Abstract：
:The differentiation of rat lens epithelial cells to fibre cells can be mimicked using lens epithelial explants, which differentiate in vitro when exposed to fibroblast growth factor (FGF). A previous study demonstrated that FGF is required only for initiation of differentiation: once induced by FGF, differentiation can be maintained by insulin (as assessed by following the accumulation of fibre-cell specific crystallins). The aim of this investigation was to determine whether insulin-like growth factor 1 (IGF-1) can also maintain differentiation and to include a cellular analysis of explants undergoing insulin-or IGF-maintained differentiation in vitro. Measurement of the accumulation of alpha-, beta- and gamma-crystallins showed that IGF-1, like insulin, can replace FGF-2 in directing the pulses of alpha-, beta- and gamma-crystallin gene expression once differentiation is initiated by FGF-2. Cells in both the peripheral and the central region of the explants responded. Immunolocalization of alpha, beta- and gamma-crystallins in these explants showed that a 15 min pulse of FGF-2 triggered the differentiation of only a few cells, whereas a 12 hr pulse primed virtually all the cells for differentiation. This indicates that in explants, individual cells differ in the rate at which they can respond to FGF-2.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Klok Ej,Lubsen NH,Chamberlain CG,McAvoy JWdoi
10.1006/exer.1998.0534subject
Has Abstractpub_date
1998-10-01 00:00:00pages
425-31issue
4eissn
0014-4835issn
1096-0007pii
S0014483598905346journal_volume
67pub_type
杂志文章abstract：:The aim of this study was to identify the molecular genetic cause of disease in posterior polymorphous corneal dystrophy (PPCD) probands of diverse origin and to assess the utility of massively parallel sequencing in the detection of ZEB1 mutations. We investigated a total of 12 families (five British, four Czech, one...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.03.002
更新日期：2019-05-01 00:00:00
abstract：:Reactive astrocytes in glaucomatous optic nerve changes are characterized by an increased expression of alphaB-crystallin and transforming growth factor-beta (TGF-beta). In the pathogenesis of glaucomatous optic nerve damage, ischemia/reperfusion injury may play an important role. The goal of the present study was to ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.12.008
更新日期：2007-04-01 00:00:00
abstract：:Glaucoma is a leading cause of irreversible blindness worldwide. Lowering intraocular pressure (IOP) is the only strategy documented to delay the appearance and retard the progression of vision loss. One major approach for lowering IOP is to slow the rate of aqueous humor formation by the ciliary epithelium. As discus...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2008.11.005
更新日期：2009-04-01 00:00:00
abstract：:The presence of the alpha(1)-adrenoceptor subtypes in various parts of the pig and rabbit eyes was investigated using [(3)H]-prazosin radioligand binding. The characterization of the subtypes was achieved by performing competition experiments with various subtype selective drugs. In the pig retina, both alpha(1A)- and...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0753
更新日期：2000-01-01 00:00:00
abstract：:We sought to characterize the ocular pharmacology, tolerability and intraocular pressure (IOP)-lowering efficacy of FR-190997, a non-peptidic bradykinin (BK) B2-receptor agonist. FR-190997 possessed a relatively high receptor binding affinity (Ki = 27 nM) and a high in vitro potency (EC50 = 18.3 ± 4.4 nM) for inositol...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.10.008
更新日期：2014-11-01 00:00:00
abstract：:Immunocytochemistry with a monoclonal antibody against the 28-kDa calcium-binding protein (28-kDa CaBP) was used to study horizontal cells in both congenic control (RCS-rdy+) and dystrophic (RCS) 3-month-old rat retina. We found that horizontal cells in the RCS-rdy+ rat retinas were distributed evenly from the centre ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1109
更新日期：1993-08-01 00:00:00
abstract：:Harmonic analysis was used to characterize the rabbit flicker ERG elicited by sinusoidally modulated full-field stimuli under light-adapted conditions. The frequency-response function for fundamental amplitude, derived from Fourier analysis of the ERG waveforms, exhibited two limbs, with an amplitude minimum at approx...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.10.005
更新日期：2010-12-01 00:00:00
abstract：:Cataract is a multifactorial disease, and a large variety of stressors induce cataracts. Many cataractogenic stressors and endoplasmic reticulum (ER) stressors induce the unfolded protein response (UPR) in various cell types. The UPR is known to produce reactive oxygen species (ROS) prior to the inducement of apoptosi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.01.033
更新日期：2006-09-01 00:00:00
abstract：:Twenty-three human lenses from age 3-76 years were studied. Lenses obtained 24-36 post-mortem were exposed to monochromatic light (435.8 and 546.1 nm) in the I parallel and I+ modes and to white (tungsten lamp) unpolarized radiation. The light scattering intensities were obtained as a function of scattering angles fro...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(85)90089-2
更新日期：1985-07-01 00:00:00
abstract：:The effect of acute exposure to various intensities of white light on visual behavior and retinal structure was evaluated in the T4R RHO dog, a naturally-occurring model of autosomal dominant retinitis pigmentosa due to a mutation in the Rhodopsin gene. A total of 14 dogs (ages: 4-5.5 months) were used in this study: ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.04.006
更新日期：2016-05-01 00:00:00
abstract：:Non-invasive imaging is an invaluable diagnostic tool in ophthalmology. Two imaging devices, the scanning laser ophthalmoscope (SLO) and spectral domain optical coherence tomography (SDOCT), emerged from the clinical realm to provide research scientists with a real-time view of ocular morphology in living animals. We ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.10.001
更新日期：2016-12-01 00:00:00
abstract：:Exposure of the rabbit lens, in vitro, to 10(-3) M-hydrogen peroxide resulted in an increase of the lens sodium, calcium and water content together with a decrease of the lens potassium content. No such changes were observed in the presence of 10(-4) or 10(-5) M-hydrogen peroxide. 10(-3) M-hydrogen peroxide was observ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90148-3
更新日期：1983-07-01 00:00:00
abstract：:The current study investigates the cellular events which trigger activation of proapoptotic Bcl-2-associated × protein (Bax) in retinal cell death induced by all-trans-retinal (atRAL). Cellular events which activate Bax, such as DNA damage by oxidative stress and phosphorylation of p53, were evaluated by immunochemica...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.04.003
更新日期：2014-06-01 00:00:00
abstract：:Binocular vision provides the most accurate and precise depth information; however, many people have impairments in binocular visual function. It is possible that other sensory inputs could be used to obtain reliable depth information when binocular vision is not available. However, it is currently unknown whether dep...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.08.013
更新日期：2019-06-01 00:00:00
abstract：:The distribution of collagen degradation and synthesis (turnover) between the central and peripheral areas of clear corneal allografts was determined, following transplantation of 6-mm [3H]proline-labelled corneal buttons from extensively pre-labelled, young rabbits into 13 nonradioactive mature rabbits. Control, cont...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80076-3
更新日期：1987-07-01 00:00:00
abstract：:N-methyl-D-aspartate (NMDA)-induced excitotoxicity is an acute form of experimental retinal injury as a result of overactivation of glutamate receptors. NLRP3 (nucleotide-binding domain, leucine-rich-repeat containing family, pyrin domain containing-3) inflammasome, one of the most studied sensors of innate immunity, ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.01.018
更新日期：2019-04-01 00:00:00
abstract：:The mechanical, histological and pharmacological properties of the isolated cat ophthalmociliary artery are reported and compared for passively stretched ring segments from three locations on the artery. Ring segments were mounted in a modified myograph system after the method of Hogestatt, Andersson and Edvinsson (19...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90047-v
更新日期：1992-03-01 00:00:00
abstract：:Proliferative diabetic retinopathy (PDR) is characterized by intraocular formation of fibroglial vascularized tissue by active vasoproliferative mechanisms. Using immunocytochemistry, we have studied changes in the distribution pattern of fibronectin (FN), laminin (LN), vitronectin (VN) and their receptors in the newl...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80079-x
更新日期：1995-01-01 00:00:00
abstract：:TGFβ2 induces extracellular matrix (ECM) remodeling and alters the cytoskeleton by both the canonical Smad and non-canonical signaling pathways. TGFβ2 regulates the expression of ECM proteins in trabecular meshwork (TM) cells, increases intraocular pressure (IOP) in an ex vivo perfusion organ culture model, and induce...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2013.10.017
更新日期：2013-11-01 00:00:00
abstract：:The distribution of hyaluronan (HA) in the posterior eye wall from the vitreous through the sclera, with special consideration to localization in the retina and interphotoreceptor matrix (IPM), was evaluated in mouse tissues using an HA specific probe (bHABC, biotinylated hyaluronan binding complex). The vitreous body...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0369
更新日期：1997-11-01 00:00:00
abstract：:This review is focused on iron metabolism in the retina and in the lens and its relation to their respective age-related pathologies, macular degeneration (AMD) and cataract (ARC). Several aspects of iron homeostasis are considered first in the retina and second in the lens, paying particular attention to the transpor...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2010.03.003
更新日期：2010-06-01 00:00:00
abstract：:The corneal endothelial monolayer helps maintain corneal transparency through its barrier and ionic "pump" functions. This transparency function can become compromised, resulting in a critical loss in endothelial cell density (ECD), corneal edema, bullous keratopathy, and loss of visual acuity. Although penetrating ke...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2011.08.014
更新日期：2012-02-01 00:00:00
abstract：:Myo/Nog cells are essential for eye development in the chick embryo and respond to injury in adult tissues. These cells express mRNA for the skeletal muscle specific transcription factor MyoD, the bone morphogenetic protein (BMP) inhibitor Noggin and the cell surface protein recognized by the G8 monoclonal antibody (m...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2015.11.023
更新日期：2016-05-01 00:00:00
abstract：:Neovascular age-related macular degeneration (AMD) is a leading cause of vision loss worldwide. Although intravitreal injection of anti-VEGF antibodies and VEGF Trap have significant clinical benefits, the complications of intravitreal injection, drug resistance and patient compliance still need to be concerned. In th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.12.009
更新日期：2018-03-01 00:00:00
abstract：:The aim of this study was to develop consistently focal elevated choroidal masses of human choroidal melanoma in immunosuppressed rabbits and to correlate the visualization of prognostically significant microcirculation patterns from confocal indocyanine green angiography with histologic microcirculation patterns. A h...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0650
更新日期：1999-06-01 00:00:00
abstract：:To determine the effect of roscovitine, a potent antiviral in tissue culture administered intramuscularly to rabbits or by eye drops to mice for the treatment of herpetic keratitis this study was commenced.New Zealand white rabbits infected with McKrae strain herpesvirus (HSV-1) were treated twice a day with 10mg Rosc...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(03)00056-3
更新日期：2003-06-01 00:00:00
abstract：:The aim of the study was to investigate the molecular genetics of uveal melanoma (UM) metastases and correlate it with disease progression. Twelve pathologically confirmed UM metastases from 11 patients were included. Molecular genetic alterations in chromosomes 3 (including the BAP1 region), 8q, 6p, and 1p were inves...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.04.010
更新日期：2012-07-01 00:00:00
abstract：:Exposure to hyposmotic solution causes release of ATP from lens cells via hemichannels. Because hemichannel opening feasibly could swamp the cells with calcium, we carried out studies to measure the magnitude of the increase in cytoplasmic calcium concentration caused by hemichannel opening. In studies on porcine lens...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.01.012
更新日期：2015-03-01 00:00:00
abstract：:Monkey eyes are useful for ophthalmologic research into eye diseases because their histological and functional properties are very similar to those of humans. The monkey laser-induced high intraocular pressure (IOP) model is a common model for ophthalmologic research, especially into glaucoma. Although several studies...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.02.004
更新日期：2008-05-01 00:00:00
abstract：:Retinal arrestin is known to participate in the quenching of phototransduction through binding to light-activated and phosphorylated rhodopsin. Recently, a splice variant of retinal arrestin was identified in bovine photoreceptors which could bind unphosphorylated photoactive rhodopsin. In this report, a splice varian...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0069
更新日期：1996-06-01 00:00:00
