Abstract：
:Interphotoreceptor retinoid-binding protein (IRBP) is a vitamin A carrier present only in the extracellular material lying between the neural retina and the retinal pigment epithelium of vertebrate eyes. The amount of retinol bound endogenously by IRBP in this interphotoreceptor space is known to increase upon illumination. This finding led to the hypothesis that IRBP may act as a shuttle for vitamin A during the visual cycle that regenerates rhodopsin. In the present work, we separated IRBP from other retinoid-binding proteins in bovine interphotoreceptor matrix preparations by means of size-exclusion chromatography. IRBP's endogenous ligands were retained during this procedure and were then extracted into hexane and analysed by normal-phase HPCL. We found that IRBP carries, in a light-dependent manner, all the retinoid isomers involved in the visual cycle. For dark-adapted eyes the amounts of bound ligands are (in nmol per eye) 0.09 all-trans retinol, 0.11 11-cis retinol, 0.04 all-trans retinal, 0.16 11-cis retinal, and 0.07 retinyl esters. For light-adapted eyes the amount of all-trans retinol was found to increase by a factor of five, and that of 11-cis retinal to decrease by a factor of four. (These eyes contain 3.1 nmol of IRBP, which does not change in amount with lighting conditions). Thus, the major endogenous ligand of IRBP is 11-cis retinaldehyde in the dark and all-trans retinol in the light. The data are consistent with a role for IRBP as a non-selective scavenger and stabilizer of retinoids released from photoreceptors and pigment epithelial cells. However, it cannot be concluded from these data that IRBP is involved in directed transport of retinoids across the interphotoreceptor matrix, since there is no evidence for the appropriate spatial gradients in the ligands bound to the protein.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Adler AJ,Spencer SAdoi
10.1016/0014-4835(91)90239-bsubject
Has Abstractpub_date
1991-09-01 00:00:00pages
337-46issue
3eissn
0014-4835issn
1096-0007journal_volume
53pub_type
杂志文章abstract：:An optimal system for monitoring in vivo corneal wound healing is inexpensive, has utility for wounding and imaging, and is able to provide previews before photography. We outline such an imaging system that takes advantage of a consumer digital camera and an LED-based light source for fluorescein excitation. Using FV...
journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：2007-06-01 00:00:00
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journal_title：Experimental eye research
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更新日期：2017-06-01 00:00:00
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journal_title：Experimental eye research
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doi：10.1016/s0014-4835(87)80144-6
更新日期：1987-08-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90176-u
更新日期：1990-07-01 00:00:00
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更新日期：1995-03-01 00:00:00
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更新日期：2011-11-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2012-02-01 00:00:00
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doi：10.1016/0014-4835(84)90008-3
更新日期：1984-08-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1013
更新日期：1993-01-01 00:00:00
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journal_title：Experimental eye research
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doi：10.1016/j.exer.2013.06.002
更新日期：2013-08-01 00:00:00
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doi：10.1016/j.exer.2020.108281
更新日期：2020-12-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0265
更新日期：1997-05-01 00:00:00
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doi：10.1016/j.exer.2011.12.004
更新日期：2012-01-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：2006-05-01 00:00:00
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更新日期：2006-12-01 00:00:00
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更新日期：2010-08-01 00:00:00
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更新日期：2001-11-01 00:00:00
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更新日期：1995-04-01 00:00:00
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journal_title：Experimental eye research
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doi：
更新日期：2002-10-01 00:00:00
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journal_title：Experimental eye research
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更新日期：1991-05-01 00:00:00
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更新日期：2020-11-01 00:00:00
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