Abstract:
:Proliferative diabetic retinopathy (PDR) is characterized by intraocular formation of fibroglial vascularized tissue by active vasoproliferative mechanisms. Using immunocytochemistry, we have studied changes in the distribution pattern of fibronectin (FN), laminin (LN), vitronectin (VN) and their receptors in the newly-formed capillaries of PDR. In intraocular vascularized tissue of PDR patients, FN was present on both luminal and basal surfaces of endothelial cells, and was diffusely distributed in the interpericyte space. LN was also associated with the interpericyte space. VN was occasionally detected on the luminal capillary side, but was frequent in the basal aspect of the endothelium in the interpericyte space, where it was colocalized with FN. Beta-1 subunit complex receptors were detected on the luminal side, while alpha v beta 3 integrin was identified on both sides, more so in the luminal than in the basal endothelial domain. By slot-blotting techniques and densitometric analysis, increased concentrations of intravitreous FN and VN were found in PDR in comparison with normal samples. These results suggest that FN, VN and LN have a key role in the structural arrangement of newly formed capillaries in PDR, and that receptor expression could be involved in events of endothelial cell adhesion and proliferation.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Casaroli Marano RP,Preissner KT,Vilaró Sdoi
10.1016/s0014-4835(05)80079-xsubject
Has Abstractpub_date
1995-01-01 00:00:00pages
5-17issue
1eissn
0014-4835issn
1096-0007journal_volume
60pub_type
杂志文章abstract::Ultraviolet A (UVA) light-based photoactivation of riboflavin (Rf) to induce corneal crosslinking (CXL) and mechanical stiffening is now a well-known treatment for corneal ectasia and Keratoconus that is being used in a topographically guided photorefractive intrastromal CXL (PiXL) procedure to treat low degrees of re...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2020.108199
更新日期:2020-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80144-6
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1152
更新日期:1994-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1006
更新日期:1994-02-01 00:00:00
abstract::Many ocular surface tests have been developed for the clinical diagnosis of keratoconjunctivitis sicca, and several have been used to 'validate' animal models of Sjogren's and non-Sjogren's dry eye. However, many of these tests themselves have not been systematically studied or standardized, and yet their use in anima...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
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更新日期:2004-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0111
更新日期:1996-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2016.01.002
更新日期:2016-09-01 00:00:00
abstract::The purpose of this investigation is to characterize parvalbumin-immunoreactive (IR) neurons in the inner nuclear layer (INL) of zebrafish retina through immunocytochemistry, quantitative analysis, and confocal microscopy. In the INL, parvalbumin-IR neurons were located in the inner marginal portion of the INL. On the...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2008.11.014
更新日期:2009-03-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(89)80059-4
更新日期:1989-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2012.08.007
更新日期:2012-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0047
更新日期:1996-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2017.07.005
更新日期:2017-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2008.01.008
更新日期:2008-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.10.020
更新日期:2014-01-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90188-x
更新日期:1984-04-01 00:00:00
abstract::The development and growth of the vertebrate ocular lens is dependent on the regulated proliferation of an anterior monolayer of epithelial cells, and their subsequent differentiation into elongate fiber cells. The growth factor rich ocular media that bathes the lens mediates these cellular processes, and their respec...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.02.012
更新日期:2018-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2016.06.009
更新日期:2017-05-01 00:00:00
abstract::Albino mice exposed to 1 X 10(-4) W cm-2 of UV radiation in the range 290-400 nm for 12 hr per day developed anterior lens opacities within 1-2 months after onset of the exposure. Histological changes demonstrated marked derangement of the lens epithelium. When the radiation was filtered with glass, eliminating radiat...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(85)80011-7
更新日期:1985-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 临床试验,杂志文章,评审
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更新日期:1990-06-01 00:00:00
abstract::This study was performed to further characterize expression and function of the Fas/Fas ligand system in the cornea. Specifically, these experiments examined (1) the effect of genetic inactivation of Fas or Fas ligand genes on keratocyte apoptosis in response to corneal epithelial wounding, (2) whether cultured human ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0371
更新日期:1997-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2014.04.003
更新日期:2014-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.108018
更新日期:2020-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2010.07.007
更新日期:2010-10-01 00:00:00
abstract::The elimination of spermine, the end product of cellular polyamine, from the retina to the blood across the blood-retinal barrier (BRB) was investigated. The in vivo microdialysis study revealed that the elimination of [(3)H]spermine from vitreous humor after vitreous bolus injection was in a biexponential manner. The...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2014.05.002
更新日期:2014-07-01 00:00:00
abstract::Corneal epithelial injury induces release of endogenous metabolites that are cannabinoid receptor 1 (CB1) and transient receptor potential vanilloid 1 (TRPV1) agonists. We determined the functional contributions by CB1 and TRPV1 activation to eliciting responses underlying wound healing in human corneal epithelial cel...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2010.06.022
更新日期:2010-09-01 00:00:00
abstract::Previous studies in the laboratory have shown that platelet-activating factor (PAF), a potent inflammatory mediator that accumulates rapidly in the cornea after an injury, stimulates the expression of urokinase (uPA) and matrix metalloproteinase-1 (MMP-1) and -9 (MMP-9). Tissue inhibitors of MMPs (TIMPs) and plasminog...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2001.1135
更新日期:2002-03-01 00:00:00
abstract::Mounting evidence suggests neuroinflammation is a key process in glaucoma, yet the precise roles are not known. Understanding these complex processes, which may also be a key in other common neurodegenerations such as Alzheimer's disease, will lead to targeted therapeutics for a disease that affects as many as 80 mill...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2017.02.014
更新日期:2017-04-01 00:00:00
abstract::This study sought to pharmacologically characterize bradykinin receptors on SV40-immortalized human trabecular meshwork (HTM3) cells. Phosphoinositide (PI) turnover studies were conducted using [3H]myo-inositol-labeled HTM3 cells and anion exchange chromatography to quantify [3H]inositol phosphates generated in respon...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0157
更新日期:1996-12-01 00:00:00
abstract::Between 5 and 70% of genetic disease is caused by in-frame nonsense mutations, which introduce a premature termination codon (PTC) within the disease-causing gene. Consequently, during translation, non-functional or gain-of-function truncated proteins of pathological significance, are formed. Approximately 50% of all ...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2017.01.001
更新日期:2017-02-01 00:00:00
abstract::The part of the light spectrum which is responsible for an increase of lenticular green autofluorescence upon white light exposure was determined for the rabbit. The increase of autofluorescence as a function of the light energy was measured, to assess a possible threshold energy for lens alterations. Thirteen rabbits...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1045
更新日期:1993-03-01 00:00:00