Abstract:
:Multidrug resistance associated protein (MRP) is a major family of efflux transporters involved in drug efflux leading to drug resistance. The objective of this study was to explore physical barriers for ocular drug absorption and to verify if the role of efflux transporters. MRP-2 is a major homologue of MRP family and found to express on the apical side of cell membrane. Cultured Rabbit Corneal Epithelial Cells (rCEC) were selected as an in vitro model for corneal epithelium. [14C]-erythromycin which is a proven substrate for MRP-2 was selected as a model drug for functional expression studies. MK-571, a known specific and potent inhibitor for MRP-2 was added to inhibit MRP mediated efflux. Membrane fraction of rCEC was used for western blot analysis. Polarized transport of [14C]-erythromycin was observed in rCEC and transport from B-->A was significantly high than from A-->B. Permeability's increased significantly from A-->B in the presence of MK-571 and ketoconozole. Uptake of [14C]-erythromycin in the presence of MK-571 was significantly higher than control in rCEC. RT-PCR analysis indicated a unique and distinct band at approximately 498 bp corresponding to MRP-2 in rCEC and MDCK11-MRP-2 cells. Immunoprecipitation followed by Western Blot analysis indicated a specific band at approximately 190 kDa in membrane fraction of rCEC and MDCK11-MRP-2 cells. For the first time we have demonstrated high expression of MRP-2 in rabbit corneal epithelium and its functional activity causing drug efflux. RT-PCR, immunoprecipitation followed by Western blot analysis further confirms the result.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Karla PK,Pal D,Mitra AKdoi
10.1016/j.exer.2006.08.015subject
Has Abstractpub_date
2007-01-01 00:00:00pages
53-60issue
1eissn
0014-4835issn
1096-0007pii
S0014-4835(06)00367-8journal_volume
84pub_type
杂志文章abstract::Electron-microscope immunocytochemistry and antibody staining of nitrocellulose replicas of SDS gels (Western blots) were used in a developmental study to detect the presence and localization of opsin in the developing photoreceptors of rds (020/A) mutant mice and their BALB/c normal controls. Western blot analysis of...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80061-1
更新日期:1987-10-01 00:00:00
abstract::As a potential drug for the prevention of secondary cataract formation (SCF), we investigated the effect of Aclacinomycin A (ACM) on the growth of cultures of porcine lens epithelial cells in vitro. ACM is an anthracycline that has been used in the treatment of acute myeloid leukemia in the human for many years. It ha...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::The anterior avian cornea possesses several distinct cellular and extracellular regions including the epithelial basal lamina, Bowman's layer and the interfacial matrix that separates Bowman's layer from the stroma. These unique regions differ biochemically, physically and morphologically but all contain type XII coll...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2002.2058
更新日期:2002-12-01 00:00:00
abstract::Mammalian lens development involves cell-cell and cell-ECM interactions. As integrins are a major family of cell adhesion molecules, we examined the expression patterns of several integrin subunits (alpha3A, alpha3B, alpha6A, alpha6B, beta1 and beta4) during rat lens development. RT-PCR, in situ hybridisation, immunof...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.02.005
更新日期:2005-09-01 00:00:00
abstract::Retinal stimulation with high spatial resolution requires close proximity of electrodes to target cells. This study examines the effects of material coatings and 3-dimensional geometries of subretinal prostheses on their integration with the retina. A trans-scleral implantation technique was developed to place microfa...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2008.09.018
更新日期:2009-01-01 00:00:00
abstract::Exposure of the rabbit lens, in vitro, to 10(-3) M-hydrogen peroxide resulted in an increase of the lens sodium, calcium and water content together with a decrease of the lens potassium content. No such changes were observed in the presence of 10(-4) or 10(-5) M-hydrogen peroxide. 10(-3) M-hydrogen peroxide was observ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(83)90148-3
更新日期:1983-07-01 00:00:00
abstract::This study was conducted to compare the effects of two anti-VEGF-A drugs, Ranibizumab and Aflibercept, on the expression and secretion of VEGFs family members, and on their influence in proliferation and migration of endothelial cells (HECV) in vitro. HECV cells were exposed 24 h (T1), 4 days (T2) and 6 days (T3) to R...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.12.014
更新日期:2016-05-01 00:00:00
abstract::Limbal transplants in humans show a high rate of rejection even under local and systemic immunotherapy. In order to test immunomodulatory treatments a new limbal transplant model in the rat was developed using enhanced green fluorescent protein (E-GFP) as marker for follow-up. Sixty E-GFP-positive limbal transplants f...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.06.006
更新日期:2006-11-01 00:00:00
abstract::The retinal pigment epithelial cell has several important functions, one of which is the phagocytosis of photoreceptor outer segments which are discarded diurnally. We previously provided evidence in human retinal pigment epithelium that CD36, an 88 kDa integral membrane glycoprotein, participates in the phagocytosis ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0177
更新日期:1997-01-01 00:00:00
abstract::A proteinase from human lens membrane was purified by a procedure previously developed for a similar proteinase from bovine lenses (Srivastava, 1988a). The purification of the human proteinase was achieved by solubilization of the enzyme from membranes with 2% sodium deoxycholate followed by two consecutive passages t...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(89)80067-3
更新日期:1989-02-01 00:00:00
abstract::By methods of subtraction-hybridization of lambda ZAP cDNA libraries, prepared from 4-week-old rat lens epithelial cells (capsule) and lens fiber cells (decapsulated lens), we have isolated a specific cDNA clone whose target mRNA is about 600 b long. Northern blot hybridization analysis data showed that the target mRN...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(05)80009-0
更新日期:1995-06-01 00:00:00
abstract::Over the past 40 years, several groups have shown that epithelial debridement results in the death of keratocytes subjacent to the wound area. More recently this cell death has been shown to involve apoptosis. The purpose of this project was to examine the proliferative response of the normally quiescent keratocytes t...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2000.0926
更新日期:2001-01-01 00:00:00
abstract::Ischemic preconditioning (IPC) protects the rat retina against the injury that ordinarily follows severe ischemia. We showed previously that release of adenosine and de novo protein synthesis were required for IPC protection. The mechanisms of IPC were studied in the rat retina by examining the signal transduction med...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2000.0843
更新日期:2000-06-01 00:00:00
abstract::This study investigated the changes in pH during retinal artery occlusion by means of extracellular H+ concentration ([H+]o) measurements in the retina under both air and 100% O2 ventilation. Occlusion was produced in intact anesthetised cats by pressing with a probe onto a retinal artery. [H+]o profiles were recorded...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.11.002
更新日期:2005-04-01 00:00:00
abstract::Gene therapy has been proposed as a feasible strategy for RGC survival and optic nerve regeneration. Some preclinical and clinical studies revealed intraocular inflammation after intravitreal injection of adeno-associated virus (AAV) by slit-lamp or indirect ophthalmoscope. Here we evaluate the longitudinal profile of...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.107955
更新日期:2020-04-01 00:00:00
abstract::The effects of metabolic inhibitors on the apical and basal membrane potentials were studied in the isolated retinal pigment epithelium of the lizard Gekko gekko. Adding dinitrophenol or cyanide or cooling the tissue to 15 degrees C first depolarized the apical membrane and then hyperpolarized the basal membrane. The ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90015-m
更新日期:1990-01-01 00:00:00
abstract::Between 5 and 70% of genetic disease is caused by in-frame nonsense mutations, which introduce a premature termination codon (PTC) within the disease-causing gene. Consequently, during translation, non-functional or gain-of-function truncated proteins of pathological significance, are formed. Approximately 50% of all ...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2017.01.001
更新日期:2017-02-01 00:00:00
abstract::Homocysteine is an amino acid required for the metabolism of methionine. Excess homocysteine is implicated in cardiovascular and neurological disease and new data suggest a role in various retinopathies. Mice lacking cystathionine-beta-synthase (cbs(-/-)) have an excess of retinal homocysteine and develop anatomical a...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2011.12.011
更新日期:2012-03-01 00:00:00
abstract::The effect of dietary supplementation with acetylsalicylic acid on the increased modification, and susceptibility to modification, of lens crystallins from the streptozocin diabetic rat, has been determined. This was done by the measurement of characteristic markers of protein post-translational oxidative modification...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1186
更新日期:1993-12-01 00:00:00
abstract::An attachment to (Haag-Streit and Topcon) slit-lamps is described. This permits projection of a patterned target into the eye and the visual or photographic analysis of the details of the image reflected from the posterior surface of the crystalline lens. A numerical assessment can then be made of the optical quality ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(92)90124-b
更新日期:1992-09-01 00:00:00
abstract::Optic fissure closure defects result in uveal coloboma, a potentially blinding condition affecting between 0.5 and 2.6 per 10,000 births that may cause up to 10% of childhood blindness. Uveal coloboma is on a phenotypic continuum with microphthalmia (small eye) and anophthalmia (primordial/no ocular tissue), the so-ca...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2020.107940
更新日期:2020-04-01 00:00:00
abstract::We studied the early protein profile in the ocular tissue extracted after LASIK and SMILE surgery. SMILE and LASIK was performed in contralateral eyes and stromal tissue samples were collected from 10 eyes of 5 donors. The stromal tissue samples were analyzed using label free quantification approach and ITRAQ labellin...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.107936
更新日期:2020-03-01 00:00:00
abstract::The ability of interphotoreceptor retinoid-binding protein (IRBP) to facilitate the incorporation of retinol into retinyl esters by the retinal pigment epithelium (RPE) was examined in toad (Bufo marinus) eyecup preparations devoid of neural retina (RPE-eyecup). Solutions containing purified bovine IRBP and all-trans[...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(89)80059-4
更新日期:1989-10-01 00:00:00
abstract::Photoreceptor outer segments (OS) in the vertebrate retina undergo a process of continual renewal involving shedding of disc membranes that are cleared by phagocytic uptake into the retinal pigment epithelium (RPE). In dystrophic Royal College of Surgeons (RCS) rats, OS phagocytosis is blocked by a mutation in the gen...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.08.006
更新日期:2015-11-01 00:00:00
abstract::Immunofluorescent imaging is an indispensable technique to study morphology and molecular aspects in tissues. Classical approaches make it necessary to cut physical sections of tissue samples to overcome the limited penetration depth of light, restricting the available information to two dimensions. Recent advances in...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.12.001
更新日期:2019-03-01 00:00:00
abstract::The objective of the study was to investigate the effects of baicalin, baicalein, and wogonin (plant flavonoids) on interleukin-6 (IL-6) and interleukin-8 (IL-8) protein production, mRNA expression, and nuclear factor-kappaB (NF-kappaB) binding activities induced by interleukin-1beta (IL-1beta) in human retinal pigmen...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(03)00116-7
更新日期:2003-08-01 00:00:00
abstract::This study evaluated the efficacy of Plasma rich in growth factors (PRGF-Endoret) on the corneal wound healing process after Photorefractive keratectomy (PRK). To address this, blood from three healthy donors was collected, centrifuged and, the whole plasma column (WP) and the plasma fraction with the highest platelet...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.07.007
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND:Absence of collagen VII causes blistering of the skin, eyes and many other tissues. This disease is termed dystrophic epidermolysis bullosa (DEB). Corneal fibrosis occurs in up to 41% and vision loss in up to 64% of patients. Standard treatments are supportive and there is no cure. The hypomorphic mouse mode...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.06.008
更新日期:2018-10-01 00:00:00
abstract::The purpose of this study was to examine the localization and relative levels of vascular endothelial growth factor (VEGF; an angiogenic factor) and pigment epithelium-derived factor (PEDF; an antiangiogenic factor) in aged human choroid and to determine if the localization or their relative levels changed in age-rela...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.05.007
更新日期:2006-01-01 00:00:00
abstract::Injection of herpes simplex virus vectors into the vitreous of primate eyes induces an acute, transient uveitis. The purpose of this study was to characterize innate immune responses of macaque neural retina tissue to the herpes simplex virus type 1-based gene delivery vector hrR3. PCR array analysis demonstrated the ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2016.05.003
更新日期:2016-07-01 00:00:00