Abstract:
:As a potential drug for the prevention of secondary cataract formation (SCF), we investigated the effect of Aclacinomycin A (ACM) on the growth of cultures of porcine lens epithelial cells in vitro. ACM is an anthracycline that has been used in the treatment of acute myeloid leukemia in the human for many years. It has been shown to be non-mutagenic and non-carcinogenic in in vitro and in animal models. Subconfluent cell cultures were exposed to different concentrations of ACM for 5 minutes. The drug effect was evaluated by cell counts after various lengths of culture time (between 1 and 10 weeks). No cells survived the treatment with 12 or 16 microg ml-1. Cultures treated with concentrations between 0.5 and 8 microg ml-1 showed a marked decrease in cell number when compared to controls. However, reproliferation occurred at concentration up to 8 microg ml-1 after 2-6 weeks. Intraocular application of ACM might be suitable in the prevention of SCF. However, with regard to reproliferation, long-term cultures (or long-term animal models, respectively) have to be used in further evaluating the appropriate dosage for this purpose.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Schmidt JF,Meyer JH,Fluegel B,Hansen LL,Loeffler KUsubject
Has Abstractpub_date
1998-01-01 00:00:00pages
43-7issue
1eissn
0014-4835issn
1096-0007journal_volume
66pub_type
杂志文章abstract::The P23H-1 rat strain carries a rhodopsin mutation frequently found in retinitis pigmentosa patients. We investigated the progressive degeneration of the inner retina in this strain, focussing on retinal ganglion cells (RGCs) fate. Our data show that photoreceptor death commences in the ventral retina, spreading to th...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2010.10.003
更新日期:2010-12-01 00:00:00
abstract::Tissue kallikrein is a serine proteinase which processes kininogens to release bioactive kinins. Kinins mediate a variety of biological processes through the interaction with kinin receptors. Kinins are involved in the regulation of blood pressure and local blood flow, vasodilation, smooth muscle contraction and relax...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0087
更新日期:1996-07-01 00:00:00
abstract::This study tests the hypothesis that choroidal myogenic autoregulation participates in the intraocular pressure (IOP) response to mean arterial pressure (MAP) by minimizing arterial-pressure-dependent changes in choroidal blood volume. To test this hypothesis, the IOP response to MAP was quantified before and after im...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1047
更新日期:1994-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(86)90025-4
更新日期:1986-04-01 00:00:00
abstract::The purpose of this study was to determine the aqueous levels and pharmacokinetics of topical fluconazole 0.2% upon single and multiple drop applications. Forty-nine patients undergoing cataract surgery were given topical fluconazole 0.2%. They either received single drop or a loading dose of 1 drop per 5 min for 20 m...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2000.0936
更新日期:2001-02-01 00:00:00
abstract::Post-translational modifications by transglutaminase may contribute to the remodeling of cellular architecture in the development of lens fiber cells, and there is evidence that the enzyme may also play a role in cataract formation. It catalyses hydrolytic deamidations as well as amide exchanges on select glutamine si...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0463
更新日期:1998-05-01 00:00:00
abstract::A number of different agents, such as growth factors, cytokines and phorbol esters have been shown to modulate trabecular meshwork cell function. These studies were designed to evaluate the role extracellular signal-regulated kinase (ERK) pathway plays in mediating the responses to platelet-derived growth factor-BB (P...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2001.1007
更新日期:2001-07-01 00:00:00
abstract::Colonization by Staphylococcus aureus (S. aureus) has been implicated in many infectious and wound healing disorders. This study was performed to characterize the pathogenic role of S. aureus alpha-hemolysin (alpha-toxin) in corneal epithelial wound healing and infectious keratitis in the setting of a corneal wound. T...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.02.019
更新日期:2019-04-01 00:00:00
abstract::Previous evidence has shown clearly that sugar cataract formation results from unusually high intracellular levels of polyol. Documentation of polyol-related histological changes in the cortical fiber cells of the equatorial zone has been extensive. However, little attention has been given to the early changes in the ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90107-6
更新日期:1990-06-01 00:00:00
abstract::Obesity has been associated with abnormal lipid metabolism and with tissue hypoxia. Human Bruch's membrane (BrM) lipid deposits have been proposed to create a diffusion barrier to metabolic exchange between the choroid and photoreceptors, delaying the regeneration of photopigments. The speed of retinal dark adaptation...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.02.007
更新日期:2019-04-01 00:00:00
abstract::Movement of water in the lens, specifically water diffusion, was examined with diffusion imaging and H217O-enhanced proton imaging. Diffusion imaging showed restricted water self-diffusion in the cortex which was not concurrent with the change in proton density; nevertheless it was dependent on the gradient direction ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(92)90076-5
更新日期:1992-01-01 00:00:00
abstract::Periorbital adipose tissue expansion is a key pathological change in thyroid associated orbitopathy (TAO). Bone morphogenic protein 4 (BMP4) is instrumental in adipogenesis. We compared site-specific BMP4 expression and its effect on adipogenesis using donor-matched adipose tissue-derived stromal cells (ADSC) from TAO...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.01.020
更新日期:2019-04-01 00:00:00
abstract::A substantial change in the glycoconjugates of the rat interphotoreceptor matrix (IPM) has recently been shown to occur between post-natal day (P) 14 and P16 in rat retinas using lectin histochemistry. This suggests that the sialic acid content on the terminus of N-glycoside linked carbohydrate chains of the IPM incre...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1013
更新日期:1993-01-01 00:00:00
abstract::Much controversy has arisen on the nature and sources of stem cells in the adult human retina. Whilst ciliary epithelium has been thought to constitute a source of neural stem cells, a population of Müller glia in the neural retina has also been shown to exhibit neurogenic characteristics. This study aimed to compare ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2011.09.015
更新日期:2011-12-01 00:00:00
abstract::We aim to determine whether lymphatic drainage from the eye changes with age. Using quantitative photoacoustic tomography, groups of young and older mice were studied in the live state. 10 CD-1 mice of 2-3 months (5M/5F) were studied in addition to 13 older mice of 12-13 months (6M/7F). In each of 23 mice, near-infrar...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.108029
更新日期:2020-05-01 00:00:00
abstract::The aim of this study was to identify the molecular genetic cause of disease in posterior polymorphous corneal dystrophy (PPCD) probands of diverse origin and to assess the utility of massively parallel sequencing in the detection of ZEB1 mutations. We investigated a total of 12 families (five British, four Czech, one...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.03.002
更新日期:2019-05-01 00:00:00
abstract::We have examined the mercapturic acid pathway of the cataractous rabbit lens following induction by naphthalene as an oxidative foreign substance. 1,2-Naphthoquinone, which is formed in the eye from naphthalene diol and other naphthalene derivatives by a combination of enzymic and non-enzymic reactions, readily oxidiz...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(88)90058-9
更新日期:1988-09-01 00:00:00
abstract::In a variety of tissues including gastrointestinal mucosa, rebamipide (REB) provides cytoprotection, prevents inflammation, and promotes wound healing. Clinically, REB ophthalmic dispersions are used to treat diabetic keratopathy. In this study, we investigated the optimal particle size of REB to promote corneal wound...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2016.07.003
更新日期:2016-10-01 00:00:00
abstract::The association between the use of statins and age-related macular degeneration (AMD), a leading cause of blindness, has been evaluated in many clinical studies; however, the results have been contradictory. We evaluated the effect of pitavastatin administration on laser-induced experimental choroidal neovascularizati...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.02.005
更新日期:2007-06-01 00:00:00
abstract::Dysfunctional vascular endothelial nitric oxide synthase (eNOS) has been proposed to play a main pathophysiological role in various ocular diseases. The aim of the present study was to test the hypothesis that the chronic lack of eNOS impairs endothelium-dependent vasodilation in retinal arterioles. The relevance of e...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.01.022
更新日期:2019-04-01 00:00:00
abstract::Argon laser energy was applied to the trabecular meshwork of pigmented rabbits in an attempt to develop an animal model of 'glaucoma'. Laser energy was varied to determine the optimal level needed to produce sustained ocular hypertension. An initial response of ocular hypertension followed by hypotension was observed ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(86)90067-9
更新日期:1986-12-01 00:00:00
abstract:PURPOSE:To characterize multilamellar bodies (MLBs), determine their distribution along the optic axis and predict their potential Mie scattering within human age-related nuclear cataracts. Previous studies restricted to the equatorial plane have shown that MLBs are rare spherical objects that are 1-4 microm in diamete...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.05.017
更新日期:2004-10-01 00:00:00
abstract::We showed previously that treatment of cultured rabbit lens epithelial cells (LECs) with hyperbaric oxygen (HBO) produced DNA strand-breaks, caused reversible inhibition of protein synthesis and induced the synthesis of a 32 kD protein. In the present work, we employed immunostaining procedures to identify the 32 kD p...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0356
更新日期:1997-09-01 00:00:00
abstract::Ultraviolet A (UVA) light-based photoactivation of riboflavin (Rf) to induce corneal crosslinking (CXL) and mechanical stiffening is now a well-known treatment for corneal ectasia and Keratoconus that is being used in a topographically guided photorefractive intrastromal CXL (PiXL) procedure to treat low degrees of re...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2020.108199
更新日期:2020-10-01 00:00:00
abstract::Osteopontin (OPN) is elevated during the progression of experimental autoimmune uveoretinitis (EAU) in C57BL/6 (B6) mice. Furthermore, EAU symptoms are ameliorated in OPN knockout mice or in B6 mice treated with anti-OPN antibody (M5). Recently, OPN has been shown to promote the Th1 response not only in the extracellu...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2009.09.008
更新日期:2010-01-01 00:00:00
abstract::Bombina variegata 8 (Bv8), also known as prokineticin-2 (PK-2), is a potent pro-angiogenic factor. However, its role in retinal neovascularization (RNV) remains unknown. In this study, we explored the role of Bv8 in the pathogenesis of RNV. We found that the expression of Bv8 was significantly increased in two differe...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.107993
更新日期:2020-04-01 00:00:00
abstract::Familial exudative vitreoretinopathy (FEVR) is a disease exhibits a wide range of clinical signs, ranging mild peripheral retinal vascular anomalies to severe retinal detachments. Individuals with mild FEVR are frequently asymptomatic with good visual function and are often undiagnosed. However, little is known about ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.107941
更新日期:2020-03-01 00:00:00
abstract::Atropine, a non-selective muscarinic receptor antagonist, is currently the most potent agent used to prevent myopia in animal models and children. However, the ocular target tissues are not well defined. To learn more about the effect of atropine on experimental myopia, atropine was applied both intravitreally and sys...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.09.019
更新日期:2007-02-01 00:00:00
abstract::N-methyl-D-aspartate (NMDA)-induced excitotoxicity is an acute form of experimental retinal injury as a result of overactivation of glutamate receptors. NLRP3 (nucleotide-binding domain, leucine-rich-repeat containing family, pyrin domain containing-3) inflammasome, one of the most studied sensors of innate immunity, ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.01.018
更新日期:2019-04-01 00:00:00
abstract::The aim of this study was to investigate the developmental potential of embryonic stem (ES) cell-derived eye-like structures as a replacement cell therapy model for retinas with N-methyl-d-aspartate (NMDA)-induced damage. For this purpose mouse ES cells were induced to differentiate into eye-like structures in vitro f...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.01.007
更新日期:2007-05-01 00:00:00