Abstract:
:A serum-free culture of chicken retinal pigmented epithelial cells has been established in order to analyse how cell-substrate interactions or environmental factors affect the process of transdifferentiation into lens cells from pigmented epithelial cells. The serum-free culture medium for chicken pigmented epithelial cells was Eagle's minimum essential medium, supplemented with chicken transferrin, soybean trypsin inhibitor and bovine insulin. Pigmented epithelial cells were able to survive and grow in the medium for longer than 2 weeks. Collagen did not promote initial cell attachment, but this material effectively supports pigmented epithelial cells to organize monolayer structure characteristics to pigmented epithelium in situ in comparison with the plastic substrate of culture dishes. The process of lens transdifferentiation of chicken pigmented epithelial cells in serum-free conditions was also enhanced with the aid of phenylthiourea and testicular hyaluronidase, which had already been known to promote the transdifferentiation of pigmented epithelial cells in the serum-supplemented condition. Typical lentoid bodies were developed after about 2 weeks of serum-free culture. Thus, we can clearly demonstrate that the chicken embryonic pigmented epithelial cells do not always require a full set of serum factors for their transdifferentiation to lens cells in vitro.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Kosaka J,Watanabe K,Eguchi Gdoi
10.1016/0014-4835(92)90190-4keywords:
subject
Has Abstractpub_date
1992-08-01 00:00:00pages
261-7issue
2eissn
0014-4835issn
1096-0007pii
0014-4835(92)90190-4journal_volume
55pub_type
杂志文章abstract::Contact inhibition is an important mechanism for maintaining corneal endothelium in a non-replicative state. Protein tyrosine phosphatases (PTPs) play a role in regulating the integrity of cell-cell contacts, differentiation, and growth. In this study, we aimed to evaluate whether phosphatases are involved in the main...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.03.015
更新日期:2005-11-01 00:00:00
abstract::The regulation of acidic amino-acid transport across the retinal pigment epithelium is of particular interest since glutamate and possibly aspartate have been identified as putative neurotransmitters in the retina, at the level of the photoreceptor cell. The present study, designed to measure the rate of acidic amino-...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(86)80088-4
更新日期:1986-08-01 00:00:00
abstract::This review summarizes the Ernst H. Bárány Prize Lecture given at the meeting of the International Society of Eye Research in Geneva 2002. In the paper the path from the author's early studies on neurogenic inflammation in the eye to the search for a suitable prostaglandin analogue for glaucoma treatment, and the deve...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2003.11.016
更新日期:2004-04-01 00:00:00
abstract::Binocular vision provides the most accurate and precise depth information; however, many people have impairments in binocular visual function. It is possible that other sensory inputs could be used to obtain reliable depth information when binocular vision is not available. However, it is currently unknown whether dep...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.08.013
更新日期:2019-06-01 00:00:00
abstract::The objective of this study was to determine the cellular localization of P2Y(2) receptor gene expression in rabbit and primate ocular tissues using the technique of non-isotopic in situ hybridization. Fresh frozen whole eye from a New Zealand White rabbit and whole eye and eyelid from a rhesus macaque were cut into 5...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(03)00068-x
更新日期:2003-07-01 00:00:00
abstract::VEGF secretion by the human retinal pigment epithelium (hRPE) plays an important role in retinal and choroidal neovascularization. In this study, transforming growth factor-beta2 (TGF-beta2)-induced vascular endothelial growth factor (VEGF) gene expression was investigated in hRPE cells. Treatment of hRPE cells with T...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.12.016
更新日期:2007-05-01 00:00:00
abstract::Form-deprivation of chicks during early postnatal development results in ocular enlargement and great myopic refractive error (form-deprivation myopia). Previous studies have indicated that the retina, RPE and choroid play important roles in ocular enlargement in form-deprivation myopia. We aimed to isolate genes up-r...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1999.0765
更新日期:2000-02-01 00:00:00
abstract::We designed an experiment to study the role of light history and polyunsaturated fatty acids (PUFA) on the susceptibility of the albino rat retina to light damage. Albino rats were born to dams that had been kept in either 1 lx or 250 lx cyclic light for 0-4 days prior to delivery and fed one of three diets containing...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(05)80112-5
更新日期:1995-03-01 00:00:00
abstract::Lens lipid composition and lipid hydrocarbon chain structure change with age, region and cataract. Since the lens Ca(2+)-ATPase pump is important to the maintenance of calcium homeostasis and lens clarity, muscle sarcoplasmic reticulum Ca(2+)-ATPase was reconstituted with bovine lens lipids and dihydrosphingomyelin, t...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1999.0703
更新日期:1999-09-01 00:00:00
abstract::The current study investigates the cellular events which trigger activation of proapoptotic Bcl-2-associated × protein (Bax) in retinal cell death induced by all-trans-retinal (atRAL). Cellular events which activate Bax, such as DNA damage by oxidative stress and phosphorylation of p53, were evaluated by immunochemica...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2014.04.003
更新日期:2014-06-01 00:00:00
abstract::Primary cultures of chick lens annular pad (CLAP) cells were exposed to beta-adrenergic drugs and lipid soluble cAMP analogues. In dose-dependent manners, these agents inhibited re-entry into the cell cycle when these normally post-mitotic cells were cultured in the presence of serum. The inhibition of cell division w...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1131
更新日期:1993-09-01 00:00:00
abstract::Forskolin, a diterpene, a potent and direct stimulator of the catalytic unit of adenylate cyclase, can reduce intraocular pressure when administered topically to rabbit eyes in a 1% suspension. Using an intravitreal plant of fluorescein-dextran, aqueous flow was measured by a modification of the method of Maurice. For...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90113-1
更新日期:1984-07-01 00:00:00
abstract::Matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF) are all implicated in the development of neovascularization. To investigate the possible role of these factors in corneal neovascularization we have analysed the expression of MMP-2, MMP-9 and VEGF in a...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1999.0790
更新日期:2000-04-01 00:00:00
abstract::Retinal pigment epithelial cells (RPEs) are thought to be one of the main components of fibrous membrane observed in eyes with proliferative vitreo-retinopathy. We investigated the signalling mechanisms of TGF-beta2-dependent collagen gel contraction by RPEs. An in vitro type I collagen gel contraction assay was perfo...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.09.024
更新日期:2006-05-01 00:00:00
abstract::Endocannabinoid system involves in neuroprotective effects on the central neural system. The cannabinoid receptor 1 (CB1R) is widely expressed in the mouse retina. However, the role of cannabinoid receptors in the retina remains unclear. In this work, we established a photoreceptor degeneration mouse model via N-methy...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2017.11.001
更新日期:2018-02-01 00:00:00
abstract::We have previously shown that corticotropin-releasing hormone plays an important proinflammatory role in the induction of experimental autoimmune uveoretinitis. In this study, we examined the role of apoptosis in the destruction of the retina during experimental autoimmune uveoretinitis, and the role of corticotropin-...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2001.0992
更新日期:2001-06-01 00:00:00
abstract::In order to determine whether neuronal cell death in the retina accompanies senescence, retinas from pigmented ACI rats of various ages were analysed for changes in dimensions, cell densities, DNA content, and rhodopsin levels. Between the ages of 4 and 30 months, there were significant declines in the densities of nu...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(86)90022-9
更新日期:1986-04-01 00:00:00
abstract::Myopia (short-sightedness) is a visual problem associated with excessive eye growth and vitreous chamber expansion. Within the eye serotonin (5-hydroxytryptamine, 5-HT) appears to have a variety of effects, it alters retinal amacrine cell processing, increases intraocular pressure, constricts ocular blood vessels, and...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.03.021
更新日期:2005-11-01 00:00:00
abstract::Glaucoma challenges the survival of retinal ganglion cell axons in the optic nerve through processes dependent on both aging and ocular pressure. Relevant stressors likely include complex interplay between axons and astrocytes, both in the retina and optic nerve. In the DBA/2J mouse model of pigmentary glaucoma, early...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.11.016
更新日期:2016-09-01 00:00:00
abstract::Cataract is a multifactorial disease, and a large variety of stressors induce cataracts. Many cataractogenic stressors and endoplasmic reticulum (ER) stressors induce the unfolded protein response (UPR) in various cell types. The UPR is known to produce reactive oxygen species (ROS) prior to the inducement of apoptosi...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.01.033
更新日期:2006-09-01 00:00:00
abstract::Dysfunctional vascular endothelial nitric oxide synthase (eNOS) has been proposed to play a main pathophysiological role in various ocular diseases. The aim of the present study was to test the hypothesis that the chronic lack of eNOS impairs endothelium-dependent vasodilation in retinal arterioles. The relevance of e...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.01.022
更新日期:2019-04-01 00:00:00
abstract::Injection of herpes simplex virus vectors into the vitreous of primate eyes induces an acute, transient uveitis. The purpose of this study was to characterize innate immune responses of macaque neural retina tissue to the herpes simplex virus type 1-based gene delivery vector hrR3. PCR array analysis demonstrated the ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2016.05.003
更新日期:2016-07-01 00:00:00
abstract::This study was performed to further characterize expression and function of the Fas/Fas ligand system in the cornea. Specifically, these experiments examined (1) the effect of genetic inactivation of Fas or Fas ligand genes on keratocyte apoptosis in response to corneal epithelial wounding, (2) whether cultured human ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0371
更新日期:1997-10-01 00:00:00
abstract::Ischemic preconditioning (IPC) protects the rat retina against the injury that ordinarily follows severe ischemia. We showed previously that release of adenosine and de novo protein synthesis were required for IPC protection. The mechanisms of IPC were studied in the rat retina by examining the signal transduction med...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2000.0843
更新日期:2000-06-01 00:00:00
abstract::Proliferative diabetic retinopathy (PDR) is characterized by intraocular formation of fibroglial vascularized tissue by active vasoproliferative mechanisms. Using immunocytochemistry, we have studied changes in the distribution pattern of fibronectin (FN), laminin (LN), vitronectin (VN) and their receptors in the newl...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(05)80079-x
更新日期:1995-01-01 00:00:00
abstract::Na,K-ATPase was detected in crude microsomes of guinea-pig retinal layers, cornea and lens by immunoblotting using antibodies generated against purified kidney Na,K-ATPase. The antiserum cross-reacted with the alpha catalytic subunit of the enzyme from the eye tissues but not the beta glycoprotein subunit, suggesting ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(85)90136-8
更新日期:1985-05-01 00:00:00
abstract:PURPOSE:To investigate the potential protective effect of novel G protein coupled estrogen receptor (GPER1) against the neurotoxicity induced by NMDA in the mouse retina. METHODS:We induce retinal ganglion cells (RGCs) toxic injury through intravitreal injection of NMDA or acute ocular hypertension (AOH) induced by an...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.107826
更新日期:2019-12-01 00:00:00
abstract::The aqueous humor is produced by the epithelium of the ciliary body, a complex structure encircling the anterior segment of the eye. Aqueous humor production occurs by active transport, but the mechanism of this process is not understood. To produce a preparation in which active transport can be investigated, we have ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1156
更新日期:1993-11-01 00:00:00
abstract::Homocysteine is an amino acid required for the metabolism of methionine. Excess homocysteine is implicated in cardiovascular and neurological disease and new data suggest a role in various retinopathies. Mice lacking cystathionine-beta-synthase (cbs(-/-)) have an excess of retinal homocysteine and develop anatomical a...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2011.12.011
更新日期:2012-03-01 00:00:00
abstract::Ultraviolet A (UVA) light-based photoactivation of riboflavin (Rf) to induce corneal crosslinking (CXL) and mechanical stiffening is now a well-known treatment for corneal ectasia and Keratoconus that is being used in a topographically guided photorefractive intrastromal CXL (PiXL) procedure to treat low degrees of re...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2020.108199
更新日期:2020-10-01 00:00:00