic hydroperoxides has been adapted to biological tissues and applied to the measurement of hydroperoxides in the rat retina. Hydroperoxides are determined from the coupled activities of glutathione peroxidase and glutathione reductase as measured by the loss of NADPH a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90116-1
更新日期：1983-03-01 00:00:00
abstract：:The differentiation of rat lens epithelial cells to fibre cells can be mimicked using lens epithelial explants, which differentiate in vitro when exposed to fibroblast growth factor (FGF). A previous study demonstrated that FGF is required only for initiation of differentiation: once induced by FGF, differentiation ca...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0534
更新日期：1998-10-01 00:00:00
abstract：:The Purkinje cell degeneration (pcd) mutant mouse rapidly loses cerebellar Purkinje cells and about 50% of its retinal photoreceptor cells at between 3 and 5 weeks of age, and thereafter slowly loses the remaining photoreceptor cells during the first year of life. An ultrastructural study of the developing photorecept...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90019-o
更新日期：1992-05-01 00:00:00
abstract：:Utilizing a human beta-actin promoter, a catalase cDNA expression vector was constructed. This construct was used to transfect two immortal cell lines, mouse alpha TN4-1 and rabbit N/N 1003A. The catalase activity was increased about 3.4 fold in the alpha TN4-1 cells and 38 fold in the N/N 1003A cells. Some changes in...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0560
更新日期：1998-12-01 00:00:00
abstract：:The retinal pigment epithelial cell has several important functions, one of which is the phagocytosis of photoreceptor outer segments which are discarded diurnally. We previously provided evidence in human retinal pigment epithelium that CD36, an 88 kDa integral membrane glycoprotein, participates in the phagocytosis ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0177
更新日期：1997-01-01 00:00:00
abstract：:The conjunctival associated lymphoid tissue is considered to be an integral part of the mucosal immune system. Under normal circumstances immune mechanisms in mucosal associated lymphoid tissue of the gut and bronchus can selectively suppress, rather than enhance, immune responsiveness to encountered antigens, inducin...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0297
更新日期：1997-06-01 00:00:00
abstract：:Previous studies have suggested that abnormal corneal wound healing in patients after photorefractive keratectomy (PRK) is associated with the appearance of myofibroblasts in the stroma between two and four weeks after surgery. The purpose of this study was to examine potential myofibroblast progenitor cells that migh...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.02.022
更新日期：2009-08-01 00:00:00
abstract：:The aim of this study was to develop consistently focal elevated choroidal masses of human choroidal melanoma in immunosuppressed rabbits and to correlate the visualization of prognostically significant microcirculation patterns from confocal indocyanine green angiography with histologic microcirculation patterns. A h...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0650
更新日期：1999-06-01 00:00:00
abstract：:Structure and function of the retina mainly rely on its fatty acid (FA) composition. Evidence from epidemiological studies and from animal experiments indicates that FA composition of the retina is influenced by the diet. Mice under chronic high-fat diet (HFD) develop metabolic syndrome, a risk factor for diabetes tha...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108059
更新日期：2020-07-01 00:00:00
abstract：:Interphotoreceptor retinoid-binding protein (IRBP) greatly enhances the conversion of all- trans -retinol to 11- cis -retinal by the retinal pigment epithelium (RPE) and facilitates 11- cis -retinal release from the RPE. However, the mechanisms by which IRBP exerts these effects are not clear. Using a model system of ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0781
更新日期：2000-02-01 00:00:00
abstract：:Identification of novel agents to eradicate the residual lens cell population following cataract surgery provides one mode of preventing PCO formation. The present study investigated the biological mechanism of As(2)O(3) cytotoxicity in a human lens cell line and capsular bag system. FHL 124 cell survival was assessed...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.08.018
更新日期：2007-12-01 00:00:00
abstract：:Retinal arrestin is known to participate in the quenching of phototransduction through binding to light-activated and phosphorylated rhodopsin. Recently, a splice variant of retinal arrestin was identified in bovine photoreceptors which could bind unphosphorylated photoactive rhodopsin. In this report, a splice varian...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0069
更新日期：1996-06-01 00:00:00
abstract：:The gene for microphthalmia in the homozygous cinnamon mouse produces colobomatous microphthalmia due to failure of closure of the optic fissure. Optic fissure formation and closure were examined in control cinnamon and homozygous microphthalmic foetuses from the 10th to the 13th gestational day using light and electr...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(89)90093-6
更新日期：1989-08-01 00:00:00
abstract：:Post-translational modifications by transglutaminase may contribute to the remodeling of cellular architecture in the development of lens fiber cells, and there is evidence that the enzyme may also play a role in cataract formation. It catalyses hydrolytic deamidations as well as amide exchanges on select glutamine si...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0463
更新日期：1998-05-01 00:00:00
abstract：:Arf encodes an important tumor suppressor, p19(Arf), which also plays a critical role to control hyperplasia in the primary vitreous during mouse eye development. In the absence of Arf, mice are born blind and display a phenotype closely mimicking severe forms of the human eye disease, persistent hyperplastic primary ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.01.004
更新日期：2016-04-01 00:00:00
abstract：:This study tests the hypothesis that choroidal myogenic autoregulation participates in the intraocular pressure (IOP) response to mean arterial pressure (MAP) by minimizing arterial-pressure-dependent changes in choroidal blood volume. To test this hypothesis, the IOP response to MAP was quantified before and after im...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1047
更新日期：1994-05-01 00:00:00
