Abstract：
:This study sought to pharmacologically characterize bradykinin receptors on SV40-immortalized human trabecular meshwork (HTM3) cells. Phosphoinositide (PI) turnover studies were conducted using [3H]myo-inositol-labeled HTM3 cells and anion exchange chromatography to quantify [3H]inositol phosphates generated in response to bradykinin (BK) and various BK analogs. The blockade of these responses was studied using two potent and receptor-subtype selective antagonists. BK and T-kinin (Ile-Ser-BK:TK) induced a 4.2-4.4 fold stimulation of PI turnover above base levels at 1-10 microM. Several other peptides unrelated to BK, including angiotensin II, endothelin, cholecystokinin, bombesin and peptide YY tested at 1-10 microM were essentially inactive. The molar potencies (EC50) of BK, TK and close analogs were: BK = 4.5 +/- 0.5 nM (n = 6), Lys-BK = 6.5 +/- 0.7 nM (n = 3), TK = 38.8 +/- 6.6 nM (n = 8), Met-Lys-BK = 41.5 +/- 13.4 nM (n = 4), Des-Arg9-BK = 2093 +/- 626 nM (n = 4). All the latter BK-related peptides were full agonists. The actions of BK and TK were potently and competitively antagonized by Hoe-140 (molar potency = 0.6-1 nM; pA2 = 8.97-9.21. n = 3-4) and by D-Arg0[Hyp3,-Thi5.8,-DPhe7]-BK (molar potency = 251 nM; -log potency, pKb = 6.6), two selective B2-type BK antagonists. In conclusion, rank order of potency of BK agonists and the blockade of BK- and TK-induced PI turnover by the selective antagonists are consistent with the classification of the BK receptors on HTM3 cells as the B2-receptor subtype.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Sharif NA,Xu SXdoi
10.1006/exer.1996.0157subject
Has Abstractpub_date
1996-12-01 00:00:00pages
631-7issue
6eissn
0014-4835issn
1096-0007pii
S0014-4835(96)90157-8journal_volume
63pub_type
杂志文章abstract：:The routine cultivation of human corneal endothelial cells, with the view to treating patients with endothelial dysfunction, remains a challenging task. While progress in this field has been buoyed by the proposed existence of progenitor cells for the corneal endothelium at the corneal limbus, strategies for exploitin...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.07.002
更新日期：2014-10-01 00:00:00
abstract：:Prolyl oligopeptidase (EC 3.4.21.26) has been purified 26,000-fold from bovine lens tissue by anion-exchange chromatography, gel filtration and isoelectric focussing, with an overall yield of 11%. The purified enzyme exhibited an isoelectric point of 4.8, a pH optimum of 7.5 and a molecular mass of 72 kDa under both n...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1086
更新日期：1994-07-01 00:00:00
abstract：:A perifusion system has been designed for metabolic nuclear magnetic resonance (NMR) spectroscopy studies of the bovine retina. This system, which allows the use of unmodified commercial probes for multinuclear studies, is shown here to maintain the perifused retina in a metabolic steady state at 25 degrees C, as dedu...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1175
更新日期：1993-12-01 00:00:00
abstract：:Recent studies have shown that lactate coupled water flux is the underlying mechanism of the corneal endothelial pump, which is highly dependent on the presence of bicarbonate. In this study we test the hypothesis that the increased intracellular pH (pHi) caused by bicarbonate stimulates glycolytic activity and the pr...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108193
更新日期：2020-10-01 00:00:00
abstract：:Transport of circulating sulfur amino acids (SAA) into the lens epithelium and de novo glutathione (GSH) synthesis were studied in the perfused guinea-pig eye. Plasma-to-aqueous transfer of SAA was in their intact form (> or = 98%) and comparable with sucrose (an extracellular marker) within 30 min. The unidirectional...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0260
更新日期：1997-04-01 00:00:00
abstract：:The ocular lens is surrounded by the lens capsule, which is an elastic and unusually thick basal membrane. Anionic sites are thought to be responsible for charge-selective permeability barriers in basal membranes. We have used cationic colloidal gold as a tracer for anionic binding sites to investigate the distributio...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0952
更新日期：2001-03-01 00:00:00
abstract：:Crystalline lenses excised from 5-7-month-old rabbits and pigs were extracted for lipids with chloroform/methanol, 2/1 using the Folch method. The extracted crude lipids were analyzed at 202 MHz by 31P-NMR spectroscopy. Twelve membrane phospholipids were detected. Both rabbit and pig phospholipid profiles contained ph...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90207-b
更新日期：1990-03-01 00:00:00
abstract：:Much controversy has arisen on the nature and sources of stem cells in the adult human retina. Whilst ciliary epithelium has been thought to constitute a source of neural stem cells, a population of Müller glia in the neural retina has also been shown to exhibit neurogenic characteristics. This study aimed to compare ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.09.015
更新日期：2011-12-01 00:00:00
abstract：:The lacrimal glands of male NOD mice exhibit many of the features of the human lacrimal gland in patients afflicted with the autoimmune disease, Sjögren's syndrome, including loss of secretory functions and lymphocytic infiltration into the lacrimal gland. To elucidate the early changes in the secretory pathway associ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.04.019
更新日期：2006-01-01 00:00:00
abstract：:The association between the use of statins and age-related macular degeneration (AMD), a leading cause of blindness, has been evaluated in many clinical studies; however, the results have been contradictory. We evaluated the effect of pitavastatin administration on laser-induced experimental choroidal neovascularizati...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.02.005
更新日期：2007-06-01 00:00:00
abstract：:Microaneurysms, acellular capillaries and pericyte ghosts are characteristic of diabetic retinopathy, but it is not clear what causes these lesions or whether they are causally related to each other. The distribution of microaneurysms, acellular capillaries and pericyte ghosts has been evaluated in two animal models o...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80069-7
更新日期：1995-05-01 00:00:00
abstract：:The spontaneously hypertensive rats (SHR) show a lower intraocular pressure--despite high arterial blood pressure--than the normotensive Wistar Kyoto rats (NR). In SHR the quantitative evaluation of resin casts and flat-mount preparations of the blood vessels of the ciliary body and choroid showed a significant dilati...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(85)90004-1
更新日期：1985-02-01 00:00:00
abstract：:Ischaemia/reperfusion contributes to the pathophysiological process of many retinal diseases. Previous studies have shown that retinal ischaemia/reperfusion mainly results in neuronal degeneration, including thinning of the retina, retinal ganglion cell death and reductions in electroretinography. A high-salt diet con...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107784
更新日期：2019-11-01 00:00:00
abstract：:Previous studies suggest that an alpha 2-related isoform of the catalytic subunit is predominant in the lens fiber cells. The alpha 1 isoform is predominant in the lens epithelium (Garner, Horwitz and Enomoto, 1992). Data are presented to show that strophanthidin-sensitive K+ transport is sustained by both of these le...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1068
更新日期：1994-06-01 00:00:00
abstract：:Human, monkey, and bovine retinal pigment epithelial (RPE) cells exhibit an M-type K+ current, which in many other cell types is mediated by channels composed of KCNQ α-subunits and KCNE auxiliary subunits. Recently, we demonstrated the expression of KCNQ1, KCNQ4, and KCNQ5 in the monkey RPE. Here, we investigated the...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：
更新日期：2013-11-01 00:00:00
abstract：:The effect of acute exposure to various intensities of white light on visual behavior and retinal structure was evaluated in the T4R RHO dog, a naturally-occurring model of autosomal dominant retinitis pigmentosa due to a mutation in the Rhodopsin gene. A total of 14 dogs (ages: 4-5.5 months) were used in this study: ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.04.006
更新日期：2016-05-01 00:00:00
abstract：:Oxidative stress and inflammation are important pathological mechanisms in many neurodegenerative diseases, including age-related macular degeneration (AMD). The very low-density lipoprotein receptor knockout mouse (Vldlr-/-) has been identified as a model for AMD and in particular for retinal angiomatous proliferatio...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.08.003
更新日期：2013-11-01 00:00:00
abstract：:Pericytes exert an important influence on the control of retinal blood flow; however, little is known regarding the molecular basis of retinal pericyte excitability. The purpose of this study was to elucidate the signaling pathway of how prostacyclin (PGI2), an important endogenous regulator of retinal blood flow, sti...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.07.011
更新日期：2006-12-01 00:00:00
abstract：:Developing canine retina, fixed in Bouin's or Tellyesnizky's acetic alcoholic formalin, was examined with a battery of biotinylated lectins. ConA and WGA consistently bound to all retinal layers including the tapetum cellulosum, retinal pigment epithelium, inner and outer segments, plexiform layers and ganglion cells....
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90150-s
更新日期：1990-10-01 00:00:00
abstract：:The rapid increase in popularity of extracapsular cataract extraction may predispose the eye to postoperative bacterial infections by introducing viable organisms through the additional instrumentation and irrigation necessary for the extracapsular technique. Lens protein released into the aqueous humor of the anterio...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(89)80034-x
更新日期：1989-11-01 00:00:00
abstract：:Aceclidine can dissociate accommodative and outflow facility responses in monkeys and humans. We sought to determine if different muscarinic receptor subtypes control outflow facility, accommodative and pupillary responses to aceclidine in the living rhesus monkey eye, as a possible basis for this separation. Each eye...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1057
更新日期：1994-05-01 00:00:00
abstract：:Fungal keratitis is a common disease that causes blindness. An effective animal model for fungal keratitis is essential for advancing research on this disease. Our objective is to develop a novel mouse model of Fusarium solani keratitis through the inoculation of fluorescent-labeled fungi into the cornea to facilitate...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.03.002
更新日期：2013-05-01 00:00:00
abstract：:Interphotoreceptor retinoid-binding protein (IRBP) is a vitamin A carrier present only in the extracellular material lying between the neural retina and the retinal pigment epithelium of vertebrate eyes. The amount of retinol bound endogenously by IRBP in this interphotoreceptor space is known to increase upon illumin...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90239-b
更新日期：1991-09-01 00:00:00
abstract：:Superoxide radicals or products generated by these radicals in a xanthine/xanthine oxidase system lyse cultured rat and human corneal epithelial cells as measured in a chromium-51 release assay. Partial protection from this lysis is afforded by superoxide dismutase and complete protection is obtained with catalase. Hy...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90004-e
更新日期：1990-01-01 00:00:00
abstract：:Glutamine, the end product formed by the glutamine synthetase (GS) reaction, inhibits retinal GS activity in the presence of Mn2+, but not in the presence of Mg2+. In the presence of Mg2+, Mn2+ itself inhibits retinal GS activity. Other compounds which inhibit retinal GS activity significantly are methionine sulfoximi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(85)90109-5
更新日期：1985-01-01 00:00:00
abstract：:This study tests the hypothesis that choroidal myogenic autoregulation participates in the intraocular pressure (IOP) response to mean arterial pressure (MAP) by minimizing arterial-pressure-dependent changes in choroidal blood volume. To test this hypothesis, the IOP response to MAP was quantified before and after im...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1047
更新日期：1994-05-01 00:00:00
abstract：:The cornea receives the densest sensory innervation of the body, which is exclusively from small-fiber nociceptive (pain-sensing) neurons. These are similar to those in the skin of the legs, the standard location for neurodiagnostic skin biopsies used to diagnose small-fiber peripheral polyneuropathies. Many cancer ch...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.05.016
更新日期：2013-08-01 00:00:00
abstract：:The supply of L-carnosine, a bioactive dipeptide of β-alanine and l-histidine, to the retina across the blood-retinal barrier (BRB) was studied. The in vivo and in vitro studies revealed low uptake activities for [(3)H]Gly-Sar, a representative dipeptide, suggesting that l-carnosine transport plays only a minor role a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.06.002
更新日期：2013-08-01 00:00:00
abstract：:The chelating agent Na2EDTA was perfused through the anterior chambers of monkeys eyes in an attempt to find a concentration that would cause a rise in outflow facility without harming the cornea or anterior uvea. Na2EDTA (0.5 mM) proved effective in increasing the facility within 60 min. There were marked effects on ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80002-7
更新日期：1987-02-01 00:00:00
abstract：:Rat retinas were labeled either by intravitreal injection of [14C]leucine or by incubation with [3H]-leucine or [35S]-methionine. Subcellular fractions were prepared on linear sucrose gradients and rhodopsin was extracted with detergent and purified by chromatography on ConA-Sepharose. A fraction enriched in rough end...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(89)90007-9
更新日期：1989-05-01 00:00:00
