Abstract：
:Neovascular eye diseases such as wet age-related macular degeneration and proliferative diabetic retinopathy are two of the most common causes of irreversible visual loss. Although mediated by vascular endothelial growth factor (VEGF), the mechanisms of these diseases are not fully understood. Molecular inhibitors of VEGF including pegaptanib, ranibizumab and bevacizumab are used as treatments for these diseases. However, there have been very few direct comparisons between these agents, and as dose and treatment regimes differ their relative efficacies are hard to determine. In vitro comparisons tend to use cells from different sites or species, which show heterogeneity in their responses. The aim of this study was to compare the characteristics of primary cultures of isolated human choroidal endothelial cells (hCEC) and retinal endothelial cells (hREC), and their proliferation responses to stimulation with VEGF 121 and 165, and to compare the anti-proliferative effects of these three drugs. hCEC and hREC were positive for the cell markers VEGFR1, VEGFR2, CD31, CD34 and von Willebrand's factor (vWF), with greater expression of CD34 on the hREC compared to hCEC. Contrary to previous assumptions VEGF isoforms 121 and 165 were found to be equally potent in stimulating endothelial cell proliferation. However, hREC exhibited higher proliferation with either VEGF isoform compared to hCEC. The anti-VEGF treatments ranibizumab and bevacizumab were effective in decreasing proliferation of hCEC induced by the two VEGF isoforms, individually and in combination, with ranibizumab being moderately more effective, particularly in hREC. Pegaptanib was effective in controlling the proliferation of hCEC stimulated by VEGF 165, but was ineffective against the stimulatory effect of VEGF 121. There were found to be significant differences in microvascular endothelial cells from the retina and choroid, both in the expression of cell markers and their behaviour in response to growth factors and currently available anti-VEGF agents, highlighting the importance of targeting treatments to specific intraocular vascular beds and/or diseases.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Stewart EA,Samaranayake GJ,Browning AC,Hopkinson A,Amoaku WMdoi
10.1016/j.exer.2011.09.010subject
Has Abstractpub_date
2011-11-01 00:00:00pages
761-6issue
5eissn
0014-4835issn
1096-0007pii
S0014-4835(11)00272-7journal_volume
93pub_type
杂志文章abstract：:Glucosyltransferase, an enzyme involved in the synthesis of the carbohydrate portion of basement membranes and collagens, and collagen content were determined in the retina of normal and streptozotocin diabetic rats. No significant difference in glucosyltransferase activity was found at 4 weeks of diabetes. However, t...
journal_title：Experimental eye research
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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更新日期：2020-04-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0147
更新日期：1996-11-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：1988-09-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90190-4
更新日期：1992-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80036-3
更新日期：1995-08-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90054-x
更新日期：1990-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.exer.2014.04.003
更新日期：2014-06-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0265
更新日期：1997-05-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2016.06.009
更新日期：2017-05-01 00:00:00
abstract：:Choroidal neovascularization (CNV) associated with the 'wet' form of age related macular degeneration (AMD) is one of the most common causes of central vision loss among the elderly. The 'wet' form of AMD is currently treated by intravitreal delivery of anti-VEGF agents. However, intravitreal injections are associated...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.09.005
更新日期：2015-11-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90124-b
更新日期：1992-09-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.01.015
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
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更新日期：2017-05-01 00:00:00
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journal_title：Experimental eye research
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更新日期：2004-03-01 00:00:00
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更新日期：2009-11-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1117
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journal_title：Experimental eye research
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更新日期：2016-05-01 00:00:00
abstract：:Several variants of growth hormone (GH) are found in the retina and vitreous of the chick embryo, where they appear to act as cell survival factors, having neuroprotective effects on retinal ganglion cells (RGCs). Here, we investigate the molecular mechanisms of the anti-apoptotic effect of GH in cultured RGCs. GH tre...
journal_title：Experimental eye research
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doi：10.1016/j.exer.2006.06.009
更新日期：2006-11-01 00:00:00
abstract：:Multidrug resistance associated protein (MRP) is a major family of efflux transporters involved in drug efflux leading to drug resistance. The objective of this study was to explore physical barriers for ocular drug absorption and to verify if the role of efflux transporters. MRP-2 is a major homologue of MRP family a...
journal_title：Experimental eye research
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doi：10.1016/j.exer.2006.08.015
更新日期：2007-01-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：1984-12-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.09.024
更新日期：2006-05-01 00:00:00
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journal_title：Experimental eye research
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doi：10.1016/j.exer.2016.03.009
更新日期：2017-03-01 00:00:00
